Dispersion optimization to enhance PHB production in fed-batch cultures of Ralstonia eutropha

In this study,Halomonas boliviensiswas cultivated on bakery waste hydrolysate and seawater in batch and fed-batch cultures for polyhydroxybutyrate (PHB) production. Results demonstrated that bakery waste hydrolysate and seawater could be efficiently utilized byHalomonas boliviensiswhile PHB contents between 10 and 30% (w/w) were obtained. Furthermore, three methods for bakery waste hydrolysis were investigated for feedstock preparation. These include: (1) use of crude enzyme extracts fromAspergillus awamori, (2)Aspergillus awamorisolid mashes, and (3) commercial glucoamylase. In the first method, the resultant free amino nitrogen (FAN) concentration in hydrolysates was 150 and 250 mg L−1after 20 hours at enzyme-to-solid ratios of 6.9 and 13.1 U g−1, respectively. In both cases, the final glucose concentration was around 130–150 g L−1. In the second method, the resultant FAN and glucose concentrations were 250 mg L−1and 150 g L−1, respectively. In the third method, highest glucose and lowest FAN concentrations of 170–200 g L−1and 100 mg L−1, respectively, were obtained in hydrolysates after only 5 hours. The present work has generated promising information contributing to the sustainable production of bioplastic using bakery waste hydrolysate.

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Optimal adaptive control schemes for PHB production in fed-batch fermentation of Ralstonia eutropha

Journal of Process Control ◽

10.1016/j.jprocont.2013.07.006 ◽

2013 ◽

Vol 23 (8) ◽

pp. 1159-1168 ◽

Cited By ~ 13

Author(s):

Wei Wu ◽

Song-Yi Lai ◽

Ming-Feng Jang ◽

Yi-Shyong Chou

Keyword(s):

Adaptive Control ◽

Batch Fermentation ◽

Ralstonia Eutropha ◽

Fed Batch ◽

Control Schemes ◽

Phb Production ◽

Fed Batch Fermentation

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Fed-batch production and characterization of polyhydroxybutyrate by Bacillus megaterium LVN01 from residual glycerol

DYNA ◽

10.15446/dyna.v87n214.83523 ◽

2020 ◽

Vol 87 (214) ◽

pp. 111-120

Author(s):

Javier Ricardo Gómez Cardozo ◽

Rodrigo Velasco Bucheli ◽

Nathalia Marín Pareja ◽

Orlando Simón Ruíz Villadiego ◽

Guillermo Antonio Correa Londoño ◽

...

Keyword(s):

Bacillus Megaterium ◽

Operating Conditions ◽

Fed Batch ◽

Fermentation Time ◽

Batch Cultures ◽

Batch Systems ◽

Dry Cell Weight ◽

Phb Production ◽

Dry Cell

The operating conditions of polyhydroxybutyrate (PHB) production processes are among the factors that most influence yields. In this study, we evaluated PHB production synthesized by Bacillus megaterium LVN01. Batch and fed-batch cultures were used to produce PHB from residual glycerol. For this, dry cell weight (DCW) and PHB productivity were analyzed at a preliminary stage by central composite design using batch systems under different temperature, C/N ratio, and fermentation time conditions. The maximum PHB productivity occurred at 30.8 °C, 44.9 mol mol-1, and 39.9 h. The same conditions were tested for studies in fed-batch culture. Fed-batch experiments were comparable to each other, where the DCW was around 1.9 g L-1, with PHB productivities of 29.5 mg L-1 h-1 and 35.6 mg L-1 h-1 for bioreactors of 5 L and 14 L, respectively. The PHB was characterized by NMR, FTIR, DSC, TGA, and DTG analys

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