The role of fluorescence in situ hybridization (FISH) for monitoring hematologic malignancies with BCR/ABL or ETO/AML1 rearrangement: a comparative study with FISH and G-banding on 919 consecutive specimens of hematologic malignancies

Rearrangements within the chromosome 11q13 region are frequent in hematologic malignancies. 50% of 75% of mantle cell lymphomas (MCLs) carry a translocation t(11;14) (q13;q32). Using Southern blot analysis, a BCL1 breakpoint can be detected in approximately 50% of MCLs. It is not known whether other MCLs harbor also breakpoints at 11q13. Breakpoints in this region not involved in t(11;14), are detected in chronic lymphocytic leukemia and acute myeloid leukemia. To detect and localize breakpoints at 11q13 more accurately, we have developed fluorescence in situ hybridization using two probe sets of differently labeled cosmids, symmetrically localized at either side of the major translocation cluster of BCL1. These probes span a region of 450 to 750 kb. We applied this assay to a series of hematologic malignancies with 11q13 abnormalities identified by classical cytogenetics. All four samples with a t(11;14) (q13;q32) showed dissociation of the differently colored signals in metaphase and interphase cells, thereby indicating a chromosomal break in the region defined by the probe sets. The frequency of abnormal metaphase and interphase cells was comparable with that observed in any of the 13 malignancies with other chromosomal 11q13 abnormalities, indicating that these chromosomal breaks occurred outside the 450- to 750-kb region covered by the probes. One patient showed triplication and one patient showed monoallelic loss of this region. The current data show that double-color fluorescence in situ hybridization is a simple and reliable method for detection of the t(11;14)(q13;q32) in interphase cell nuclei and that is can be used to distinguish this translocation from other 11q13 rearrangements in hematologic malignancies.

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Fluorescence in situ hybridization analyses of hematologic malignancies reveal frequent cytogenetically unrecognized 12p rearrangements

Leukemia ◽

10.1038/sj.leu.2400929 ◽

1998 ◽

Vol 12 (3) ◽

pp. 390-400 ◽

Cited By ~ 35

Author(s):

P Andreasson ◽

B Johansson ◽

R Billström ◽

S Garwicz ◽

F Mitelman ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Hematologic Malignancies

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Discrepancies between immunohistochemistry and fluorescence in situ hybridization for HER-2 testing in breast cancer: The role of a quality assurance program on a cohort of 3564 patients

Journal of Clinical Oncology ◽

10.1200/jco.2004.22.14_suppl.679 ◽

2004 ◽

Vol 22 (14_suppl) ◽

pp. 679-679 ◽

Cited By ~ 1

Author(s):

H. Yaziji ◽

T. S. Barry ◽

L. C. Goldstein ◽

H. H. Hwang ◽

A. W. Hing ◽

...

Keyword(s):

Breast Cancer ◽

Quality Assurance ◽

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Quality Assurance Program ◽

Her 2

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The role of fluorescence in situ hybridization technologies in molecular diagnostics and disease management

Molecular Diagnosis ◽

10.1054/modi.2000.19213 ◽

2000 ◽

Vol 5 (4) ◽

pp. 309-319 ◽

Cited By ~ 2

Author(s):

W King

Keyword(s):

In Situ Hybridization ◽

Disease Management ◽

Fluorescence In Situ Hybridization ◽

Molecular Diagnostics

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Comparative Study between Quantitative Digital Image Analysis and Fluorescence In Situ Hybridization of Breast Cancer Equivocal HER2 2+ Cases

Analytical Cellular Pathology ◽

10.1155/2014/608254 ◽

2014 ◽

Vol 2014 ◽

pp. 1-1

Author(s):

E. Ayad ◽

M. Mansy ◽

D. Elwi ◽

M. Salem ◽

M. Salama ◽

...

Keyword(s):

Breast Cancer ◽

Image Analysis ◽

In Situ Hybridization ◽

Comparative Study ◽

Fluorescence In Situ Hybridization ◽

Digital Image ◽

Digital Image Analysis

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Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity

Comparative Cytogenetics ◽

10.3897/compcytogen.v15.i2.60649 ◽

2021 ◽

Vol 15 (2) ◽

pp. 119-136

Author(s):

Pun Yeesin ◽

Phichaya Buasriyot ◽

Sukhonthip Ditcharoen ◽

Patcharaporn Chaiyasan ◽

Chatmongkon Suwannapoom ◽

...

Keyword(s):

In Situ Hybridization ◽

Comparative Study ◽

Fluorescence In Situ Hybridization ◽

Chromosome Numbers ◽

Single Pair ◽

Molecular Cytogenetic ◽

Karyotypic Diversity ◽

Species Specific

Karyotypes of four catfishes of the genus Mystus Scopoli, 1777 (family Bagridae), M. atrifasciatus Fowler, 1937, M. mysticetus Roberts, 1992, M. singaringan (Bleeker, 1846) and M. wolffii (Bleeker, 1851), were analysed by conventional and Ag-NOR banding as well as fluorescence in situ hybridization (FISH) techniques. Microsatellite d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeat probes were applied in FISH. The obtained data revealed that the four studied species have different chromosome complements. The diploid chromosome numbers (2n) and the fundamental numbers (NF) range between 52 and 102, 54 and 104, 56 and 98, or 58 and 108 in M. mysticetus, M. atrifasciatus, M. singaringan or M. wolffii, respectively. Karyotype formulae of M. mysticetus, M. atrifasciatus, M. singaringan and M. wolffii are 24m+26sm+4a, 26m+24sm+2a, 24m+18sm+14a and 30m+22sm+6a, respectively. A single pair of NORs was identified adjacent to the telomeres of the short arm of chromosome pairs 3 (metacentric) in M. atrifasciatus, 20 (submetacentric) in M. mysticetus, 15 (submetacentric) in M. singaringan, and 5 (metacentric) in M. wolffii. The d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeats were abundantly distributed in species-specific patterns. Overall, we present a comparison of cytogenetic and molecular cytogenetic patterns of four species from genus Mystus providing insights into their karyotype diversity in the genus.

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