Abstract:
In recent times, several approaches for targeted gene therapy (GT) had been studied. However,
the emergence of extracellular vesicles (EVs) as a shuttle carrying genetic information between
cells has gained a lot of interest in scientific communities. Owing to their higher capabilities in dealing
with short sequences of nucleic acid (mRNA, miRNA), proteins, recombinant proteins, exosomes, the
most popular form of EVs are viewed as reliable biological therapeutic conveyers. They have natural
access through every biological membrane and can be employed for site-specific and efficient drug
delivery without eliciting any immune responses hence, qualifying as an ideal delivery vehicle. Also,
there are many research studies conducted in the last few decades on using exosome-mediated gene
therapy into developing an effective therapy with the concept of a higher degree of precision in gene
isolation, purification and delivery mechanism loading, delivery and targeting protocols. This review
discusses several facets that contribute towards developing an efficient therapeutic regime for gene
therapy, highlighting limitations and drawbacks associated with current GT and suggested therapeutic
regimes.
Targeted galectin-7 inhibition with ultrasound microbubble targeted gene therapy as a sole therapy to prevent acute rejection following heart transplantation in a Rodent model
