A Retention Model for Polar Selectivity in Reversed Phase Chromatography as a Function of Mobile Phase Organic Modifier Type

2005 ◽  
Vol 28 (9) ◽  
pp. 1345-1356 ◽  
Author(s):  
Ping Zhuang ◽  
Richard A. Thompson ◽  
Thomas P. O'Brien
Keyword(s):  
Mobile Phase ◽  
Reversed Phase ◽  
Organic Modifier ◽  
Retention Model ◽  
Reversed Phase Chromatography

Mobile phase effects in reversed-phase chromatography

1979 ◽  
Vol 185 ◽  
pp. 129-152 ◽  
Author(s):  
Wayne R. Melander ◽  
Avi Nahum ◽  
Csaba Horváth
Keyword(s):  
Mobile Phase ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Mobile Phase Effects

scholarly journals Preparation and Hydro-Lipophilic Properties of Methoxylated and Methylated 1-Hydroxynaphthalene-2-Carboxanilides

Proceedings ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 43 ◽  
Author(s):  
Iva Kapustikova ◽  
Tomas Gonec ◽  
Jiri Kos ◽  
Ewelina Spaczynska ◽  
Michal Oravec ◽  
...  
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Chemical Structure ◽  
Reversed Phase ◽  
Log P ◽  
Organic Modifier ◽  
Chromatography Method ◽  
P Values ◽  
Liquid Chromatography Method ◽  
Substituted 1

A series of variously methoxylated and methylated N-aryl-1-hydroxynaphthalene-2-carboxanilides was prepared and characterized as potential anti-invasive agents. As it is known that lipophilicity significantly influences the biological activity of compounds, the hydro-lipophilic properties of these mono-, di- and tri-substituted 1-hydroxynaphthalene-2-carboxanilides are investigated in the study. All the discussed hydroxynaphthalene derivatives were analyzed using the reversed-phase, high-performance liquid chromatography method, to measure lipophilicity. The procedure was performed under isocratic conditions with methanol as an organic modifier in the mobile phase, using an end-capped, non-polar C18 stationary reversed-phase column. The present study discusses the correlations between the logarithm of the capacity factor k and log P/Clog P values, calculated in various ways, as well as the relationships between the lipophilicity and the chemical structure of the studied compounds.

scholarly journals Chromatographic Methods for the Determination of Primula Acid 1 Content in Primulae extractum fluidum

2020 ◽  
Keyword(s):  
Calibration Curve ◽  
Mobile Phase ◽  
Active Component ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Chromatography ◽  
Triterpenoid Saponins ◽  
Simple Preparation ◽  
Primula Veris

Primula veris L. (Primulaceae)is healing plant, whose root is officially used to treat cough associated with cold. Other reported indications are respiratory, thoracic and mediastinal disorders. These effects are result of high contents of triterpenoid saponins and phenolic glycosides. Primula acid 1 (PA 1, also primulasaponin 1) is main active component in Primula elatior L. This paper presents an optimized high pressure liquid chromatography (HPLC) method for the determination of primula acid 1 content in Primulae extractum fluidum. TLC was performed to check for the presence of the substance of interest. The determination was performed by reversed phase chromatography using C18 as a stationary phase. The mobile phase used for separation consisted of 0.2% H3PO4 and acetonitrile. This method was validated through different parameters. The detection limit for primula acid 1 was LD=10.41 µg/ml, and the quantification limitwas LQ=34.69 µg/ml. In order to determine the content of primula acid 1, a calibration curve was constructed, and the content of primula acid 1 was calculated by the equation of the calibration curve and was 0.2793 mg per gram of extract. The results and simple preparation of sample show that HPLC is the method of choice for this type of analysis.

Separation of metal-cyanide complexes by reversed-phase ion-interaction high-performance liquid chromatography

1994 ◽  
Vol 72 (2) ◽  
pp. 269-273 ◽  
Author(s):  
Louis Giroux ◽  
Dwight J. Barkley
Keyword(s):  
Ionic Strength ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Organic Modifier ◽  
Experimental Conditions ◽  
Metal Cyanide ◽  
Cyanide Complexes ◽  
Cyano Complexes ◽  
Phase Differences

Reversed-phase ion-interaction chromatography has been used to study the separation of Cu(I), Ag(I), Ni(II), Au(I), Co(III), Fe(III), and Fe(II) cyano complexes on silica and carbon-based reversed-phases with UV detection at 215 nm. Separation of the metal-cyanide complexes was affected by a number of experimental factors including the nature and concentration of the organic modifier, nature and concentration of the ion-pairing reagent, pH, and ionic strength of the mobile phase. Differences between the elution order of metallo-cyanides observed in the present work and in other investigations under similar experimental conditions are explained by a difference in the ionic strength of the mobile phase. Finally, this technique is used to analyze metal-cyanide complexes in a gold mill solution.

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