A novel method for measurement of diffusion coefficients of proteins and DNA in solution

2004 ◽  
Vol 393 (4-6) ◽  
pp. 539-545 ◽  
Author(s):  
Naoki Baden ◽  
Masahide Terazima
2010 ◽  
Vol 12 (28) ◽  
pp. 8092 ◽  
Author(s):  
Olexandra Zybaylo ◽  
Osama Shekhah ◽  
Hui Wang ◽  
Maxim Tafipolsky ◽  
Rochus Schmid ◽  
...  

2015 ◽  
Vol 9 (5) ◽  
pp. 534-540 ◽  
Author(s):  
Terutake Hayashi ◽  
◽  
Yuki Ishizaki ◽  
Masaki Michihata ◽  
Yasuhiro Takaya ◽  
...  

Fluorescent polarization methods are used to detect complementary base pairing of DNA in biological fields. These methods work by measuring the rotational diffusion coefficient of Brownian motion of the fluorescent particles in solution. The rotational diffusion coefficient corresponds to the inverse third power of diameter according to the Debye-Stokes-Einstein equation for nanoparticles as hard spheres. We develop a novel method to measure the rotational diffusion coefficient using a fluorescent probe with a DNA spacer connected to a gold nanoparticle. We studied the physical characteristics of this probe to verify the feasibility of the proposed method. The rotational diffusion coefficients of gold nanoparticles with diameters ranging between 5–20 nm were measured using this developed system. In this manuscript we describe a novel fluorescent polarization method for nanoparticle sizing using a fluorescent DNA probe.


2017 ◽  
Vol 63 ◽  
pp. 342-348 ◽  
Author(s):  
Péter Polyák ◽  
Dóra Szemerszki ◽  
Henrik Christopher Benke ◽  
Béla Pukánszky

2002 ◽  
Vol 102 (6) ◽  
pp. 747-757 ◽  
Author(s):  
W. Kwaśniewski ◽  
K. Dzieciołowski ◽  
T.M. Adamowicz

2002 ◽  
Vol 312-314 ◽  
pp. 196-202 ◽  
Author(s):  
Toru Ujihara ◽  
Kozo Fujiwara ◽  
Gen Sazaki ◽  
Noritaka Usami ◽  
Kazuo Nakajima

Author(s):  
E.G. Bithell ◽  
W.M. Stobbs

It is well known that the microstructural consequences of the ion implantation of semiconductor heterostructures can be severe: amorphisation of the damaged region is possible, and layer intermixing can result both from the original damage process and from the enhancement of the diffusion coefficients for the constituents of the original composition profile. A very large number of variables are involved (the atomic mass of the target, the mass and energy of the implant species, the flux and the total dose, the substrate temperature etc.) so that experimental data are needed despite the existence of relatively well developed models for the implantation process. A major difficulty is that conventional techniques (e.g. electron energy loss spectroscopy) have inadequate resolution for the quantification of any changes in the composition profile of fine scale multilayers. However we have demonstrated that the measurement of 002 dark field intensities in transmission electron microscope images of GaAs / AlxGa1_xAs heterostructures can allow the measurement of the local Al / Ga ratio.


Author(s):  
M.A. Gregory ◽  
G.P. Hadley

The insertion of implanted venous access systems for children undergoing prolonged courses of chemotherapy has become a common procedure in pediatric surgical oncology. While not permanently implanted, the devices are expected to remain functional until cure of the primary disease is assured. Despite careful patient selection and standardised insertion and access techniques, some devices fail. The most commonly encountered problems are colonisation of the device with bacteria and catheter occlusion. Both of these difficulties relate to the development of a biofilm within the port and catheter. The morphology and evolution of biofilms in indwelling vascular catheters is the subject of ongoing investigation. To date, however, such investigations have been confined to the examination of fragments of biofilm scraped or sonicated from sections of catheter. This report describes a novel method for the extraction of intact biofilms from indwelling catheters.15 children with Wilm’s tumour and who had received venous implants were studied. Catheters were removed because of infection (n=6) or electively at the end of chemotherapy.


Sign in / Sign up

Export Citation Format

Share Document