The Influencing Factors on the Production of Alcoholic Drinking from Jackfruit (Artocarpus heterophyllus L.)

The large amount of jackfruit (Artocarpus heterophyllus Lam) harvested and their short use time caused many difficulties for the farmers. Fortunately, the high sugar content in jackfruit meat is a hopeful substance for wine production. This study aimed to consider the effect of yeast strains and their concentration on fermented jackfruit solution. Jackfruit juice with 14 °Brix is ​​fermented using 0.005 to 0.015% (w/v) Saccharomyces cerevisiae RV002, Mauri Instant Dry Yeast yeast under anaerobic conditions for 1 to 4 days at 30 °C. Survey samples were checked once a day to analyze the indicators. The functional report of the sugar in the fermentation time, shows that the higher incidence of yeast cultures and the initial sugar concentration inhibited yeast growth. The results showed that fermentation from jackfruit meat with 25 °Brix using Saccharomyces cerevisiae RV002 yeast with concentration of 0.01% for 3 days is the best to create a good quality with ethanol content 4,9% and characteristic aroma of jackfruit.

KARAKTERISTIK TEPUNG SORGUM TERMODIFIKASI DENGAN PROSES FERMENTASI DENGAN MENGGUNAKAN YEAST INDIGENOUS SORGUM

ABSTRACTThe purpose of this study was to examine the physicochemical characteristics of fermented sorghum flour using indigenous sorghum yeast. The study used a completely randomized factorial design with various fermentation times (24, 48, 72 hours) as the first factor and the concentration of indigenous sorghum yeast starter (2.5%, 5%, 7.5%, 10%, 12.5%, and 15% w/v) as the second factor in three replications. Parameters observed were physicochemical characteristics including starch content, water content, swelling power, water absorption, and bulk density. The data obtained were analyzed using analysis of variance (ANOVA) (P<0.05) and Duncan's Multiple Range Test (DMRT). The results show that the best-fermented sorghum flour was obtained at 72 hours of fermentation time with a 5% starter. The sorghum flour has a starch content of 74.8%, water content of 10.97%, swelling power of 15.44 g/g, water absorption capacity of 67.58%, and bulk density 0.6 g/ml. Modification by fermentation using indigenous sorghum yeast can improve the physical and chemical properties of sorghum flour. Based on the nutritional content of sorghum flour, it met the quality requirements of Codex Standard 173-1989 in ash, protein, fat, crude fiber, and carbohydrate contents.Keywords:sorghum, modified, yeast indigenous, phisycal and chemical characteristik.ABSTRAKTujuan penelitian ini adalah untuk mengkaji karakteristik fisikokimia tepung sorgum hasil fermentasi dengan menggunakan yeast indigenous sorgum. Penelitian dilakukan dengan menggunakan rancangan acak lengkap pola faktorial dengan lama fermentasi (24, 48, 72 jam) sebagai faktor pertama dan konsentrasi starter yeast indigenous sorgum (2,5%, 5%, 7,5% dan 10%, 12,5% dan 15% b/v) sebagai faktor kedua, menggunakan 3 ulangan. Parameter yang diamati adalah karakteristik fisikokimia meliputi kadar pati, kadar air, sweeling power, daya serap air dan densitas Kamba. Data yang diperoleh dianalisis dengan menggunakan Analisis Ragam (ANOVA) (P<0,05) dan uji lanjut Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa tepung sorghum terfermentasi terbaik didapatkan pada perlakuan lama fermentasi 72 jam dengan jumlah starter sebanyak 5%. Karakterisitik tepung sorgum ynag dimiliki adalah kadar pati 74,8%, kadar air 10,97%, swelling power 15,44 g/g, daya serap air 67,58% dan densitas kamba 0,6 g/ml. Modifikasi dengan fermentasi menggunakan yeast indigenous sorgum mampu memperbaiki sifat fisik dan kimia tepung sorgum. Berdasarkan kandungan gizi pada tepung sorgum telah memenuhi syarat mutu Codex Standard 173-1989 pada kadar, abu, protein, lemak, serat kasar dan karbohidrat.Kata kunci: sorgum, modifikasi, yeast indigenous, sifat fisikokimia

Study of C/N ratio of organic materials and its application in the production of natural fertilizer (bokashi)

Bokashi is an organic fertilizer made from organic materials through fermentation process. A high quality bokashi should have C/N ratio between 10-20, which normally produced from a mix raw organic material with has C/N ratio of 30. However, many studies have shown a composition of raw organic material without a calculation of C/N ratio. Therefore, the objectives of this study were to gather the initial C/N ratio of various raw material from previous published studies, calculate the C/N ratio, and examine the descriptive characteristic (colour, aroma, and texture) of bokashi made from three selected C/N ratios of raw material (30, 70, and 110) and various length of fermentation (10, 17, and 24 days). This research combined literature reviews and laboratory experimental. For laboratory experimental, Complete Randomise Design (CRD) was applied. The result showed that all bokashi produced from raw material mixture with the C/N ratio up to 110 was still acceptable to be directly applied as a fertilizer. Bokashi produced with longer fermentation time had better texture but lower aroma and colour quality.

