scholarly journals Molecular Characterization of a Melon necrotic spot virus Strain That Overcomes the Resistance in Melon and Nonhost Plants

2004 ◽  
Vol 17 (6) ◽  
pp. 668-675 ◽  
Author(s):  
Juan A. Díaz ◽  
Cristina Nieto ◽  
Enrique Moriones ◽  
Verónica Truniger ◽  
Miguel A. Aranda
Keyword(s):  
Cdna Clone ◽  
Recessive Gene ◽  
Genomic Region ◽  
Open Reading Frames ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Gomphrena Globosa ◽  
Resistance Trait ◽  
Cucumis Melo L

Resistance of melon (Cucumis melo L.) to Melon necrotic spot virus (MNSV) is inherited as a single recessive gene, denoted nsv. No MNSV isolates described to date (e.g., MNSV-Mα5), except for the MNSV-264 strain described here, are able to overcome the resistance conferred by nsv. Analysis of protoplasts of susceptible (Nsv/-) and resistant (nsv/nsv) melon cultivars inoculated with MNSV-264 or MNSV-Mα5 indicated that the resistance trait conferred by this gene is expressed at the single-cell level. The nucleotide sequence of the MNSV-264 genome has a high nucleotide identity with the sequences of other MNSV isolates, with the exception of its genomic 3′-untranslated region (3′-UTR), where less than 50% of the nucleotides are shared between MNSV-264 and the other two MNSV isolates completely sequenced to date. Uncapped RNAs transcribed from a full-length MNSV-264 cDNA clone were infectious and caused symptoms indistinguishable from those caused by the parental viral RNA. This cDNA clone allowed generation of chimeric mutants between MNSV-264 and MNSV-Mα5 through the exchange of the last 74 nucleotides of their coat protein (CP) open reading frames and the complete 3′-UTRs. Analysis of protoplasts of susceptible and resistant melon cultivars inoculated with chimeric mutants clearly showed that the MNSV avirulence determinant resides in the exchanged region. The carboxy-termini of the CP of both isolates are identical; therefore, the avirulence determinant likely consists of the RNA sequence itself. We also demonstrated that this genomic region contains the determinant for the unique ability of the isolate MNSV-264 to infect noncucurbit hosts (Nicotiana benthamiana and Gomphrena globosa).

Resistance to Melon necrotic spot virus in Cucumis melo L. ‘Doublon’ artificially inoculated by the fungus vector Olpidium bornovanus

2006 ◽  
Vol 25 (5) ◽  
pp. 426-431 ◽  
Author(s):  
Cristina Mallor ◽  
Marisol Luis-Arteaga ◽  
José María Álvarez ◽  
Celia Montaner ◽  
Elena Floris
Keyword(s):  
Cucumis Melo ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Olpidium Bornovanus ◽  
Cucumis Melo L

A physical map covering the nsv locus that confers resistance to Melon necrotic spot virus in melon (Cucumis melo L.)

2005 ◽  
Vol 111 (5) ◽  
pp. 914-922 ◽  
Author(s):  
Mónica Morales ◽  
Gisella Orjeda ◽  
Cristina Nieto ◽  
Hans van Leeuwen ◽  
Amparo Monfort ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
Physical Map ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Cucumis Melo L

scholarly journals A Resistance to Systemic Symptom Expression of Melon Necrotic Spot Virus in Melon

2003 ◽  
Vol 128 (4) ◽  
pp. 541-547 ◽  
Author(s):  
Cristina Mallor ◽  
José María Álvarez ◽  
Marisol Luis-Arteaga
Keyword(s):  
Screening Program ◽  
Cell Movement ◽  
Recessive Gene ◽  
Systemic Symptom ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Systemic Development ◽  
Systemic Symptoms ◽  
Viral Distribution

