Combined effects of potassium chloride and ethanol as mobile phase modulators on hydrophobic interaction and reversed-phase chromatography of three insulin variants

2015 ◽  
Vol 1381 ◽  
pp. 64-73 ◽  
Author(s):  
Karolina Johansson ◽  
Søren S. Frederiksen ◽  
Marcus Degerman ◽  
Martin P. Breil ◽  
Jørgen M. Mollerup ◽  
...  
Keyword(s):  
Potassium Chloride ◽  
Hydrophobic Interaction ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Combined Effects ◽  
Reversed Phase Chromatography ◽  
Phase Modulators

Mobile phase effects in reversed-phase chromatography

1979 ◽  
Vol 185 ◽  
pp. 129-152 ◽  
Author(s):  
Wayne R. Melander ◽  
Avi Nahum ◽  
Csaba Horváth
Keyword(s):  
Mobile Phase ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Mobile Phase Effects

scholarly journals Chromatographic Methods for the Determination of Primula Acid 1 Content in Primulae extractum fluidum

2020 ◽  
Keyword(s):  
Calibration Curve ◽  
Mobile Phase ◽  
Active Component ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Chromatography ◽  
Triterpenoid Saponins ◽  
Simple Preparation ◽  
Primula Veris

Primula veris L. (Primulaceae)is healing plant, whose root is officially used to treat cough associated with cold. Other reported indications are respiratory, thoracic and mediastinal disorders. These effects are result of high contents of triterpenoid saponins and phenolic glycosides. Primula acid 1 (PA 1, also primulasaponin 1) is main active component in Primula elatior L. This paper presents an optimized high pressure liquid chromatography (HPLC) method for the determination of primula acid 1 content in Primulae extractum fluidum. TLC was performed to check for the presence of the substance of interest. The determination was performed by reversed phase chromatography using C18 as a stationary phase. The mobile phase used for separation consisted of 0.2% H3PO4 and acetonitrile. This method was validated through different parameters. The detection limit for primula acid 1 was LD=10.41 µg/ml, and the quantification limitwas LQ=34.69 µg/ml. In order to determine the content of primula acid 1, a calibration curve was constructed, and the content of primula acid 1 was calculated by the equation of the calibration curve and was 0.2793 mg per gram of extract. The results and simple preparation of sample show that HPLC is the method of choice for this type of analysis.

Mobile phase effects in reversed-phase chromatography

1979 ◽  
Vol 185 ◽  
pp. 99-109 ◽  
Author(s):  
Wayne R. Melander ◽  
Bor-Kuan Chen ◽  
Csaba Horvàth
Keyword(s):  
Mobile Phase ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Mobile Phase Effects

scholarly journals MONOLITHIC DISK FOR THE FAST CHROMATOGRAPHIC SEPARATION

2010 ◽  
Vol 5 (2) ◽  
pp. 108-114
Author(s):  
Nurul Hidayat Aprilita ◽  
Rania Bakry ◽  
Christian W Huck ◽  
Guenther K Bonn
Keyword(s):  
Free Radical ◽  
Mobile Phase ◽  
Radical Copolymerization ◽  
Reversed Phase ◽  
Rapid Separation ◽  
Free Radical Copolymerization ◽  
Reversed Phase Chromatography ◽  
Styrene Divinylbenzene ◽  
Separation Of Proteins

Poly(styrene/divinylbenzene) (PS/DVB) monolithic disk was prepared by in situ free-radical copolymerization of styrene and divinylbenzene in the presence of decanol and tetrahydrofuran as porogens. PS/DVB monolithic disks were produced in two different lengths 1.5 mm and 3 mm. The disks were used in reversed phase chromatography of proteins with 0.2 % trifuoroacetic acid (TFA) and 0.2 % TFA in acetonitrile as mobile phase A and B, respectively. The effect of gradient rate, flow rate, temperature and disk length on the separation of proteins were also studied. PS/DVB monolithic disks allow the rapid separation of proteins in reversed phase chromatography. Keywords: monolithic disk, poly(styrene/divinylbenzene), proteins

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