Optimization of Small Peptide Feed from Milk Thistle Residue by Synergistic Fermentation of Multiple Strains and Proteases

In order to improve the utilization rate of the milk thistle residue, this study used the synergistic fermentation of multiple strains and proteases to increase the small peptide content of the fermented feed produced by the milk thistle residue. Taking the small peptide content of the milk thistle residue fermented feed as an indicator, the optimal fermentation process was obtained by single-factor optimization experiments and the response surface methodology. The optimal fermentation process was as follows: fermentation time of 7 days, inoculum size of 15%, inoculation ratio of aerobic strains: anaerobic strains = 1: 2, solid-state fermentation water content of 66%, fermentation temperature of 36℃, and amount of protease was 0.25% acid protease+0.25% bromelain. Under the above process, the small peptide content of the fermented feed from milk thistle residue was greatly improved to 57.86%. These results inferred that the added proteases were beneficial to the growth of fermentative microorganisms, the secretion of protease and the increase of the small peptide content.

Quality Analysis of Yoghurt from Goat's Milk Using Starter Lactic Acid Bacteria

Yoghurt is a pro-biotic beverage produced from the fermentation process of milk, namely from vegetable milk (soy milk) or animal milk (goat's milk and cow's milk). During the fermentation process, the chemical reactions that occur will turn milk into yogurt with the help of lactic acid bacteria. In the health sector, yogurt plays a role in increasing the body's immunity, digestive tract health and can prevent osteoporosis. In general, yogurt circulating in the community still has low nutritional content, so a more in-depth study needs to be done. The goal of this research is to examine the quality of yogurt by manipulating certain variables and adding other components to increase the yogurt's quality. Streptococcus thermophilus and Lactobacillus bulgaricus were used as starter as much as 12.5 mL each (10% of 500 mL of cream milk). The fixed variables in this study were the volume of goat's milk 500 ml, cream concentration 6%, pasteurization temperature 85°C, pasteurization time 15 minutes and fermentation temperature 45°C, while the independent variables were varying the length of the fermentation process for 3, 4, 5, 6, 7, 8 and 9 hours. From this study, it was concluded that yogurt with optimum results was obtained at a fermentation time of 6 hours, the pH value was 3.8, lactic acid content was 1.305%, protein content was 5.54%, fat content was 4.98%, and moisture content was 84.10%.

Technological innovation in the winery addressing oenology 4.0: Testing of an automated system for the alcoholic fermentation management

In recent years, the use of automated machine tools in the wine industry has increasingly gained ground to simplify and optimize winemaking, complying with Industry 4.0 requirements. This work aimed to analyse a system for the automatic management of yeast nutrition in alcoholic fermentation in terms of environmental, management, and economic performance in comparison with traditional fermentation management. The automated system is a transportable and easily installable place and start system, equipped with a control unit and rods for the dosage of nutrients, and it works with a memory unit in which fermentative kinetics curves are loaded. The curves are predefined or customized according to oenologists’ needs. Hence, fermentation time, manpower, nutrients, oxygen, water, and energy consumption were evaluated concerning the alcoholic fermentation process. The analysis was carried out considering two different Italian wineries with different working capacities. Furthermore, life cycle assessment methodology and variable costs analysis was performed. Overall, the automated system reveals to be a promising investment, especially if applied to wineries characterized by high-volume tanks, where scale factor played a crucial role. Nutrients used by the automated system are more expensive but more environmentally sustainable than traditional ones.

Optimization of pigment production by Rhodotorula minuta URM 5197 and Rhodotorula mucilaginosa URM 7409 using yellow passion fruit peel (Passiflora edulis)

The objective of this work was to optimize the production of pigments by Rhodotorula minuta and Rhodotorula mucilaginosa through submerged fermentation, using yellow passion fruit peel (Passiflora edulis), as the only substrate. The independent variables evaluated to optimize were: yellow passion fruit peel (YPFP), in grams (g) as culture medium, pH and fermentation time, in days. The study of pigment production and its optimization was carried out using the Doehlert matrix, with fifteen experimental conditions, of which thirteen had different combinations and two repeated the central point. The fixed variables were 30ºC and 150 rpm. Data analysis was performed using the Statistica software version 10.0. The largest amount of total pigments and total carotenoids produced by R. minuta was 28±0.01 mg/L and 72.8±0.026 µg/g, respectively; while for R. mucilaginosa the production of total pigments was 37±0.002 mg/L and total carotenoids 236.8±0.013 µg/g. The optimum production point of total pigments for R. minuta was 2.3g of YPFP, pH 6.5 and 5 days and for R. mucilaginosa 2.5g of YPFP pH 6 and 5 days. In samples of total pigments, the presence of 0.29mg/L of β-carotene for R. minuta and 0.83 mg/L for R. mucilaginosa was identified. It is possible to conclude that yellow passion fruit peel can be used as a nutrient source for Rhodotorula spp growth and pigment production with total carotenoids and β-carotene in its composition.