rnase inhibitor
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Eric Vaughn snRNASeq Protocol v1

2021 ◽  
Author(s):  
Eric Vaughn ◽  
Catherine Dulac
Keyword(s):  
Free Water ◽  
Rnase Inhibitor ◽  
Lysis Buffer

This protocol is designed to extract single nuclei for sequencing with 10X Genomics technologies. Note: For each step, keep tissue/nuclei/reagents/centrifuge on ice/cold to reduce IEG expression and maintain nuclear integrity. Buffers to prepare: -100mL Lysis Buffer Store @ 4 C: 98.4mL Nuclease-free water 1mL 1M Tris-HCl (10mM) 200uL 5M NaCl (10mM) 300uL 1M MgCl2 (3mM) 100uL NonIdet P40 (0.1%) -PBS w/ 1% BSA and RNAse inhibitor (new batch for each run; enough for 3-4 separate samples): 10mL PBS 100 mg powdered BSA (mix well) 50uL Promega RNasin (40U/uL; final conc: 0.2U/uL)

scholarly journals Structural Tailoring of a Novel Fluorescent IRE-1 RNase Inhibitor to Precisely Control Its Activity

2019 ◽  
Vol 62 (11) ◽  
pp. 5404-5413 ◽  
Author(s):  
Andong Shao ◽  
Chang Won Kang ◽  
Chih-Hang Anthony Tang ◽  
Christopher F. Cain ◽  
Qin Xu ◽  
...  
Keyword(s):  
Rnase Inhibitor

scholarly journals Polyvinylsulfonic acid: A Low-cost RNase inhibitor for enhanced RNA preservation and cell-free protein translation

Bioengineered ◽  
2017 ◽  
Vol 9 (1) ◽  
pp. 90-97 ◽  
Author(s):  
Conner C. Earl ◽  
Mark T. Smith ◽  
Richard A. Lease ◽  
Bradley C. Bundy
Keyword(s):  
Low Cost ◽  
Protein Translation ◽  
Rnase Inhibitor ◽  
Free Protein ◽  
Polyvinylsulfonic Acid ◽  
Rna Preservation

scholarly journals The expression and localization of RNase and RNase inhibitor in blood cells and vascular endothelial cells in homeostasis of the vascular system

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0174237 ◽  
Author(s):  
Ayaka Ohashi ◽  
Aya Murata ◽  
Yuichiro Cho ◽  
Shizuko Ichinose ◽  
Yuriko Sakamaki ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Cells ◽  
Vascular System ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Rnase Inhibitor

scholarly journals Data on the inhibition of RNase inhibitor activity by a monoclonal antibody as assessed by microfluidics-based RNA electrophoresis

Data in Brief ◽  
2016 ◽  
Vol 9 ◽  
pp. 417-421 ◽  
Author(s):  
Xiao Wang ◽  
Belete Teferedegne ◽  
Kenneth Shatzkes ◽  
Wei Tu ◽  
Haruhiko Murata
Keyword(s):  
Monoclonal Antibody ◽  
Inhibitor Activity ◽  
Rnase Inhibitor

scholarly journals Endogenous RNase inhibitor contributes to stability of RNA in crude cell lysates: Applicability to RT-qPCR

2016 ◽  
Vol 513 ◽  
pp. 21-27 ◽  
Author(s):  
Xiao Wang ◽  
Belete Teferedegne ◽  
Kenneth Shatzkes ◽  
Wei Tu ◽  
Haruhiko Murata
Keyword(s):  
Rnase Inhibitor ◽  
Cell Lysates

scholarly journals A novel fully human antitumor ImmunoRNase resistant to the RNase inhibitor

2012 ◽  
Vol 26 (3) ◽  
pp. 243-248 ◽  
Author(s):  
G. Riccio ◽  
C. D'Avino ◽  
R. T. Raines ◽  
C. De Lorenzo
Keyword(s):  
Rnase Inhibitor

scholarly journals Degradation by Stratum Corneum Proteases Prevents Endogenous RNase Inhibitor from Blocking Antimicrobial Activities of RNase 5 and RNase 7

2009 ◽  
Vol 129 (9) ◽  
pp. 2193-2201 ◽  
Author(s):  
Arby Abtin ◽  
Leopold Eckhart ◽  
Michael Mildner ◽  
Minoo Ghannadan ◽  
Jürgen Harder ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Antimicrobial Activities ◽  
Rnase Inhibitor
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