Plant tissue culture is becoming an important technique for the massive propagation of date palm and the available source lead to the success of this process. The mature female inflorescence is a novel and available source for in vitro propagation of date palm, nowadays become needed at the large scale to usage of organic nature materials in agriculture practice which are rich in plant growth regulators as well as micro elements. This study describes in vitro plant regeneration from mature female inflorescence explants of evaluating date palm (Phoenix dactylifera L.) by using the methanol extract of Moringa oleifera leaves which has great potential to be used as a natural source of nutraceutical in micropropagation. Mature inflorescences explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of an extract of Moringa leaves with or without add TDZ at 0.2 and 0.6 BA mg/l. Indoles, phenols, proteins, amino acids, and carbohydrates were determined. The addition of different concentrations of an extract of Moringa oleifera leaves with TDZ at 0.2 and 0.6 BA mg/l gave the highest significant average percentage of callus formation, friable callus, germination of embryos and numbers of embryos comparing with other treatments. Also, given the highest significant values of free amino acids and carbohydrates were noted in callus and embryo germination stage.
In vitro Morphogenesis of Arabian Date Palm (Phoenix dactylifera L.)