Effects of environmental anoxia and different periods of reoxygenation on oxidative balance in gills of the estuarine crab Chasmagnathus granulata

2005 ◽  
Vol 140 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Ubirajara O. de Oliveira ◽  
Alex Sander da Rosa Araújo ◽  
Adriane Belló-Klein ◽  
Roselis S.M. da Silva ◽  
Luiz Carlos Kucharski
Keyword(s):  
Oxidative Balance ◽  
Chasmagnathus Granulata ◽  
Crab Chasmagnathus Granulata

Oxidative balance and immunodetection of antioxidant enzymes in the hepatopancreas of the crab Chasmagnathus granulata subjected to anoxia and reoxygenation

2006 ◽  
Vol 84 (5) ◽  
pp. 677-684 ◽  
Author(s):  
Ubirajara O. de Oliveira ◽  
Adriane Belló-Klein ◽  
Luiz Carlos Kucharski
Keyword(s):  
Antioxidant Enzymes ◽  
Thiobarbituric Acid ◽  
Glutathione S Transferase ◽  
Defense Systems ◽  
Oxidative Balance ◽  
Chasmagnathus Granulata ◽  
Antioxidant Defense Systems ◽  
Response Profiles ◽  
Different Response ◽  
Crab Chasmagnathus Granulata

The present study shows the activities and concentrations of the antioxidant enzymes superoxide dismutase (SOD) and glutathione S-transferase (GST) in the hepatopancreas of the crab Chasmagnathus granulata (Dana, 1851) (Decapoda, Brachyura) when exposed to periods of anoxia (8 h) and aerobic recovery (20 and 40 min post anoxia). Exposure to anoxia did not cause any change in the activities of the enzymes analyzed. The activities of these enzymes did not change with a decrease in environmental oxygen concentration. During reoxygenation, enzyme activities returned to control levels. The concentrations and activities of SOD and GST presented different response profiles. Exposure to anoxia caused increased lipoperoxidation (conjugated dienes and thiobarbituric acid reactive substances), whereas aerobic recovery reduced lipoperoxidation. The results of this study showed that C. granulata adjusts its antioxidant defense systems in an attempt to reduce and (or) avoid damage resulting from the reintroduction of oxygen.

Antioxidant Defenses and DNA Damage Induced by UV-A and UV-B Radiation in the Crab Chasmagnathus granulata (Decapoda, Brachyura)¶

2005 ◽  
Vol 81 (2) ◽  
pp. 398 ◽  
Author(s):  
Glauce R. Gouveia ◽  
Daiane S. Marques ◽  
Bruno P. Cruz ◽  
Laura A. Geracitano ◽  
Luiz E. M. Nery ◽  
...  
Keyword(s):  
Dna Damage ◽  
Antioxidant Defenses ◽  
Chasmagnathus Granulata ◽  
Crab Chasmagnathus Granulata

scholarly journals Anticholinesterase effect of eserine (physostigmine) in fish and crustacean species

2001 ◽  
Vol 44 (1) ◽  
pp. 63-68 ◽  
Author(s):  
José M. Monserrat ◽  
Adalto Bianchini
Keyword(s):  
Cholinesterase Activity ◽  
Fish Larvae ◽  
Kinetic Characteristic ◽  
Natural Substrate ◽  
Crustacean Species ◽  
Lower Affinity ◽  
Substrate Analogues ◽  
Farfantepenaeus Paulensis ◽  
Chasmagnathus Granulata ◽  
Crab Chasmagnathus Granulata

The kinetic characteristic (Km) of cholinesterase from the crab Chasmagnathus granulata, the shrimp Farfantepenaeus paulensis and the fish Odontesthes bonaeriensis were compared and correlated with the anticholinesterasic effect of eserine (physostigmine). For the crustaceans, the estimated Km values were about 5-8 times higher than that estimated for the fish (0.04 mM). In the crab and the shrimp, the concentration of eserine which inhibited 50% of cholinesterase activity (IC50) was estimated as 5.33x10-4 and 4.33x10-4 mM, respectively. In both cases, it was significantly higher (P < 0.05) than that estimated for the fish larvae (7.43x10-5 mM). A high Km could reflect a lower affinity of the cholinesterase for its natural substrate, acetylcholine, or for substrate analogues such as carbamates and organophosphorous pesticides. If we consider the IC50 for eserine as an index of enzyme susceptibility to pesticide inhibition, the cholinesterase from the fish larvae may be a better useful tool in assays for pesticide biomonitoring than that from crustacean species.

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