Production of pectinases by solid-state fermentation of a mixture of citrus waste and sugarcane bagasse in a pilot-scale packed-bed bioreactor

2016 ◽  
Vol 111 ◽  
pp. 54-62 ◽  
Author(s):  
Alessandra Biz ◽  
Anelize Terezinha Jung Finkler ◽  
Luana Oliveira Pitol ◽  
Bruna Schweitzer Medina ◽  
Nadia Krieger ◽  
...  
2016 ◽  
Vol 283 ◽  
pp. 1009-1018 ◽  
Author(s):  
Luana Oliveira Pitol ◽  
Alessandra Biz ◽  
Edgar Mallmann ◽  
Nadia Krieger ◽  
David Alexander Mitchell

Author(s):  
David Alexander Mitchell ◽  
Luana Oliveira Pitol ◽  
Alessandra Biz ◽  
Anelize Terezinha Jung Finkler ◽  
Luiz Fernando de Lima Luz ◽  
...  

2017 ◽  
Vol 121 ◽  
pp. 1-12 ◽  
Author(s):  
Anelize Terezinha Jung Finkler ◽  
Alessandra Biz ◽  
Luana Oliveira Pitol ◽  
Bruna Schweitzer Medina ◽  
Henrique Luithardt ◽  
...  

2020 ◽  
pp. 2097-2116 ◽  
Author(s):  
Priscila Aparecida Casciatori Frassatto ◽  
Fernanda Perpétua Casciatori ◽  
João Cláudio Thoméo ◽  
Eleni Gomes ◽  
Maurício Boscolo ◽  
...  

Cellulases are essential for the hydrolysis of lignocellulosic materials in the production of second generation ethanol. Solid-state cultivation is a process that provides high concentrations of enzymes that can be used in this hydrolysis. The objectives of this work were to produce cellulases by cultivating the fungus Myceliophthora thermophila I-1D3b in a packed bed bioreactor with sugarcane bagasse (SCB) and wheat bran (WB) as substrate and to evaluate the efficiency of the enzymatic extract in the hydrolysis of SCB in natura (BIN) and pretreated with ozone, alkali and ultrasound (BOU). The conditions for enzyme production in the bioreactor were SCB:WB at a ratio of 2.3:1 (w/w), 75 % moisture content; 45 ºC; aeration rate 240 L h-1 and 96 h. The enzyme production was evaluated by endoglucanase, xylanase, filter paper (FPU) and ?-glycosidase activities. For the application of the enzymes, a central composed response surface design with 5 repetitions of the central point was used, taking enzyme volume and hydrolysis time as factors. Such cultivation yielded the following enzymatic activities: 723 U gss-1 of endoglucanases, 2024 U gss-1 of xylanase, 12.6 U gss-1 of FPU and 41 U gss-1 of ?-glucosidase. The results of the application tests indicated the best conditions as 7.0 ml of the enzyme extract (4.2 FPU) and 6 hours for BIN and BOU. The best cellulose-glucose conversions were obtained for BOU, reaching 32.1 % at 65 ºC. In conclusion, the enzyme production in the packed bed bioreactor was efficient and BOU pretreatment improved the hydrolysis of biomass, increasing the efficiency of conversion of cellulose to glucose.


2009 ◽  
Vol 37 (4) ◽  
pp. 391-400 ◽  
Author(s):  
Marcio A. Mazutti ◽  
Giovani Zabot ◽  
Gabriela Boni ◽  
Aline Skovronski ◽  
Débora de Oliveira ◽  
...  

2010 ◽  
Vol 85 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Marcio A. Mazutti ◽  
Giovani Zabot ◽  
Gabriela Boni ◽  
Aline Skovronski ◽  
Débora de Oliveira ◽  
...  

1995 ◽  
Vol 30 (1) ◽  
pp. 9-15 ◽  
Author(s):  
M.K. Gowthaman ◽  
K.S.M.S.Raghava Rao ◽  
N.P. Ghildyal ◽  
N.G. Karanth

2017 ◽  
Vol 40 (7) ◽  
pp. 1123-1132 ◽  
Author(s):  
Felisbela Oliveira ◽  
José Manuel Salgado ◽  
Luís Abrunhosa ◽  
Noelia Pérez-Rodríguez ◽  
José M. Domínguez ◽  
...  

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