Sandwich-likely structured, magnetically-driven recovery, biomimetic composite penicillin G acylase-based biocatalyst with excellent operation stability

2022 ◽  
pp. 128245
Author(s):  
Chunli Liu ◽  
Yongshan Zhou ◽  
Gang Wu ◽  
Kaikai Gao ◽  
Lin Li ◽  
...  
Keyword(s):  
Penicillin G Acylase ◽  
Penicillin G ◽  
Operation Stability

Studies on Immobilization of Penicillin G Acylase to Epoxy Resin

2012 ◽  
Vol 512-515 ◽  
pp. 1699-1711 ◽  
Author(s):  
Chao Fan ◽  
Ji Lie Li ◽  
Xiao Yuan Zhu ◽  
Nan Li ◽  
Wei Wang ◽  
...  
Keyword(s):  
Epoxy Resin ◽  
Immobilized Enzyme ◽  
Continuous Operation ◽  
Penicillin G Acylase ◽  
Penicillin G ◽  
Process Conditions ◽  
Optimal Conditions ◽  
Optimum Temperature ◽  
Optimum Ph ◽  
Operation Stability

The process conditions of immobilizing penicillin G acylase(PGA) by epoxy resin were studied. This experiment used the Box-Behnken experimental design and response surface methodology(RSM) to optimize the conditions of immobilizing PGA by epoxy resin. The results showed the best process conditions were pH 8.1, temperature 29°C, carrier of epoxy resin 1g and reaction time 24 h. On these conditions, the activity of the immobilized enzyme was 365.76 U g-1, activity recovery rate was 62.82%. The characteristics of the immobilized PGA under optimal conditions had been measured and found that the optimum pH of immobilized enzyme was 9.0, the optimum temperature was 60°C. It has better continuous operation stability.

Resolution of α/β-amino acids by enantioselective penicillin G acylase from Achromobacter sp .

2015 ◽  
Vol 122 ◽  
pp. 240-247 ◽  
Author(s):  
Michal Grulich ◽  
Jan Brezovský ◽  
Václav ŠtĿpánek ◽  
Andrea Palyzová ◽  
Eva Kyslíková ◽  
...  
Keyword(s):  
Amino Acids ◽  
Penicillin G Acylase ◽  
Penicillin G ◽  
Achromobacter Sp

Porous thiiranyl polymers: Newer supports for immobilization of penicillin G acylase

1997 ◽  
Vol 33 (9) ◽  
pp. 1481-1485 ◽  
Author(s):  
S. Skaria ◽  
E.Sreenivasa Rao ◽  
S. Ponrathnam ◽  
K.K. Kumar ◽  
J.G. Shewale
Keyword(s):  
Penicillin G Acylase ◽  
Penicillin G

Inoculum Studies in Production of Penicillin G Acylase by Bacillus megaterium ATCC 14945

2002 ◽  
pp. 679-686 ◽  
Author(s):  
Laura M. Pinotti ◽  
Rosineide G. Silva ◽  
Roberto C. Giordano ◽  
Raquel L. C. Giordano
Keyword(s):  
Bacillus Megaterium ◽  
Penicillin G Acylase ◽  
Penicillin G

scholarly journals <italic>In vivo</italic> imaging of penicillin G acylase in mice via a near-infrared fluorescent probe

2017 ◽  
Vol 47 (8) ◽  
pp. 994-999
Author(s):  
Xiaohua Li ◽  
Xinyuan He ◽  
Wen Shi ◽  
Haolin Zhang ◽  
Huimin Ma
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Penicillin G Acylase ◽  
Penicillin G
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