Optimization strategies for the analysis and purification of drug discovery compounds by reversed-phase high-performance liquid chromatography with high-pH mobile phases

2004 ◽  
Vol 1030 (1-2) ◽  
pp. 43-51 ◽  
Author(s):  
Alfonso Espada ◽  
Aránzazu Marı&#x;n ◽  
Cristina Anta
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Drug Discovery ◽  
High Performance ◽  
Reversed Phase ◽  
High Ph ◽  
Mobile Phases

scholarly journals Separation and lipophilicity of some new steroid derivatives in normal- and reversed-phase high performance liquid chromatography

2011 ◽  
Vol 17 (4) ◽  
pp. 535-542 ◽  
Author(s):  
Marijana Acanski ◽  
Djura Vujuc ◽  
Suzana Jovanovic-Santa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Log P ◽  
Reversed Phase Hplc ◽  
Mobile Phases

The separation ability and retention of normal and reversed phase HPLC with, respectively, three non-polar and two polar mobile phases, have been studied by measuring the retention constants of a series of newly synthesized estrone derivatives. The separation ability and retention are discussed in terms of the nature of the solute, eluent and stationary phase. Good correlation was found between the retention constants log k0 of newly synthesized estrone derivatives obtained on C-18 column and log P calculated by different methods.

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