scholarly journals Isolation and Characterization of Hemolymph Clotting Factors in Drosophila melanogaster by a Pullout Method

2004 ◽  
Vol 14 (7) ◽  
pp. 625-629 ◽  
Author(s):  
Christoph Scherfer ◽  
Christine Karlsson ◽  
Olga Loseva ◽  
Gawa Bidla ◽  
Akira Goto ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Clotting Factors ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of the dopa decarboxylase gene of Drosophila melanogaster

1981 ◽  
Vol 1 (6) ◽  
pp. 475-485
Author(s):  
J Hirsh ◽  
N Davidson
Keyword(s):  
Drosophila Melanogaster ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Isolation Procedure ◽  
Chromosome Band ◽  
Dopa Decarboxylase ◽  
Developmental Profile ◽  
Isolation And Characterization

We have isolated chromosomal deoxyribonucleic acid clones containing the Drosophila dopa decarboxylase gene. We describe an isolation procedure which can be applied to other nonabundantly expressed Drosophila genes. The dopa decarboxylase gene lies within or very near polytene chromosome band 37C1-2. The gene is interrupted by at least one intron, and the primary mode of regulation is pretranslational. At least two additional sequences hybridized by in vivo ribonucleic acid-derived probes are found within a 35-kilobase region surrounding the gene. The developmental profile of ribonucleic acid transcribed from one of these regions differs from that of the dopa decarboxylase transcript.

Isolation and characterization of a mitochondrial RNA polymerase from Drosophila melanogaster

1987 ◽  
Vol 65 (2) ◽  
pp. 173-182 ◽  
Author(s):  
Michael Goldenthal ◽  
James T. Nishiura
Keyword(s):  
Drosophila Melanogaster ◽  
Rna Polymerase ◽  
Nuclear Dna ◽  
Gradient Centrifugation ◽  
Mitochondrial Rna ◽  
Mitochondrial Rna Polymerase ◽  
Isolation And Characterization ◽  
Cscl Gradient ◽  
Nuclear Rna

A DNA-dependent RNA polymerase was solubilized from sucrose gradient isolated, DNase-treated mitochrondria of Drosophila melanogaster. The isolated mitochondria were not detectably contaminated with nuclear DNA as shown by CsCl gradient centrifugation and polylysine Kieselguhr chromatography. The detergent-solubilized RNA polymerase was sensitive to rifampicin, resistant to α-amanitin, had an apparent molecular mass of about 60 kilodaltons, and displayed a tendency to aggregate, both in crude extracts or when purified. The mitochondrial RNA polymerase could be distinguished from nuclear RNA polymerases on the basis of size, salt optima, rifampicin sensitivity, and α-amanitin resistance.

Isolation and characterization of the Bactrocera oleae genes orthologous to the sex determining Sex-lethal and doublesex genes of Drosophila melanogaster

Gene ◽  
2005 ◽  
Vol 348 ◽  
pp. 111-121 ◽  
Author(s):  
Dimitrios Lagos ◽  
M. Fernanda Ruiz ◽  
Lucas Sánchez ◽  
Katia Komitopoulou
Keyword(s):  
Drosophila Melanogaster ◽  
Bactrocera Oleae ◽  
Isolation And Characterization ◽  
Sex Lethal

scholarly journals The Histone H3 Acetylase dGcn5 Is a Key Player in Drosophila melanogaster Metamorphosis

2005 ◽  
Vol 25 (18) ◽  
pp. 8228-8238 ◽  
Author(s):  
Clément Carré ◽  
Dimitri Szymczak ◽  
Josette Pidoux ◽  
Christophe Antoniewski
Keyword(s):  
Drosophila Melanogaster ◽  
Histone H3 ◽  
Noticeable Effect ◽  
Developmental Transitions ◽  
Isolation And Characterization ◽  
Lysine Residues ◽  
Developmental Role ◽  
Variant Protein

ABSTRACT Although it has been well established that histone acetyltransferases (HATs) are involved in the modulation of chromatin structure and gene transcription, there is only little information on their developmental role in higher organisms. Gcn5 was the first transcription factor with HAT activity identified in eukaryotes. Here we report the isolation and characterization of Drosophila melanogaster dGcn5 mutants. Null dGcn5 alleles block the onset of both oogenesis and metamorphosis, while hypomorphic dGcn5 alleles impair the formation of adult appendages and cuticle. Strikingly, the dramatic loss of acetylation of the K9 and K14 lysine residues of histone H3 in dGcn5 mutants has no noticeable effect on larval tissues. In contrast, strong cell proliferation defects in imaginal tissues are observed. In vivo complementation experiments revealed that dGcn5 integrates specific functions in addition to chromosome binding and acetylation. Surprisingly, a dGcn5 variant protein with a deletion of the bromodomain, which has been shown to recognize acetylated histones, appears to be fully functional. Our results establish dGcn5 as a major histone H3 acetylase in Drosophila which plays a key role in the control of specific morphogenetic cascades during developmental transitions.

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