Affinity Chromatography on Immobilized Anhydrotrypsin: General Utility for Selective Isolation of C-Terminal Peptides from Protease Digests of Proteins1

1987 ◽  
Vol 102 (6) ◽  
pp. 1539-1546 ◽  
Author(s):  
Takashi KUMAZAKI ◽  
Kumiko TERASAWA ◽  
Shin-ichi ISHII
Keyword(s):  
Affinity Chromatography ◽  
Selective Isolation ◽  
General Utility

The Use of Lectin Affinity Chromatography for the Selective Isolation of Plasma Membranes

1984 ◽  
Vol 14 (2) ◽  
pp. 149-161 ◽  
Author(s):  
L. J. Kaplan ◽  
F. G. Barr ◽  
M. Daims ◽  
D. Nelson ◽  
T. B. Tanner
Keyword(s):  
Affinity Chromatography ◽  
Plasma Membranes ◽  
Selective Isolation ◽  
Lectin Affinity Chromatography ◽  
Lectin Affinity

The Isolation of Chymotrypsin-Like Enzymes by Affinity Chromatography Using Sepharose–4-Phenyibutyiamine

1971 ◽  
Vol 49 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Kenneth J. Stevenson ◽  
Amiram Landman
Keyword(s):  
Affinity Chromatography ◽  
Active Site ◽  
Alces Alces ◽  
Alkaline Solutions ◽  
Selective Isolation ◽  
Weakly Alkaline ◽  
Irreversible Inhibitors ◽  
Bovine Trypsin ◽  
Pass Through

Affinity chromatography of chymotrypsin-like proteases on a column of Sepharose–4-phenylbutylamine (PBA) has been developed. Sepharose–PBA (Sepharose–NH∙[CH2]4∙C6H5) has been shown to selectively adsorb chymotrypsin α and B from weakly alkaline solutions and to allow to pass through unretarded porcine trypsin, bovine trypsinogen, and chymotrypsin α modified with active-site-directed irreversible inhibitors. Chymotrypsinogen A and bovine trypsin were only slightly retarded whereas a preparation of subtilisin was markedly retarded and separated into two distinct peaks. Sepharose–PBA has been utilized successfully for the selective isolation of chymotryps in-like proteases from extracts of moose pancreas (Alces alces).

scholarly journals Selective isolation of β-glucan from corn pericarp hemicelluloses by affinity chromatography on cellulose column

2014 ◽  
Vol 111 ◽  
pp. 538-542 ◽  
Author(s):  
Tomoki Yoshida ◽  
Yoichi Honda ◽  
Takashi Tsujimoto ◽  
Hiroshi Uyama ◽  
Jun-ichi Azuma
Keyword(s):  
Affinity Chromatography ◽  
Selective Isolation ◽  
Cellulose Column

Selective isolation of G-quadruplexes by affinity chromatography

2012 ◽  
Vol 1246 ◽  
pp. 62-68 ◽  
Author(s):  
Tianjun Chang ◽  
Xiangjun Liu ◽  
Xiaohong Cheng ◽  
Cui Qi ◽  
Hongcheng Mei ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Selective Isolation

Selective isolation of mercurated DNA by affinity chromatography on thiol matrices

1985 ◽  
Vol 146 (1) ◽  
pp. 64-70 ◽  
Author(s):  
Gaspar Banfalvi ◽  
Sudha Bhattacharya ◽  
Nilima Sarkar
Keyword(s):  
Affinity Chromatography ◽  
Selective Isolation

Microstructure of laser-irradiated, conducting Kapton polyimide

1995 ◽  
Vol 53 ◽  
pp. 502-503
Author(s):  
D. L. Callahan ◽  
Z. Ball ◽  
H. M. Phillips ◽  
R. Sauerbrey
Keyword(s):  
Electron Microscopy ◽  
Phase Transition ◽  
Transmission Electron Microscopy ◽  
Cross Section ◽  
Film Surface ◽  
Cross Sectional ◽  
General Utility ◽  
Transmission Electron ◽  
Considerable Debate ◽  
Potential Applications

Ultraviolet laser-irradiation can be used to induce an insulator-to-conductor phase transition on the surface of Kapton polyimide. Such structures have potential applications as resistors or conductors for VLSI applications as well as general utility electrodes. Although the percolative nature of the phase transformation has been well-established, there has been little definitive work on the mechanism or extent of transformation. In particular, there has been considerable debate about whether or not the transition is primarily photothermal in nature, as we propose, or photochemical. In this study, cross-sectional optical microscopy and transmission electron microscopy are utilized to characterize the nature of microstructural changes associated with the laser-induced pyrolysis of polyimide.Laser-modified polyimide samples initially 12 μm thick were prepared in cross-section by standard ultramicrotomy. Resulting contraction in parallel to the film surface has led to distortions in apparent magnification. The scale bars shown are calibrated for the direction normal to the film surface only.

The microstructure of functionalized poLyacrylonitrile beads for bioseparations

1990 ◽  
Vol 48 (4) ◽  
pp. 1094-1095
Author(s):  
Eduardo A. Kamenetzky ◽  
David A. Ley
Keyword(s):  
Aqueous Solution ◽  
Affinity Chromatography ◽  
Pore Size Distribution ◽  
Pore Size ◽  
Surface Coverage ◽  
Vacuum Impregnation ◽  
Thin Sections ◽  
Selective Staining ◽  
Low Viscosity ◽  
Diamond Knife

The microstructure of polyacrylonitrile (PAN) beads for affinity chromatography bioseparations was studied by TEM of stained ultramicrotomed thin-sections. Microstructural aspects such as overall pore size distribution, the distribution of pores within the beads, and surface coverage of functionalized beads affect performance properties. Stereological methods are used to quantify the internal structure of these chromatographic supports. Details of the process for making the PAN beads are given elsewhere. TEM specimens were obtained by vacuum impregnation with a low-viscosity epoxy and sectioning with a diamond knife. The beads can be observed unstained. However, different surface functionalities can be made evident by selective staining. Amide surface coverage was studied by staining in vapor of a 0.5.% RuO4 aqueous solution for 1 h. RuO4 does not stain PAN but stains, amongst many others, polymers containing an amide moiety.

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