Anomalously high aggregation level of the polyene antibiotic amphotericin B in acidic medium: Implications for the biological action

2008 ◽  
Vol 136 (1) ◽  
pp. 44-49 ◽  
Author(s):  
Mariusz Gagoś ◽  
Monika Hereć ◽  
Marta Arczewska ◽  
Grzegorz Czernel ◽  
Mauro Dalla Serra ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Acidic Medium ◽  
Biological Action ◽  
Polyene Antibiotic ◽  
Aggregation Level ◽  
High Aggregation Level

scholarly journals Changes in erythrocyte membrane permeability induced by verapamil, chlorpromazine, and their combinations with amphotericin B

2011 ◽  
Vol 48 (2) ◽  
pp. 225-241 ◽  
Author(s):  
Agnieszka Knopik-Skrocka ◽  
Józef Bielawski ◽  
Szymon Chowański ◽  
Paulina Walkowiak
Keyword(s):  
Membrane Permeability ◽  
Amphotericin B ◽  
Erythrocyte Membrane ◽  
Hemolytic Activity ◽  
Divalent Cations ◽  
Polyene Antibiotic ◽  
Erythrocyte Suspension ◽  
Sucrose Medium ◽  
Erythrocyte Membrane Permeability ◽  
Evident Difference

Changes in erythrocyte membrane permeability induced by verapamil, chlorpromazine, and their combinations with amphotericin BHemolysis induced by 2 amphipathic agents, verapamil and chlorpromazine, was investigated in various incubation conditions. Changes in absorbance of erythrocyte suspension were monitored by absorption spectrophotometry at a wavelength of 590 nm. The hemolysis induced by verapamil or chlorpromazine is of the permeability type. The resistance of erythrocytes to verapamil is much higher than their resistance to chlorpromazine. No evident difference is found between human and pig erythrocytes in their resistance to verapamil. Only a small decrease in the rate of hemolysis induced by verapamil is observed in isotonic CaCl2, MgCl2or K2SO4solutions, compared to 160 mM KCl (the standard incubation medium). The changes in hemolytic activity of chlorpromazine in the presence of the divalent cations and anions are less evident. No decrease in hemolytic activity of chlorpromazine and verapamil is observed in the sucrose medium. The hemolytic activity of both the agents increases when they act in combination with polyene antibiotic amphotericin B. The results indicate a strong synergy between amphotericin B and verapamil or chlorpromazine. By contrast, a combined effect of verapamil and chlorpromazine on erythrocytes leads to a decrease in their hemolytic activity. This indicates antagonism between verapamil and chlorpromazine.

scholarly journals Oligonucleotide delivery by a cationic derivative of the polyene antibiotic amphotericin B

2000 ◽  
Vol 1464 (2) ◽  
pp. 299-308 ◽  
Author(s):  
Isabelle Blanc ◽  
Maria-Helena Bueno Da Costa ◽  
Jacques Bolard ◽  
Michèle Saint-Pierre Chazalet
Keyword(s):  
Amphotericin B ◽  
Polyene Antibiotic ◽  
Oligonucleotide Delivery

Kinetics of a rapid, luminol dependent chemiluminescence signal induced in HL-60 cells by amphotericin B and other stimulants

1991 ◽  
Vol 69 (9) ◽  
pp. 618-623 ◽  
Author(s):  
D. A. O'Keefe ◽  
D. R. James ◽  
W. R. Ware ◽  
N. O. Petersen
Keyword(s):  
Tissue Culture ◽  
Superoxide Dismutase ◽  
Amphotericin B ◽  
Culture Medium ◽  
Polyene Antibiotic ◽  
Biochemical Processes ◽  
Rate Determining Step ◽  
Rate Limiting ◽  
Kinetics Of ◽  
Peak Value

Addition of the polyene antibiotic amphotericin B or tissue culture medium to nondifferentiated HL-60 cells in the presence of luminol induces a chemiluminescence signal that reaches a peak value within a few seconds and decays exponentially in less than a minute. The kinetics of the signal and its modulation by superoxide dismutase, catalase, and horseradish peroxidase are consistent with a series of solution biochemical processes with a rate-determining step corresponding to the disproportionation of a luminal–superoxide complex. The effects of the enzymes demonstrate that superoxide is a precursor to the rate-determining intermediate and that both catalase and peroxide enhance a reaction that competes with the rate-limiting process.Key words: chemiluminescence, luminol, amphotericin B, superoxide, HL-60 cells.

The Methodology of National Innovation System Analysis

2015 ◽  
pp. 592-621
Author(s):  
Oleg Golichenko
Keyword(s):  
Market Failure ◽  
System Analysis ◽  
Innovation System ◽  
Business Environment ◽  
National Innovation System ◽  
System Structure ◽  
New Knowledge ◽  
Key Policy ◽  
Aggregation Level ◽  
High Aggregation Level

Many efforts have been made in developing the National Innovation System (NIS) concept. However, there are the limitations, which do not make it operable and effective in practice. This investigation attempts to eliminate some limitations of the approach. The NIS is presented as three interrelated macro blocs: business environment, environment producing new knowledge, and knowledge transfer mechanism. The principles of performance and efficiency measuring of NIS are proposed. The system structure-object and functional approaches to NIS performance and efficiency are applied. The former is used for decomposition of NIS objects of high aggregation level. The latter is available for analysis of NIS efficiency and its factors. The methods allow the estimation of the NIS component by component and identification of the cause-effect chains of factor impacts on its elements. The key policy tools tailored to liquidate and mitigate market failure and NIS dysfunctions are proposed.

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