Improved production of 1,3-propanediol from biodiesel-derived crude glycerol by Klebsiella pneumoniae in fed-batch fermentation

Nowadays, the utilization of the by-product glycerol has become a common problem with the large amount of biodiesel production. This paper studied the conversion of the by-product glycerol of biodiesel production by Klebsiella pneumonia to 1,3-propanediol. The crude glycerol could be used directly without refining by a lactate dehydrogenase deficient K. pneumoniae and the lactic acid concentration was very low. In the fed-batch fermentation of 7L fermenter, 93.3g/l 1,3-propanediol was obtained and lactic acid concentration was just 2g/l. Analysis showed that D-type lactic acid synthesis was reduced obviously. The fermentation also was scaled up on the 42L fermenter. 1,3-propanediol and 2,3-Butanediol concentration reached 81.5g/l and 33.8g/l, respectively. No lactic acid was detected. The demonstration fermentation in 5000L fermenter was also successfully performed. The final 1,3-propanediol concentration and productivity was 87g/l and 1.2g/(lh). This work was considered useful to the integrated production of biodiesel and 1,3-propanediol.

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Efficient production of 2,3-butanediol from cheese whey powder (CWP) solution by Klebsiella pneumoniae through integrating pulsed fed-batch fermentation with a two-stage pH control strategy

Fuel ◽

10.1016/j.fuel.2017.04.138 ◽

2017 ◽

Vol 203 ◽

pp. 469-477 ◽

Cited By ~ 7

Author(s):

Xuewu Guo ◽

Yazhou Wang ◽

Jian Guo ◽

Qing Wang ◽

Yu Zhang ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Control Strategy ◽

Batch Fermentation ◽

Cheese Whey ◽

Ph Control ◽

Efficient Production ◽

Fed Batch ◽

Two Stage ◽

Whey Powder ◽

Fed Batch Fermentation

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Enhance 1,3-propanediol production from crude glycerol in batch and fed-batch fermentation with two-phase pH-controlled strategy

Electronic Journal of Biotechnology ◽

10.2225/vol14-issue6-fulltext-6 ◽

2011 ◽

Vol 14 (6) ◽

Cited By ~ 1

Author(s):

Poonsuk Prasertsan ◽

Supalak Sattayasamitsathit ◽

Pawadee Methacanon

Keyword(s):

Batch Fermentation ◽

Crude Glycerol ◽

Fed Batch ◽

Two Phase ◽

Fed Batch Fermentation ◽

Ph Controlled

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A simple strategy to effectively produce d-lactate in crude glycerol-utilizing Escherichia coli

Biotechnology for Biofuels ◽

10.1186/s13068-019-1615-4 ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 2

Author(s):

Yao-De Wang ◽

Jin-Yi Liao ◽

Chung-Jen Chiang ◽

Yun-Peng Chao

Keyword(s):

Escherichia Coli ◽

Batch Fermentation ◽

Crude Glycerol ◽

High Titer ◽

Fed Batch ◽

High Productivity ◽

Simple Strategy ◽

Production Scheme ◽

Fed Batch Fermentation ◽

Fermentation Strategy

Abstract Background Fed-batch fermentation has been conventionally implemented for the production of lactic acid with a high titer and high productivity. However, its operation needs a complicated control which increases the production cost. Results This issue was addressed by simplifying the production scheme. Escherichia coli was manipulated for its glycerol dissimilation and d-lactate synthesis pathways and then subjected to adaptive evolution under high crude glycerol. Batch fermentation in the two-stage mode was performed by controlling the dissolved oxygen (DO), and the evolved strain deprived of poxB enabled production of 100 g/L d-lactate with productivity of 1.85 g/L/h. To increase productivity, the producer strain was further evolved to improve its growth rate on crude glycerol. The fermentation was performed to undergo the aerobic growth with low substrate, followed by the anaerobic production with high substrate. Moreover, the intracellular redox of the strain was balanced by fulfillment of the anaerobic respiratory chain with nitrate reduction. Without controlling the DO, the microbial fermentation resulted in the homofermentative production of d-lactate (ca. 0.97 g/g) with a titer of 115 g/L and productivity of 3.29 g/L/h. Conclusions The proposed fermentation strategy achieves the highest yield based on crude glycerol and a comparable titer and productivity as compared to the approach by fed-batch fermentation. It holds a promise to sustain the continued development of the crude glycerol-based biorefinery.

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