A new method to obtain the repassivation time of passive materials based on the single particle impingement

2020 ◽  
Vol 170 ◽  
pp. 108717
Author(s):  
Zhengbin Wang ◽  
Chong Sun ◽  
Linlin Li ◽  
Morteza Roostaei ◽  
Vahidoddin Fattahpour ◽  
...  
Keyword(s):  
Single Particle ◽  
New Method ◽  
Passive Materials

scholarly journals Detecting Transient Trapping from a Single Trajectory: A Structural Approach

Entropy ◽  
2021 ◽  
Vol 23 (8) ◽  
pp. 1044
Author(s):  
Yann Lanoiselée ◽  
Jak Grimes ◽  
Zsombor Koszegi ◽  
Davide Calebiro
Keyword(s):  
Plasma Membrane ◽  
Single Particle ◽  
Adrenergic Receptors ◽  
Particle Trajectory ◽  
Temporal Structure ◽  
Structural Approach ◽  
New Method ◽  
Trajectory Data ◽  
Receptor Stimulation ◽  
Over Time

In this article, we introduce a new method to detect transient trapping events within a single particle trajectory, thus allowing the explicit accounting of changes in the particle’s dynamics over time. Our method is based on new measures of a smoothed recurrence matrix. The newly introduced set of measures takes into account both the spatial and temporal structure of the trajectory. Therefore, it is adapted to study short-lived trapping domains that are not visited by multiple trajectories. Contrary to most existing methods, it does not rely on using a window, sliding along the trajectory, but rather investigates the trajectory as a whole. This method provides useful information to study intracellular and plasma membrane compartmentalisation. Additionally, this method is applied to single particle trajectory data of β2-adrenergic receptors, revealing that receptor stimulation results in increased trapping of receptors in defined domains, without changing the diffusion of free receptors.

scholarly journals A quantitative nanoparticle extraction method for microsecond time resolved single-particle ICP-MS data in the presence of a high background

2019 ◽  
Vol 34 (8) ◽  
pp. 1571-1580 ◽  
Author(s):  
Darya Mozhayeva ◽  
Carsten Engelhard
Keyword(s):  
Time Resolution ◽  
Single Particle ◽  
Extraction Method ◽  
New Method ◽  
Time Resolved ◽  
High Background ◽  
Icp Ms ◽  
Dissolved Ions

A new method for the quantification of dissolved ions and nanoparticles in mixtures with SP-ICP-MS with microsecond time resolution.

scholarly journals Toward Real-Time Monitoring and Control of Single Nanoparticle Properties with a Microbubble Resonator Spectrometer

2019 ◽  
Author(s):  
Levi Hogan ◽  
Erik Horak ◽  
Jonathan Ward ◽  
Kassandra Knapper ◽  
Síle Nic Chormaic ◽  
...  
Keyword(s):  
Single Particle ◽  
Stable Solution ◽  
New Method ◽  
Monitoring And Control ◽  
Experimental Conditions ◽  
Widespread Application ◽  
Time Resolved ◽  
Biologically Relevant ◽  
Rapid Acquisition ◽  
Free Environment

<p></p><p></p><p>Optical microresonators have widespread application at the frontiers of nanophotonic technology, driven by their ability to confine light to the nanoscale and enhance light-matter interactions. Microresonators form the heart of a new method for single-particle photothermal absorption spectroscopy, whereby the microresonators act as microscale thermometers to detect the heat dissipated by optically pumped, non-luminescent nanoscopic targets. However, translation of this technology to chemically dynamic systems requires a platform that is mechanically stable, solution compatible, and visibly transparent. We report microbubble absorption spectrometers as a new and versatile platform that meets these requirements. Microbubbles integrate a two-port microfluidic device within a Whispering Gallery Mode (WGM) microresonator, allowing for the facile exchange of chemical reagents within the resonator’s interior while maintaining a solution-free environment on its exterior. We first leverage these qualities to investigate the photo-activated etching of single gold nanorods by ferric chloride, providing a new method for rapid acquisition of spatial and morphological information about nanoparticles as they undergo chemical reactions. We then demonstrate the ability to control nanorod orientation within a microbubble through optically exerted torque, a new route toward the construction of hybrid photonic-plasmonic systems. Critically, the reported platform advances microresonator spectrometer technology by permitting room-temperature, aqueous experimental conditions, opening a regime of time-resolved single-particle experiments on non-emissive, nanoscale analytes engaged in catalytically and biologically relevant chemical dynamics.</p><p></p><p></p>

scholarly journals A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis

IUCrJ ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 5-17 ◽  
Author(s):  
Jasenko Zivanov ◽  
Takanori Nakane ◽  
Sjors H. W. Scheres
Keyword(s):  
Single Particle ◽  
Gaussian Process Regression ◽  
New Method ◽  
Particle Analysis ◽  
Data Sets ◽  
Single Particle Analysis ◽  
A Posteriori ◽  
Particle Trajectories ◽  
Hard Constraints ◽  
Induced Motion