Melon necrotic spot virus (MNSV) has been found affecting melon (Cucumis melo L.) crops. At present the only known resistance in melon is controlled by a single recessive gene, nsv. The presence of nsv in a melon genotype has been correlated with the lack of necrotic lesions on the mechanically inoculated cotyledons. Thus, in a screening program for MNSV resistance, melon genotypes that developed necrotic lesions in the inoculated cotyledons were discarded. However, in this paper we show that some melon accessions mechanically inoculated with MNSV do develop local necrotic lesions, therefore showing the absence of the gene nsv, but fail to develop the systemic symptoms typical of diseased plants under the screening conditions. In some of these accessions the influence of the temperature on the development of systemic symptoms was studied. The results showed that, depending on the accession, temperatures under 25 or 20 °C enhanced the systemic development of the disease. One of the tested varieties, `Doublon', did not develop systemic symptom at any of the tested temperatures (15, 17.5, 20, 22.5, 25, 27.5, and 30 °C). In this variety, the lack of systemic symptoms was correlated to the lack of virus infection of these tissues based upon ELISA results. MNSV was not detected in the uninoculated parts of the plant, and seems to remain confined to the local lesions produced on the cotyledons following the mechanical inoculation. Restriction of viral multiplication and/or cell-to-cell movement could explain the pattern of viral distribution in this variety. This reaction was observed in the `Doublon' plants mechanically inoculated with each of five isolates of MNSV tested, including an isolate that overcomes the nsv gene resistance.

Inheritance of resistance to systemic symptom expression of Melon necrotic spot virus (MNSV) in Cucumis melo L. `Doublon'

Euphytica ◽  
2003 ◽  
Vol 134 (3) ◽  
pp. 319-324 ◽  
Author(s):  
Cristina Mallor Giménez ◽  
José María Álvarez Álvarez ◽  
Marisol Luis Arteaga
Keyword(s):  
Cucumis Melo ◽  
Symptom Expression ◽  
Inheritance Of Resistance ◽  
Systemic Symptom ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Cucumis Melo L

Detection of Melon necrotic spot virus in water samples and melon plants by molecular methods

2003 ◽  
Vol 113 (2) ◽  
pp. 87-93 ◽  
Author(s):  
B Gosalvez ◽  
J.A Navarro ◽  
A Lorca ◽  
F Botella ◽  
M.A Sánchez-Pina ◽  
...  
Keyword(s):  
Water Samples ◽  
Molecular Methods ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus

Melon necrotic spot virus. [Distribution map].

2010 ◽  
Author(s):  
Keyword(s):  
Cucumis Melo ◽  
Citrullus Lanatus ◽  
Necrotic Spot ◽  
Distribution Map ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Olpidium Bornovanus ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is given for Melon necrotic spot virus (Tombusviridae: Carmovirus). The hosts include melon (Cucumis melo), cucumber (Cucumis sativus) and watermelon (Citrullus lanatus). Information is given on the geographical distribution in Europe (France, Greece, Crete, Italy, Sardinia, Netherlands, Norway, Spain, Canary islands, mainland Spain, Sweden, UK, England and Wales), Asia (China, Jiangsu, Iran, Israel, Japan, Hokkaido, Honshu, Kyushu, Shikoku, Korea Republic, Syria and Turkey), Africa (Tunisia), North America (Canada, Ontario, Mexico, USA, California) Central America and Caribbean (Guatemala, Honduras and Panama) and South America (Uruguay). The virus is transmitted by the fungal vector Olpidium bornovanus (syn. O. radicale) (Chytridiomycota: Olpidiaceae).

scholarly journals The structure of melon necrotic spot virus determined at 2.8 Å resolution

2007 ◽  
Vol 64 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Yasunobu Wada ◽  
Hideaki Tanaka ◽  
Eiki Yamashita ◽  
Chikako Kubo ◽  
Tamaki Ichiki-Uehara ◽  
...  
Keyword(s):  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus

A new cryptic virus belonging to the family Partitiviridae was found in watermelon co-infected with Melon necrotic spot virus

Virus Genes ◽  
2013 ◽  
Vol 47 (2) ◽  
pp. 382-384 ◽  
Author(s):  
Noa Sela ◽  
Oded Lachman ◽  
Victoria Reingold ◽  
Aviv Dombrovsky
Keyword(s):  
Necrotic Spot ◽  
Spot Virus ◽  
Cryptic Virus ◽  
Melon Necrotic Spot Virus ◽  
The Family
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