Measurement of cystatin C in human urine by particle-enhanced turbidimetric immunoassay on an automated biochemistry analyzer

Abstract Background: Serum cystatin C, a cysteine protease inhibitor, has been suggested as a new marker of glomerular filtration rate (GFR). This study explored the possibility of replacing the creatinine clearance (CrCl) estimation of GFR with cystatin C in early detection of renal impairment in cancer patients on chemotherapy. Methods: Serum creatinine and cystatin C concentrations as well as 24-h CrCl were determined simultaneously in 72 cancer patients. Among them, 60 were treated with combined chemotherapy with cisplatin (CDDP). Creatinine was determined enzymatically with a spectrophotometric method. Serum cystatin C was determined by a particle-enhanced turbidimetric immunoassay. Results: Cystatin C and creatinine correlated significantly (P = 0.001) with CrCl. The correlation was significantly better for cystatin C than creatinine (r = 0.84 vs 0.74; P = 0.01). Stepwise regression analysis identified no differences for the correlations between cystatin C and CrCl in patients with or without metastases (r = 0.82 and 0.84, respectively) as well as before treatment and before the fourth cycle of chemotherapy (r = 0.70 and 0.75, respectively). A cystatin C cutoff concentration of 1.33 mg/L had 87% sensitivity and 100% specificity for detecting CrCl <78 mL/min. ROC analysis indicated that cystatin C was superior to serum creatinine for predicting CrCl <78 mL/min (P <0.04). Conclusions: Serum cystatin C is superior to serum creatinine for detection of decreased CrCl and potentially for the estimation of GFR in cancer patients independent of the presence of metastases or chemotherapy.

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Particle enhanced turbidimetric immunoassay for the determination of urine cystatin C on Cobas c501

Clinical Biochemistry ◽

10.1016/j.clinbiochem.2011.12.027 ◽

2012 ◽

Vol 45 (4-5) ◽

pp. 339-344 ◽

Cited By ~ 10

Author(s):

Azita Sohrabian ◽

Feria Hikmet Noraddin ◽

Mats Flodin ◽

Annika Fredricsson ◽

Anders Larsson

Keyword(s):

Cystatin C ◽

Turbidimetric Immunoassay

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Measurement of Urinary Cystatin C with a Particle-Enhanced Turbidimetric Immunoassay on Architect Ci8200

Journal of Clinical Laboratory Analysis ◽

10.1002/jcla.21531 ◽

2012 ◽

Vol 26 (5) ◽

pp. 358-364 ◽

Cited By ~ 5

Author(s):

Feria Hikmet Noraddin ◽

Mats Flodin ◽

Annika Fredricsson ◽

Azita Sohrabian ◽

Anders Larsson

Keyword(s):

Cystatin C ◽

Urinary Cystatin C ◽

Turbidimetric Immunoassay

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Cystatin C analysis in the dog: A comparison of turbidimetric and nephelometric assay results

Acta Veterinaria Hungarica ◽

10.1556/avet.58.2010.1.6 ◽

2010 ◽

Vol 58 (1) ◽

pp. 59-67 ◽

Cited By ~ 3

Author(s):

Paweł Jonkisz ◽

Krisztina Kungl ◽

Agnieszka Sikorska ◽

Agnieszka Kurosad ◽

Józef Nicpoń

Keyword(s):

Renal Function ◽

Correlation Coefficient ◽

Cystatin C ◽

Serum Urea ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Human Cystatin C ◽

Lower Correlation ◽

Human Cystatin ◽

Turbidimetric Immunoassay

Cystatin C is a serum protein with low molecular mass, which has been suggested as a marker to assess renal function in the dog. This protein is regularly assessed using particle-enhanced turbidimetric immunoassay (PETIA) and particle-enhanced nephelometric immunoassay (PENIA), in which rabbit anti-human cystatin C antibodies are used. The purpose of this work was to compare the results of cystatin C analysis obtained by PETIA and PENIA assays in the dog. Forty dogs of different genders and breeds were classified into four groups of 10 animals each based on serum creatinine concentrations (4 stages of chronic kidney disease). Serum cystatin C concentration was measured using PETIA and PENIA assays, the results were compared, and correlation with serum urea and creatinine concentrations was established. The correlation coefficient for results obtained using PETIA and PENIA assays was r = 0.706. Serum cystatin C concentrations obtained in PETIA had a lower correlation coefficient with creatinine concentrations than those found in PENIA (r = 0.614 and r = 0.904, respectively); similarly, serum cystatin C was less correlated with serum urea concentration in PETIA than in PENIA (r = 0.463 and r = 0.636, respectively). The results obtained in this study suggest that the nephelometric assay is more sensitive and was shown to be more closely correlated with other renal function indicators than the PETIA assay.

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Determination of Cystatin C in human urine by isotope dilution tandem mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2019.112889 ◽

2020 ◽

Vol 177 ◽

pp. 112889

Author(s):

Amanda Suárez-Fernández ◽

Ana González-Antuña ◽

Pablo Rodríguez-González ◽

J. Ignacio García Alonso

Keyword(s):

Mass Spectrometry ◽

Tandem Mass Spectrometry ◽

Cystatin C ◽

Isotope Dilution ◽

Human Urine ◽

Tandem Mass

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Novel liquid chromatographic determination of cystatin C in human urine

Journal of Chromatography B ◽

10.1016/j.jchromb.2009.02.009 ◽

2009 ◽

Vol 877 (8-9) ◽

pp. 747-750 ◽

Cited By ~ 4

Author(s):

Othman Ibrahem Yousef Al-Musaimi ◽

Manar Khalid Fayyad ◽

Adel Khalil Mishal

Keyword(s):

Cystatin C ◽

Human Urine ◽

Chromatographic Determination ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

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Initial evaluation of cystatin C measurement by particle-enhanced immunonephelometry on the Behring nephelometer systems (BNA, BN II)

Clinical Chemistry ◽

10.1093/clinchem/43.6.1016 ◽

1997 ◽

Vol 43 (6) ◽

pp. 1016-1022 ◽

Cited By ~ 191

Author(s):

Hazel Finney ◽

David J Newman ◽

Walter Gruber ◽

Peter Merle ◽

Christopher P Price

Keyword(s):

Glomerular Filtration Rate ◽

Rheumatoid Factor ◽

Cystatin C ◽

Filtration Rate ◽

Mean Difference ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Upper Limit ◽

Analytical Recovery ◽

Turbidimetric Immunoassay

Abstract Serum cystatin C has been suggested as a new marker of glomerular filtration rate (GFR). We describe a fully automated and rapid particle-enhanced nephelometric immunoassay (PENIA) for measuring serum cystatin C on the Behring nephelometer systems (BNA, BN II). Each sample is analyzed in 6 min with as many as 75 samples per batch. The assay covers the range 0.23–7.25 mg/L, up to seven times the upper limit of normal. The intra- and interassay imprecision are <3.3% and <4.5%, respectively. There is absolute linearity across the assay range (r2 = 0.997), with analytical recovery by cystatin C addition between 95% and 109% (mean 102%). Hemoglobin (≤8.0 g/L), bilirubin (≤488 μL), triglycerides (≤23 mmol/L), rheumatoid factor (≤2000 kIU/L), and myeloma paraprotein (≤41 g/L) do not interfere with the assay. This assay agreed well with an in-house particle-enhanced turbidimetric immunoassay (PETIA) (mean difference = 1.73 ± 2.10) and a commercial PETIA (mean difference = 1.13 ± 0.86). This is a new assay by which cystatin C may be effectively used as a marker of GFR estimation.

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