Efficient production of 2,3-butanediol from cheese whey powder (CWP) solution by Klebsiella pneumoniae through integrating pulsed fed-batch fermentation with a two-stage pH control strategy

Fuel ◽  
2017 ◽  
Vol 203 ◽  
pp. 469-477 ◽  
Author(s):  
Xuewu Guo ◽  
Yazhou Wang ◽  
Jian Guo ◽  
Qing Wang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Control Strategy ◽  
Batch Fermentation ◽  
Cheese Whey ◽  
Ph Control ◽  
Efficient Production ◽  
Fed Batch ◽  
Two Stage ◽  
Whey Powder ◽  
Fed Batch Fermentation

scholarly journals Efficient Production of 2,3-Butanediol from whey Powder by Metabolic Engineered Klebsiella Oxytoca

2020 ◽  
Author(s):  
Wensi Meng ◽  
Yongjia Zhang ◽  
Menghao Cao ◽  
Wen Zhang ◽  
Chuanjuan Lü ◽  
...  
Keyword(s):  
Environmental Pollution ◽  
Batch Fermentation ◽  
Klebsiella Oxytoca ◽  
Pollution Source ◽  
Efficient Production ◽  
Fermentable Sugar ◽  
Fed Batch ◽  
Whey Powder ◽  
Fed Batch Fermentation ◽  
Lactose Utilization

Abstract Backgrounds: Whey is the major pollution source from the dairy industry. Exploring new outlets for whey utilization is urgently needed to decline its environmental pollution. In this study, we explored the possibility of using whey powder to produce 2,3-butanediol (2,3-BD), an important platform chemical.Results: A Klebsiella oxytoca strain PDL-0 was selected from five 2,3-BD producing strains based on its ability to efficiently produce 2,3-BD from lactose, the major fermentable sugar in whey. Five genes including pox, pta, frdA, ldhD, and pflB were knocked out in K. oxytoca PDL-0 to decrease the production of byproducts like acetate, succinate, lactate, and formate. Using fed-batch fermentation of K. oxytoca PDL-0 ΔpoxΔptaΔfrdAΔldhDΔpflB, 74.9 g/L 2,3-BD was produced with a productivity of 2.27 g/L/h and a yield of 0.43 g/g from lactose. In addition, when whey powder was used as the substrate, 65.5 g/L 2,3-BD was produced within 24 h with a productivity of 2.73 g/L/h and a yield of 0.44 g/g.Conclusion: This study proved the efficiency of K. oxytoca PDL-0 to metabolize whey for 2,3-BD production. Due to its characteristics of non-pathogenicity and efficient lactose utilization, K. oxytoca PDL-0 might also be used in the production of other important chemicals using whey as the substrate.

scholarly journals A novel model-based control strategy for aerobic filamentous fungal fed-batch fermentation processes

2017 ◽  
Vol 114 (7) ◽  
pp. 1459-1468 ◽  
Author(s):  
Lisa Mears ◽  
Stuart M. Stocks ◽  
Mads O. Albaek ◽  
Benny Cassells ◽  
Gürkan Sin ◽  
...  
Keyword(s):  
Control Strategy ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Model Based Control ◽  
Fermentation Processes ◽  
Model Based ◽  
Fed Batch Fermentation ◽  
Novel Model

scholarly journals Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

2020 ◽  
Author(s):  
Wensi Meng ◽  
Yongjia Zhang ◽  
Menghao Cao ◽  
Wen Zhang ◽  
Chuanjuan Lü ◽  
...  
Keyword(s):  
Environmental Pollution ◽  
Batch Fermentation ◽  
Recombinant Strain ◽  
Klebsiella Oxytoca ◽  
Fermentable Sugar ◽  
Fed Batch ◽  
Whey Powder ◽  
Fed Batch Fermentation ◽  
Lactose Utilization ◽  
By Products

Abstract Background: Whey is a major pollutant generated by the dairy industry. To decrease environmental pollution caused by the industrial release of whey, new prospects for its utilization need to be urgently explored. Here, we investigated the possibility of using whey powder to produce 2,3-butanediol (BDO), an important platform chemical. Results: Klebsiella oxytoca strain PDL-0 was selected because of its ability to efficiently produce BDO from lactose, the major fermentable sugar in whey. After deleting genes pox , pta , frdA , ldhD , and pflB responding for the production of by-products acetate, succinate, lactate, and formate, a recombinant strain K. oxytoca PDL-K5 was constructed. Fed-batch fermentation using K. oxytoca PDL-K5 produced 74.9 g/L BDO with a productivity of 2.27 g/L/h and a yield of 0.43 g/g from lactose. In addition, when whey powder was used as the substrate, 65.5 g/L BDO was produced within 24 h with a productivity of 2.73 g/L/h and a yield of 0.44 g/g. Conclusion: This study demonstrated the efficiency of K. oxytoca PDL-0 for BDO production from whey. Due to its non-pathogenicity and efficient lactose utilization, K. oxytoca PDL-0 might also be used in the production of other important chemicals using whey as the substrate.

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