In Situ Deprotection and Incorporation of Unnatural Amino Acids during Cell-Free Protein Synthesis

2013 ◽  
Vol 19 (21) ◽  
pp. 6824-6830 ◽  
Author(s):  
Isaac N. Arthur ◽  
James E. Hennessy ◽  
Dharshana Padmakshan ◽  
Dannon J. Stigers ◽  
Stéphanie Lesturgez ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Unnatural Amino Acids ◽  
Free Protein ◽  
Cell Free Protein Synthesis

Translational incorporation of multiple unnatural amino acids in a cell-free protein synthesis system

2014 ◽  
Vol 19 (3) ◽  
pp. 426-432 ◽  
Author(s):  
Su-Jin Oh ◽  
Kyung-Ho Lee ◽  
Ho-Cheol Kim ◽  
Christy Catherine ◽  
Hyungdon Yun ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Unnatural Amino Acids ◽  
Synthesis System ◽  
Free Protein ◽  
A Cell ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

scholarly journals High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites

2012 ◽  
Vol 418 (4) ◽  
pp. 652-656 ◽  
Author(s):  
Kiyoshi Ozawa ◽  
Karin V. Loscha ◽  
Kekini V. Kuppan ◽  
Choy Theng Loh ◽  
Nicholas E. Dixon ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Unnatural Amino Acids ◽  
High Yield ◽  
Site Specific ◽  
Free Protein ◽  
Cell Free Protein Synthesis

scholarly journals Cell-free protein synthesis and in situ immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion

RSC Advances ◽  
2020 ◽  
Vol 10 (66) ◽  
pp. 40588-40596
Author(s):  
Tony Köhler ◽  
Thomas Heida ◽  
Sandra Hoefgen ◽  
Niclas Weigel ◽  
Vito Valiante ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Genetic Information ◽  
In Situ Immobilization ◽  
Bottom Up ◽  
Free Protein ◽  
Malonyl Coa ◽  
Cell Free Protein Synthesis

We describe a bottom-up approach towards functional enzymes utilizing microgels as carriers for genetic information that enable cell-free protein synthesis, in situ immobilization, and utilization of functional deGFP-MatB.

scholarly journals Cotranslational incorporation of non-standard amino acids using cell-free protein synthesis

FEBS Letters ◽  
2015 ◽  
Vol 589 (15) ◽  
pp. 1703-1712 ◽  
Author(s):  
Robert B. Quast ◽  
Devid Mrusek ◽  
Christian Hoffmeister ◽  
Andrei Sonnabend ◽  
Stefan Kubick
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Free Protein ◽  
Cell Free Protein Synthesis
Sign in / Sign up

Export Citation Format

Share Document