A new method to estimate the trajectories of particle motion and the amount of cumulative beam damage in electron cryo-microscopy (cryo-EM) single-particle analysis is presented. The motion within the sample is modelled through the use of Gaussian process regression. This allows a prior likelihood that favours spatially and temporally smooth motion to be associated with each hypothetical set of particle trajectories without imposing hard constraints. This formulation enables the a posteriori likelihood of a set of particle trajectories to be expressed as a product of that prior likelihood and an observation likelihood given by the data, and this a posteriori likelihood to then be maximized. Since the smoothness prior requires three parameters that describe the statistics of the observed motion, an efficient stochastic method to estimate these parameters is also proposed. Finally, a practical algorithm is proposed that estimates the average amount of cumulative radiation damage as a function of radiation dose and spatial frequency, and then fits relative B factors to that damage in a robust way. The method is evaluated on three publicly available data sets, and its usefulness is illustrated by comparison with state-of-the-art methods and previously published results. The new method has been implemented as Bayesian polishing in RELION-3, where it replaces the existing particle-polishing method, as it outperforms the latter in all tests conducted.

Toward Real-Time Monitoring and Control of Single Nanoparticle Properties with a Microbubble Resonator Spectrometer

2019 ◽  
Author(s):  
Levi Hogan ◽  
Erik Horak ◽  
Jonathan Ward ◽  
Kassandra Knapper ◽  
Síle Nic Chormaic ◽  
...  
Keyword(s):  
Single Particle ◽  
Stable Solution ◽  
New Method ◽  
Monitoring And Control ◽  
Experimental Conditions ◽  
Widespread Application ◽  
Time Resolved ◽  
Biologically Relevant ◽  
Rapid Acquisition ◽  
Free Environment

<p></p><p></p><p>Optical microresonators have widespread application at the frontiers of nanophotonic technology, driven by their ability to confine light to the nanoscale and enhance light-matter interactions. Microresonators form the heart of a new method for single-particle photothermal absorption spectroscopy, whereby the microresonators act as microscale thermometers to detect the heat dissipated by optically pumped, non-luminescent nanoscopic targets. However, translation of this technology to chemically dynamic systems requires a platform that is mechanically stable, solution compatible, and visibly transparent. We report microbubble absorption spectrometers as a new and versatile platform that meets these requirements. Microbubbles integrate a two-port microfluidic device within a Whispering Gallery Mode (WGM) microresonator, allowing for the facile exchange of chemical reagents within the resonator’s interior while maintaining a solution-free environment on its exterior. We first leverage these qualities to investigate the photo-activated etching of single gold nanorods by ferric chloride, providing a new method for rapid acquisition of spatial and morphological information about nanoparticles as they undergo chemical reactions. We then demonstrate the ability to control nanorod orientation within a microbubble through optically exerted torque, a new route toward the construction of hybrid photonic-plasmonic systems. Critically, the reported platform advances microresonator spectrometer technology by permitting room-temperature, aqueous experimental conditions, opening a regime of time-resolved single-particle experiments on non-emissive, nanoscale analytes engaged in catalytically and biologically relevant chemical dynamics.</p><p></p><p></p>

scholarly journals A Bayesian Approach to Beam-Induced Motion Correction in Cryo-EM Single-Particle Analysis

2018 ◽  
Author(s):  
Jasenko Zivanov ◽  
Takanori Nakane ◽  
Sjors H. W. Scheres
Keyword(s):  
Single Particle ◽  
Gaussian Process Regression ◽  
New Method ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
A Posteriori ◽  
Particle Trajectories ◽  
Hard Constraints ◽  
Smooth Motion ◽  
Induced Motion

AbstractWe present a new method to estimate the trajectories of particle motion and the amount of cumulative beam damage in electron cryo-microscopy (cryo-EM) single particle analysis. We model the motion within the sample through the use of Gaussian Process regression. This allows us to associate with each hypothetical set of particle trajectories a prior likelihood that favours spatially and temporally smooth motion without imposing hard constraints. This formulation enables us to express the a-posteriori likelihood of a set of particle trajectories as a product of that prior likelihood and an observation likelihood given by the data, and to then maximise this a-posteriori likelihood. Since our smoothness prior requires three parameters that describe the statistics of the observed motion, we also propose an efficient stochastic method to estimate those parameters. Finally, we propose a practical means of estimating the average amount of cumulative radiation damage as a function of radiation dose and spatial frequency, and a robust method of fitting relative B-factors to it. We evaluate our method on three publicly available datasets, and illustrate its usefulness by comparison with state-of-the-art methods and previously published results. The new method has been implemented as Bayesian polishing in RELION-3, where it replaces the existing particle polishing method, as it outperforms the latter in all tests conducted.

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