scholarly journals Selective isolation of β-glucan from corn pericarp hemicelluloses by affinity chromatography on cellulose column

2014 ◽  
Vol 111 ◽  
pp. 538-542 ◽  
Author(s):  
Tomoki Yoshida ◽  
Yoichi Honda ◽  
Takashi Tsujimoto ◽  
Hiroshi Uyama ◽  
Jun-ichi Azuma
Keyword(s):  
Affinity Chromatography ◽  
Selective Isolation ◽  
Cellulose Column

The Use of Lectin Affinity Chromatography for the Selective Isolation of Plasma Membranes

1984 ◽  
Vol 14 (2) ◽  
pp. 149-161 ◽  
Author(s):  
L. J. Kaplan ◽  
F. G. Barr ◽  
M. Daims ◽  
D. Nelson ◽  
T. B. Tanner
Keyword(s):  
Affinity Chromatography ◽  
Plasma Membranes ◽  
Selective Isolation ◽  
Lectin Affinity Chromatography ◽  
Lectin Affinity

The Isolation of Chymotrypsin-Like Enzymes by Affinity Chromatography Using Sepharose–4-Phenyibutyiamine

1971 ◽  
Vol 49 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Kenneth J. Stevenson ◽  
Amiram Landman
Keyword(s):  
Affinity Chromatography ◽  
Active Site ◽  
Alces Alces ◽  
Alkaline Solutions ◽  
Selective Isolation ◽  
Weakly Alkaline ◽  
Irreversible Inhibitors ◽  
Bovine Trypsin ◽  
Pass Through

Affinity chromatography of chymotrypsin-like proteases on a column of Sepharose–4-phenylbutylamine (PBA) has been developed. Sepharose–PBA (Sepharose–NH∙[CH2]4∙C6H5) has been shown to selectively adsorb chymotrypsin α and B from weakly alkaline solutions and to allow to pass through unretarded porcine trypsin, bovine trypsinogen, and chymotrypsin α modified with active-site-directed irreversible inhibitors. Chymotrypsinogen A and bovine trypsin were only slightly retarded whereas a preparation of subtilisin was markedly retarded and separated into two distinct peaks. Sepharose–PBA has been utilized successfully for the selective isolation of chymotryps in-like proteases from extracts of moose pancreas (Alces alces).

Selective isolation of G-quadruplexes by affinity chromatography

2012 ◽  
Vol 1246 ◽  
pp. 62-68 ◽  
Author(s):  
Tianjun Chang ◽  
Xiangjun Liu ◽  
Xiaohong Cheng ◽  
Cui Qi ◽  
Hongcheng Mei ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Selective Isolation

Selective isolation of mercurated DNA by affinity chromatography on thiol matrices

1985 ◽  
Vol 146 (1) ◽  
pp. 64-70 ◽  
Author(s):  
Gaspar Banfalvi ◽  
Sudha Bhattacharya ◽  
Nilima Sarkar
Keyword(s):  
Affinity Chromatography ◽  
Selective Isolation

The microstructure of functionalized poLyacrylonitrile beads for bioseparations

1990 ◽  
Vol 48 (4) ◽  
pp. 1094-1095
Author(s):  
Eduardo A. Kamenetzky ◽  
David A. Ley
Keyword(s):  
Aqueous Solution ◽  
Affinity Chromatography ◽  
Pore Size Distribution ◽  
Pore Size ◽  
Surface Coverage ◽  
Vacuum Impregnation ◽  
Thin Sections ◽  
Selective Staining ◽  
Low Viscosity ◽  
Diamond Knife

The microstructure of polyacrylonitrile (PAN) beads for affinity chromatography bioseparations was studied by TEM of stained ultramicrotomed thin-sections. Microstructural aspects such as overall pore size distribution, the distribution of pores within the beads, and surface coverage of functionalized beads affect performance properties. Stereological methods are used to quantify the internal structure of these chromatographic supports. Details of the process for making the PAN beads are given elsewhere. TEM specimens were obtained by vacuum impregnation with a low-viscosity epoxy and sectioning with a diamond knife. The beads can be observed unstained. However, different surface functionalities can be made evident by selective staining. Amide surface coverage was studied by staining in vapor of a 0.5.% RuO4 aqueous solution for 1 h. RuO4 does not stain PAN but stains, amongst many others, polymers containing an amide moiety.

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