Development and validation of a multiplex PCR assay for identification of the epidemic ST-258/512 KPC-producing Klebsiella pneumoniae clone

2014 ◽  
Vol 78 (1) ◽  
pp. 12-15 ◽  
Author(s):  
Amos Adler ◽  
Efrat Khabra ◽  
Inna Chmelnitsky ◽  
Panagiota Giakkoupi ◽  
Alkiviadis Vatopoulos ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Multiplex Pcr ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Development And Validation

scholarly journals Development of a multiplex PCR assay for identification of Klebsiella pneumoniae hypervirulent clones of capsular serotype K2

2014 ◽  
Vol 63 (12) ◽  
pp. 1608-1614 ◽  
Author(s):  
Suzanne Bialek-Davenet ◽  
Alexis Criscuolo ◽  
Florent Ailloud ◽  
Virginie Passet ◽  
Marie-Hélène Nicolas-Chanoine ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Multiplex Pcr ◽  
Sensu Stricto ◽  
Phylogenetic Group ◽  
Pcr Assay ◽  
Closely Related Species ◽  
Multiplex Pcr Assay ◽  
Genomic Analyses ◽  
Invasive Infections ◽  
Serotype K2

Hypervirulent Klebsiella pneumoniae isolates of capsular serotype K2 (hvKP-K2) that cause community-acquired invasive infections represent several unrelated clones, which all belong to phylogenetic group KpI. These clones can be recognized using multilocus sequence typing and genomic analyses, but no rapid method currently exists to differentiate them. In this work, a multiplex PCR assay was developed to identify three hvKP-K2 groups: (i) sequence type (ST)86; (ii) ST380 and ST679 (i.e. clonal group 380); and (iii) ST65 and ST375. A specific genetic marker, Kp50233, allowing K. pneumoniae sensu stricto (corresponding to phylogroup KpI) to be distinguished from closely related species, was included in the assay. This PCR assay will be useful in better defining the epidemiology and clinical features of emerging virulent K. pneumoniae clones.

Development and validation of a new one step Multiplex-PCR assay for the detection of ten Lactobacillus species

Anaerobe ◽  
2019 ◽  
Vol 59 ◽  
pp. 192-200
Author(s):  
Carlos Gaspar ◽  
Rita Palmeira-de-Oliveira ◽  
José Martinez-de-Oliveira ◽  
José das Neves ◽  
Paula G. Pestana ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Lactobacillus Species ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
One Step ◽  
Development And Validation

scholarly journals Multiplex PCR for Identification of Two Capsular Types in Epidemic KPC-Producing Klebsiella pneumoniae Sequence Type 258 Strains

2014 ◽  
Vol 58 (7) ◽  
pp. 4196-4199 ◽  
Author(s):  
Liang Chen ◽  
Kalyan D. Chavda ◽  
Jacqueline Findlay ◽  
Gisele Peirano ◽  
Katie Hopkins ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Multiplex Pcr ◽  
Capsular Polysaccharide ◽  
Sequence Type ◽  
Pcr Assay ◽  
Content Type ◽  
Multiplex Pcr Assay ◽  
Polysaccharide Synthesis ◽  
Sequence Types

ABSTRACTWe developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258]cps-1andcps-2) in epidemicKlebsiella pneumoniaeST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifyingcpstypes in 60 ST258K. pneumoniaesequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association betweencpstype andK. pneumoniaecarbapenemase (KPC) variant:cps-1is largely associated with KPC-2, whilecps-2is primarily associated with KPC-3.

scholarly journals Development and validation of a real-time multiplex PCR assay for the detection of dermatophytes and Fusarium spp.

2019 ◽  
Vol 68 (11) ◽  
pp. 1641-1648 ◽  
Author(s):  
Seok Hwee Koo ◽  
Yee Leng Teoh ◽  
Wei Liang Koh ◽  
Harumi Ochi ◽  
Sher Kye Tan ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Fusarium Spp ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Development And Validation

Development and validation of multiplex PCR assay for differentiating tunas and billfishes

2021 ◽  
Vol 30 (4) ◽  
pp. 497-503
Author(s):  
Na-Ye-Seul Kim ◽  
Eun-Ji Park ◽  
Seo-Hyun Lee ◽  
Kwang-Ho Mun ◽  
Ji-Young Yang ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Development And Validation

scholarly journals Development and validation of a multiplex-PCR assay for X-linked intellectual disability

2013 ◽  
Vol 14 (1) ◽  
Author(s):  
Paula Jorge ◽  
Bárbara Oliveira ◽  
Isabel Marques ◽  
Rosário Santos
Keyword(s):  
Intellectual Disability ◽  
Multiplex Pcr ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Development And Validation
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