Electrospun sulfated silk fibroin nanofibrous scaffolds for vascular tissue engineering

Small caliber vascular replacement (<4 mm) still remains a challenge for medical and research teams, as no available vascular substitutes (VS) are suitable for small diameter bypass. Vascular engineering proposes new models of small diameter VS but rare are those that meet the biocompatibility and mechanical criteria. In this study, we developed a new scaffold made by the combination of two natural biomacromolecules: collagen and silk fibroin. The scaffold was further cellularised with porcine smooth muscle cells. First, the behavior of cells in the collagen-fibroin constructs was verified in order to evaluate the biocompatibility of the scaffold with the cells. Then, gel mass loss and cellular attachment, morphology, spreading and viability were analysed. The results showed an excellent interaction and biocompatibility between collagen, silk fibroin fibers and cells. Thus, the collagen-fibroin construct appears to be a very attractive material for vascular tissue engineering.

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Vascular Tissue Engineering Using Polycaprolactone Nanofibrous Scaffolds Fabricated via Electrospinning

Science of Advanced Materials ◽

10.1166/sam.2015.2252 ◽

2015 ◽

Vol 7 (3) ◽

pp. 407-413 ◽

Cited By ~ 5

Author(s):

Y. A. Elnakady ◽

Mohammed F. Al Rez ◽

H. Fouad ◽

Sarah Abuelreich ◽

Ahmed M. Albarrag ◽

...

Keyword(s):

Tissue Engineering ◽

Vascular Tissue ◽

Vascular Tissue Engineering ◽

Nanofibrous Scaffolds

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Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

Journal of Bioactive and Compatible Polymers ◽

10.1177/0883911520973244 ◽

2020 ◽

pp. 088391152097324

Author(s):

Xin Liu ◽

Bo Chen ◽

Yan Li ◽

Yan Kong ◽

Ming Gao ◽

...

Keyword(s):

Tissue Engineering ◽

Structural Stability ◽

Silk Fibroin ◽

Vascular Tissue ◽

Autologous Transplantation ◽

Vascular Tissue Engineering ◽

The Novel ◽

Long Distance ◽

Engineering Applications

Long-distance (⩾10 mm) arterial vascular defect injury was a massive challenge affecting human health. Compared with autologous transplantation, tissue-engineered scaffolds such as biocompatible silk fibroin (SF) scaffolds have been developed because they exhibit equivalent functional repair effects without adverse reactions. However, its mechanical strength and structural stability needed to be further improved to match the longer repair cycle of blood vessels while maintaining the original biological safety. Hence, we designed and prepared SF and hydrophobic polycaprolactone (PCL) composite microfibers by an improving electrospinning method. It was found that when the weight ratio of PCL to SF was 1: 1, a microfiber scaffold with high strength (6.16 N) and minimum degradability can be obtained. More importantly, compared with natural silk fibroin, the novel composite microfiber scaffolds can slightly inhibit cell infiltration and inflammation through co-culture with HUVECs in vitro and rabbit back transplantation in vivo. Furthermore, the fabricated scaffolds also demonstrated excellent structural stability in vivo because of the well-organized PCL doping in the structure. All these results indicated that the novel PCL/SF composite microfiber scaffolds were promising candidates for vascular tissue engineering applications.

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Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering

International Journal of Nanomedicine ◽

10.2147/ijn.s24312 ◽

2011 ◽

pp. 2133 ◽

Cited By ~ 78

Author(s):

Hadi Hajiali ◽

Shahgasempour ◽

Mohammad Reza Naimi-Jamal ◽

Peirovi

Keyword(s):

Tissue Engineering ◽

Potential Application ◽

Vascular Tissue ◽

Vascular Tissue Engineering ◽

Nanofibrous Scaffolds

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Strategies to Improve Nanofibrous Scaffolds for Vascular Tissue Engineering

Nanomaterials ◽

10.3390/nano10050887 ◽

2020 ◽

Vol 10 (5) ◽

pp. 887 ◽

Cited By ~ 3

Author(s):

Tianyu Yao ◽

Matthew B. Baker ◽

Lorenzo Moroni

Keyword(s):

Tissue Engineering ◽

Self Assembly ◽

Vascular Tissue ◽

Vascular Tissue Engineering ◽

In Vivo Evaluation ◽

Thermally Induced ◽

Vascular Regeneration ◽

Nanofibrous Scaffolds ◽

Biomimetic Scaffolds

The biofabrication of biomimetic scaffolds for tissue engineering applications is a field in continuous expansion. Of particular interest, nanofibrous scaffolds can mimic the mechanical and structural properties (e.g., collagen fibers) of the natural extracellular matrix (ECM) and have shown high potential in tissue engineering and regenerative medicine. This review presents a general overview on nanofiber fabrication, with a specific focus on the design and application of electrospun nanofibrous scaffolds for vascular regeneration. The main nanofiber fabrication approaches, including self-assembly, thermally induced phase separation, and electrospinning are described. We also address nanofibrous scaffold design, including nanofiber structuring and surface functionalization, to improve scaffolds’ properties. Scaffolds for vascular regeneration with enhanced functional properties, given by providing cells with structural or bioactive cues, are discussed. Finally, current in vivo evaluation strategies of these nanofibrous scaffolds are introduced as the final step, before their potential application in clinical vascular tissue engineering can be further assessed.

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