Induction of cytochrome P4501A by highly purified hexachlorobenzene in primary cultures of ring-necked pheasant and Japanese quail embryo hepatocytes

Author(s):  
Lukas J. Mundy ◽  
Doug Crump ◽  
Stephanie P. Jones ◽  
Alex Konstantinov ◽  
Fiona Utley ◽  
...  
1974 ◽  
Vol 27 (1) ◽  
pp. 218-223 ◽  
Author(s):  
W. M. Colwell ◽  
D. G. Simmons ◽  
K. E. Muse

1974 ◽  
Vol 27 (1) ◽  
pp. 218-223
Author(s):  
W. M. Colwell ◽  
D. G. Simmons ◽  
K. E. Muse

2001 ◽  
Vol 29 (3) ◽  
pp. 251-257 ◽  
Author(s):  
Helmut Segner ◽  
Jean-Pierre Cravedi

In aquatic toxicology, isolated liver cells from fish can be used as a tool to generate initial information on the hepatic metabolism of xenobiotics, and on the mechanisms of xenobiotic activation or deactivation. This isolation of teleost liver cells is achieved by enzymic dissociation, and monolayer cultures of fish hepatocytes in serum-free medium maintain good viability for 3–8 days. During in vitro culture, fish liver cells express stable levels of phase I and phase II enzymes, such as cytochrome P4501A or glutathione S-transferase, and the cells show an induction of biotransformation enzymes after exposure to xenobiotics. The xenobiotic metabolite pattern produced by fish hepatocytes in vitro is generally similar to that observed in vivo. Limitations to more-intensive application of cultured fish hepatocytes as a screen in aquatic hazard assessment are partly due to the rather limited scope of existing studies, i.e. the focus on one particular species (rainbow trout), and on one particular biotransformation enzyme (cytochrome P4501A), as well as a lack of comparative in vitro/in vivo studies.


2020 ◽  
Vol 66 (1) ◽  
pp. 115-123
Author(s):  
Saad El-Shater ◽  
Karim Khalil ◽  
Hamdy Rizk ◽  
Hisham Abdelrahman ◽  
Elsayed Khalifa

2004 ◽  
Vol 17 (4) ◽  
pp. 245-252 ◽  
Author(s):  
Kazumoto Shibuya ◽  
Makoto Mizutani ◽  
Masaru Wada ◽  
Kazuo Sato ◽  
Tetsuo Nunoya

1973 ◽  
Vol 17 (3) ◽  
pp. 199-203 ◽  
Author(s):  
Naohide Takayama ◽  
Bunsiti Simizu

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