Resistance to Melon necrotic spot virus in Cucumis melo L. ‘Doublon’ artificially inoculated by the fungus vector Olpidium bornovanus

2006 ◽  
Vol 25 (5) ◽  
pp. 426-431 ◽  
Author(s):  
Cristina Mallor ◽  
Marisol Luis-Arteaga ◽  
José María Álvarez ◽  
Celia Montaner ◽  
Elena Floris
Keyword(s):  
Cucumis Melo ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Olpidium Bornovanus ◽  
Cucumis Melo L

Melon necrotic spot virus. [Distribution map].

2010 ◽  
Author(s):  
Keyword(s):  
Cucumis Melo ◽  
Citrullus Lanatus ◽  
Necrotic Spot ◽  
Distribution Map ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Olpidium Bornovanus ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is given for Melon necrotic spot virus (Tombusviridae: Carmovirus). The hosts include melon (Cucumis melo), cucumber (Cucumis sativus) and watermelon (Citrullus lanatus). Information is given on the geographical distribution in Europe (France, Greece, Crete, Italy, Sardinia, Netherlands, Norway, Spain, Canary islands, mainland Spain, Sweden, UK, England and Wales), Asia (China, Jiangsu, Iran, Israel, Japan, Hokkaido, Honshu, Kyushu, Shikoku, Korea Republic, Syria and Turkey), Africa (Tunisia), North America (Canada, Ontario, Mexico, USA, California) Central America and Caribbean (Guatemala, Honduras and Panama) and South America (Uruguay). The virus is transmitted by the fungal vector Olpidium bornovanus (syn. O. radicale) (Chytridiomycota: Olpidiaceae).

A physical map covering the nsv locus that confers resistance to Melon necrotic spot virus in melon (Cucumis melo L.)

2005 ◽  
Vol 111 (5) ◽  
pp. 914-922 ◽  
Author(s):  
Mónica Morales ◽  
Gisella Orjeda ◽  
Cristina Nieto ◽  
Hans van Leeuwen ◽  
Amparo Monfort ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
Physical Map ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Cucumis Melo L

scholarly journals Molecular Characterization of a Melon necrotic spot virus Strain That Overcomes the Resistance in Melon and Nonhost Plants

2004 ◽  
Vol 17 (6) ◽  
pp. 668-675 ◽  
Author(s):  
Juan A. Díaz ◽  
Cristina Nieto ◽  
Enrique Moriones ◽  
Verónica Truniger ◽  
Miguel A. Aranda
Keyword(s):  
Cdna Clone ◽  
Recessive Gene ◽  
Genomic Region ◽  
Open Reading Frames ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Gomphrena Globosa ◽  
Resistance Trait ◽  
Cucumis Melo L

Resistance of melon (Cucumis melo L.) to Melon necrotic spot virus (MNSV) is inherited as a single recessive gene, denoted nsv. No MNSV isolates described to date (e.g., MNSV-Mα5), except for the MNSV-264 strain described here, are able to overcome the resistance conferred by nsv. Analysis of protoplasts of susceptible (Nsv/-) and resistant (nsv/nsv) melon cultivars inoculated with MNSV-264 or MNSV-Mα5 indicated that the resistance trait conferred by this gene is expressed at the single-cell level. The nucleotide sequence of the MNSV-264 genome has a high nucleotide identity with the sequences of other MNSV isolates, with the exception of its genomic 3′-untranslated region (3′-UTR), where less than 50% of the nucleotides are shared between MNSV-264 and the other two MNSV isolates completely sequenced to date. Uncapped RNAs transcribed from a full-length MNSV-264 cDNA clone were infectious and caused symptoms indistinguishable from those caused by the parental viral RNA. This cDNA clone allowed generation of chimeric mutants between MNSV-264 and MNSV-Mα5 through the exchange of the last 74 nucleotides of their coat protein (CP) open reading frames and the complete 3′-UTRs. Analysis of protoplasts of susceptible and resistant melon cultivars inoculated with chimeric mutants clearly showed that the MNSV avirulence determinant resides in the exchanged region. The carboxy-termini of the CP of both isolates are identical; therefore, the avirulence determinant likely consists of the RNA sequence itself. We also demonstrated that this genomic region contains the determinant for the unique ability of the isolate MNSV-264 to infect noncucurbit hosts (Nicotiana benthamiana and Gomphrena globosa).

scholarly journals Isolation of a novel antagonistic Bacillus sp. against Olpidium bornovanus, the fungal vector of Melon necrotic spot virus.

2008 ◽  
Vol 74 (3) ◽  
pp. 148-152 ◽  
Author(s):  
Y. IKEGASHIRA ◽  
T. OHKI ◽  
K. MATSUO ◽  
M. AINO ◽  
H. KAJIHARA ◽  
...  
Keyword(s):  
Bacillus Sp ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Olpidium Bornovanus

scholarly journals A New Strain of Melon necrotic spot virus that Is Unable to Systemically Infect Cucumis melo

2008 ◽  
Vol 98 (11) ◽  
pp. 1165-1170 ◽  
Author(s):  
Takehiro Ohki ◽  
Isamu Sako ◽  
Ayami Kanda ◽  
Tomofumi Mochizuki ◽  
Yohachiro Honda ◽  
...  
Keyword(s):  
Coat Protein ◽  
Cucumis Melo ◽  
Genomic Structure ◽  
Single Cell Level ◽  
Necrotic Spot ◽  
Cell Level ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Necrotic Spots ◽  
Necrotic Symptoms

We report a new strain of Melon necrotic spot virus (MNSV) that is unable to systemically infect Cucumis melo. A spherical virus (W-isolate), about 30 nm in diameter like a carmovirus, was isolated from watermelons with necrotic symptoms. The W-isolate had little serological similarity to MNSV, and it did not cause any symptoms in six melon cultivars susceptible to MNSV; however, the host range of the W-isolate was limited exclusively to cucurbitaceous plants, and transmission by O. bornovanus was confirmed. Its genomic structure was identical to that of MNSV, and its p89 protein and coat protein (CP) showed 81.6 to 83.2% and 74.1 to 75.1% identity to those of MNSV, respectively. Analysis of protoplast showed that the W-isolate replicated in melons at the single-cell level. Furthermore, chimeric clones carrying the CP of MNSV induced necrotic spots in melons. These results suggested that the absence of symptoms in melons was due to a lack of ability of the W-isolate to move from cell to cell. In view of these findings, we propose that the new isolate should be classified as a novel MNSV watermelon strain.

scholarly journals The protruding domain of the coat protein of Melon necrotic spot virus is involved in compatibility with and transmission by the fungal vector Olpidium bornovanus

Virology ◽  
2010 ◽  
Vol 402 (1) ◽  
pp. 129-134 ◽  
Author(s):  
Takehiro Ohki ◽  
Fusamichi Akita ◽  
Tomofumi Mochizuki ◽  
Ayami Kanda ◽  
Takahide Sasaya ◽  
...  
Keyword(s):  
Coat Protein ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Olpidium Bornovanus
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