Particle enhanced turbidimetric immunoassay for the determination of urine cystatin C on Cobas c501

Abstract Background: Serum cystatin C, a cysteine protease inhibitor, has been suggested as a new marker of glomerular filtration rate (GFR). This study explored the possibility of replacing the creatinine clearance (CrCl) estimation of GFR with cystatin C in early detection of renal impairment in cancer patients on chemotherapy. Methods: Serum creatinine and cystatin C concentrations as well as 24-h CrCl were determined simultaneously in 72 cancer patients. Among them, 60 were treated with combined chemotherapy with cisplatin (CDDP). Creatinine was determined enzymatically with a spectrophotometric method. Serum cystatin C was determined by a particle-enhanced turbidimetric immunoassay. Results: Cystatin C and creatinine correlated significantly (P = 0.001) with CrCl. The correlation was significantly better for cystatin C than creatinine (r = 0.84 vs 0.74; P = 0.01). Stepwise regression analysis identified no differences for the correlations between cystatin C and CrCl in patients with or without metastases (r = 0.82 and 0.84, respectively) as well as before treatment and before the fourth cycle of chemotherapy (r = 0.70 and 0.75, respectively). A cystatin C cutoff concentration of 1.33 mg/L had 87% sensitivity and 100% specificity for detecting CrCl <78 mL/min. ROC analysis indicated that cystatin C was superior to serum creatinine for predicting CrCl <78 mL/min (P <0.04). Conclusions: Serum cystatin C is superior to serum creatinine for detection of decreased CrCl and potentially for the estimation of GFR in cancer patients independent of the presence of metastases or chemotherapy.

Download Full-text

Measurement of cystatin C in human urine by particle-enhanced turbidimetric immunoassay on an automated biochemistry analyzer

Clinical Biochemistry ◽

10.1016/j.clinbiochem.2013.05.072 ◽

2013 ◽

Vol 46 (12) ◽

pp. 1128-1130 ◽

Cited By ~ 7

Author(s):

Konstantinos Makris ◽

Efthimia Nikolaki ◽

Konstantinos Nanopoulos ◽

Konstantinos M. Pirgakis ◽

Chrisostomos K. Maltezos

Keyword(s):

Cystatin C ◽

Human Urine ◽

Turbidimetric Immunoassay

Download Full-text

Determination of the production rate and non‐renal clearance of cystatin C and estimation of the glomerular filtration rate from the serum concentration of cystatin C in humans

Scandinavian Journal of Clinical and Laboratory Investigation ◽

10.1080/00365510510013523 ◽

2005 ◽

Vol 65 (2) ◽

pp. 111-124 ◽

Cited By ~ 114

Author(s):

P. Sjöström ◽

M. Tidman ◽

I. Jones

Keyword(s):

Glomerular Filtration Rate ◽

Serum Concentration ◽

Glomerular Filtration ◽

Renal Clearance ◽

Production Rate ◽

Cystatin C ◽

Filtration Rate

Download Full-text

Determination of cerebrospinal fluid cystatin C on Architect ci8200

Journal of Immunological Methods ◽

10.1016/j.jim.2010.06.012 ◽

2010 ◽

Vol 360 (1-2) ◽

pp. 84-88 ◽

Cited By ~ 3

Author(s):

Annaby Moussa Ndjole ◽

Constantin Bodolea ◽

Tom Nilsen ◽

Torsten Gordh ◽

Mats Flodin ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Cystatin C

Download Full-text

Comparison of Serum Creatinine and Cystatin C for Early Diagnosis of Contrast-Induced Nephropathy after Coronary Angiography and Interventions

Clinical Chemistry ◽

10.1373/clinchem.2011.170464 ◽

2012 ◽

Vol 58 (2) ◽

pp. 458-464 ◽

Cited By ~ 29

Author(s):

Flavio Ribichini ◽

Giovanni Gambaro ◽

Maria Stella Graziani ◽

Michele Pighi ◽

Gabriele Pesarini ◽

...

Keyword(s):

Serum Creatinine ◽

Diagnostic Accuracy ◽

Cystatin C ◽

Roc Curve ◽

Diagnostic Value ◽

Roc Curve Analysis ◽

Contrast Induced Nephropathy ◽

Early Predictors ◽

Patients At Risk

Abstract BACKGROUND The diagnostic accuracy of serum creatinine and cystatin C (Cys) as early predictors of contrast-induced nephropathy (CIN) has been debated. We investigated the diagnostic sensitivities, diagnostic specificities, and variations from baseline for serum creatinine and Cys in CIN. METHODS We prospectively evaluated 166 patients at risk for CIN at baseline, and at 12, 24, and 48 h after exposure to contrast media. CIN occurred in 30 patients (18%). Changes (Δ) compared to baseline in serum creatinine and Cys were evaluated at the predefined time points. ROC curve analysis was performed for the Δ 12-h basal serum creatinine and Cys. RESULTS The Δ serum creatinine at 12 h from baseline was the earliest predictor of CIN [area under the ROC curve (AUC) = 0.80; P < 0.001]. The Δ serum creatinine 15% variation [0.15 mg/dL (13.2 μmol/L)] yielded 43% diagnostic sensitivity and 93% diagnostic specificity. The ΔCys at 12 h from baseline performed significantly worse than serum creatinine (AUC = 0.48; P = 0.74). CONCLUSIONS Variations from the serum creatinine baseline offer better diagnostic accuracy for predicting CIN at an earlier stage than similar variations in Cys. An additional diagnostic value of Cys over the determination of serum creatinine in the setting of CIN was not observed.

Download Full-text

Preliminary evaluation of the performance of a new, highly sensitive commercial immunoassay for serum ferritin determination

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2005.149 ◽

2005 ◽

Vol 43 (8) ◽

Cited By ~ 5

Author(s):

Paolo Signò ◽

Alessandra Barassi ◽

Raffaele Novario ◽

Gian Vico Melzi d'Eril

Keyword(s):

Serum Ferritin ◽

Analytical Performance ◽

Analysis Time ◽

Preliminary Evaluation ◽

Highly Sensitive ◽

Turbidimetric Immunoassay

AbstractWe evaluated the analytical performance of a new, commercial, fully automated immunoturbidimetric assay for the determination of ferritin [FER-Latex(X2)CN SEIKEN, Denka Seiken, Japan] in serum on the Olympus AU2700 analyzer. The new assay is a latex-enhanced turbidimetric immunoassay with an analysis time of 10min. The linearity of the assay was confirmed up to 2505pmol/L (R

Download Full-text