Streamlining Cell-Free Protein Synthesis Biosensors for use in Human Fluids: in situ RNase Inhibitor Production During Extract Preparation

2021 ◽  
pp. 108158
Author(s):  
Mehran Soltani ◽  
Bradley C. Bundy
Keyword(s):  
Protein Synthesis ◽  
Rnase Inhibitor ◽  
Free Protein ◽  
Human Fluids ◽  
Cell Free Protein Synthesis

scholarly journals Cell-free protein synthesis and in situ immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion

RSC Advances ◽  
2020 ◽  
Vol 10 (66) ◽  
pp. 40588-40596
Author(s):  
Tony Köhler ◽  
Thomas Heida ◽  
Sandra Hoefgen ◽  
Niclas Weigel ◽  
Vito Valiante ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Genetic Information ◽  
In Situ Immobilization ◽  
Bottom Up ◽  
Free Protein ◽  
Malonyl Coa ◽  
Cell Free Protein Synthesis

We describe a bottom-up approach towards functional enzymes utilizing microgels as carriers for genetic information that enable cell-free protein synthesis, in situ immobilization, and utilization of functional deGFP-MatB.

In Situ Deprotection and Incorporation of Unnatural Amino Acids during Cell-Free Protein Synthesis

2013 ◽  
Vol 19 (21) ◽  
pp. 6824-6830 ◽  
Author(s):  
Isaac N. Arthur ◽  
James E. Hennessy ◽  
Dharshana Padmakshan ◽  
Dannon J. Stigers ◽  
Stéphanie Lesturgez ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Unnatural Amino Acids ◽  
Free Protein ◽  
Cell Free Protein Synthesis

Protein In Situ Arrays through Cell-Free Protein Synthesis

2007 ◽  
pp. 133-143
Author(s):  
Farid Khan ◽  
Elizabeth Palmer ◽  
Mingyue He ◽  
Michael Taussig ◽  
Mingwei Wang
Keyword(s):  
Protein Synthesis ◽  
Free Protein ◽  
Cell Free Protein Synthesis
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