Impact of sweet sorghum cuticular waxes (SSCW) on acetone–butanol–ethanol fermentation using Clostridium acetobutylicum ABE1201

2013 ◽  
Vol 149 ◽  
pp. 470-473 ◽  
Author(s):  
Di Cai ◽  
Zhen Chang ◽  
Chengyu Wang ◽  
Wenqiang Ren ◽  
Zheng Wang ◽  
...  
Keyword(s):  
Ethanol Fermentation ◽  
Clostridium Acetobutylicum ◽  
Sweet Sorghum ◽  
Cuticular Waxes ◽  
Acetone Butanol Ethanol

Targeted mutagenesis of the Clostridium acetobutylicum acetone–butanol–ethanol fermentation pathway

2012 ◽  
Vol 14 (6) ◽  
pp. 630-641 ◽  
Author(s):  
Clare M. Cooksley ◽  
Ying Zhang ◽  
Hengzheng Wang ◽  
Stephanie Redl ◽  
Klaus Winzer ◽  
...  
Keyword(s):  
Ethanol Fermentation ◽  
Clostridium Acetobutylicum ◽  
Targeted Mutagenesis ◽  
Acetone Butanol Ethanol

scholarly journals Formic Acid Triggers the “Acid Crash” of Acetone-Butanol-Ethanol Fermentation byClostridium acetobutylicum

2011 ◽  
Vol 77 (5) ◽  
pp. 1674-1680 ◽  
Author(s):  
Shaohua Wang ◽  
Yanping Zhang ◽  
Hongjun Dong ◽  
Shaoming Mao ◽  
Yan Zhu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Formic Acid ◽  
Butyric Acid ◽  
Ethanol Fermentation ◽  
Clostridium Acetobutylicum ◽  
Recombinant Strain ◽  
Formate Dehydrogenase ◽  
Glucose Medium ◽  
Empty Plasmid ◽  
Acetone Butanol Ethanol

ABSTRACTSolvent production byClostridium acetobutylicumcollapses when cells are grown in pH-uncontrolled glucose medium, the so-called “acid crash” phenomenon. It is generally accepted that the fast accumulation of acetic acid and butyric acid triggers the acid crash. We found that addition of 1 mM formic acid into corn mash medium could trigger acid crash, suggesting that formic acid might be related to acid crash. When it was grown in pH-uncontrolled glucose medium or glucose-rich medium,C. acetobutylicumDSM 1731 containing the empty plasmid pIMP1 failed to produce solvents and was found to accumulate 0.5 to 1.24 mM formic acid intracellularly. In contrast, recombinant strain DSM 1731 with formate dehydrogenase activity did not accumulate formic acid intracellularly and could produce solvent as usual. We therefore conclude that the accumulation of formic acid, rather than acetic acid and butyric acid, is responsible for the acid crash of acetone-butanol-ethanol fermentation.

CaCO3 supplementation alleviates the inhibition of formic acid on acetone/butanol/ethanol fermentation by Clostridium acetobutylicum

2016 ◽  
Vol 39 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Gaoxiang Qi ◽  
Lian Xiong ◽  
Xiaoqing Lin ◽  
Chao Huang ◽  
Hailong Li ◽  
...  
Keyword(s):  
Formic Acid ◽  
Ethanol Fermentation ◽  
Clostridium Acetobutylicum ◽  
Acetone Butanol Ethanol
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