“You cannot stay in the laboratory forever”*: Taking pig kidney xenotransplantation from the laboratory to the clinic

1975 ◽

Vol 33 ◽

pp. 458-459

Author(s):

W. Allen Shannon ◽

Hannah L. Wasserkrug ◽

andArnold M. Seligman

Keyword(s):

Guinea Pig ◽

Oxidase Activity ◽

Amine Oxidase ◽

Histochemical Demonstration ◽

Guinea Pig Heart ◽

Pig Kidney ◽

Cytoplasmic Vacuoles ◽

Liver And Kidney ◽

Mitochondrial Oxidation ◽

Amine Oxidase Activity

The synthesis of a new substrate, p-N,N-dimethylamino-β-phenethylamine (DAPA)3 (Fig. 1) (1,2), and the testing of it as a possible substrate for tissue amine oxidase activity have resulted in the ultracytochemical localization of enzyme oxidase activity referred to as DAPA oxidase (DAPAO). DAPA was designed with the goal of providing an amine that would yield on oxidation a stronger reducing aldehyde than does tryptamine in the histochemical demonstration of monoamine oxidase (MAO) with tetrazolium salts.Ultracytochemical preparations of guinea pig heart, liver and kidney and rat heart and liver were studied. Guinea pig kidney, known to exhibit high levels of MAO, appeared the most reactive of the tissues studied. DAPAO reaction product appears primarily in mitochondrial outer compartments and cristae (Figs. 2-4). Reaction product is also localized in endoplasmic reticulum, cytoplasmic vacuoles and nuclear envelopes (Figs. 2 and 3) and in the sarcoplasmic reticulum of heart.

Download Full-text

Development of an ex vivo model of pig kidney perfused with human lymphocytes. Analysis of xenogeneic cellular reactions

Surgery ◽

10.1067/msy.2000.107063 ◽

2000 ◽

Vol 128 (3) ◽

pp. 447-457 ◽

Cited By ~ 30

Author(s):

Bachir Khalfoun ◽

Didier Barrat ◽

Hervé Watier ◽

Marie Christine Machet ◽

Brigitte Arbeille-Brassart ◽

...

Keyword(s):

Ex Vivo ◽

Human Lymphocytes ◽

Ex Vivo Model ◽

Pig Kidney

Download Full-text

THE METABOLISM OF DIHYDROXYPHENYLALANINE BY GUINEA PIG KIDNEY EXTRACTS

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)56768-5 ◽

1949 ◽

Vol 179 (3) ◽

pp. 1037-1048

Author(s):

Robert E. Clegg ◽

Robert.Ridgely. Sealock

Keyword(s):

Guinea Pig ◽

Pig Kidney

Download Full-text

Isolation and Characterization of a Monoclonal Antibody against Pig Kidney Sodium- and Potassium-Activated ATPase1

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a135260 ◽

1985 ◽

Vol 98 (1) ◽

pp. 209-217 ◽

Cited By ~ 7

Author(s):

Osamu URAYAMA ◽

Hideaki NAGAMUNE ◽

Makoto NAKAO ◽

Yukichi HARA ◽

Hiroyuki SUGIYAMA ◽

...

Keyword(s):

Monoclonal Antibody ◽

Pig Kidney ◽

Isolation And Characterization ◽

Sodium And Potassium

Download Full-text

STUDIES ON MICROPEROXISOMES II. A CYTOCHEMICAL METHOD FOR LIGHT AND ELECTRON MICROSCOPY

Journal of Histochemistry & Cytochemistry ◽

10.1177/20.12.1006 ◽

1972 ◽

Vol 20 (12) ◽

pp. 1006-1023 ◽

Cited By ~ 144

Author(s):

ALEX B. NOVIKOFF ◽

PHYLLIS M. NOVIKOFF ◽

CLEVELAND DAVIS ◽

NELSON QUINTANA

Keyword(s):

Endoplasmic Reticulum ◽

Guinea Pig ◽

Lipid Droplets ◽

Smooth Endoplasmic Reticulum ◽

Light And Electron Microscopy ◽

Distal Tubule ◽

Striking Difference ◽

High Concentration ◽

Electron Microscopic ◽

Pig Kidney

A modification of the Novikoff-Goldfischer alkaline 3,3'-diaminobenzidine medium for visualizing peroxisomes is described. It makes possible light microscopic as well as electron microscopic studies of a recently described class of peroxisomes, the microperoxisomes. Potassium cyanide (5 x 10–3 M) is included in the medium to inhibit mitochondrial staining, the pH is 9.7 and there is a high concentration of H2O2 (0.05%). Two cell types have been chosen to illustrate the advantages of the new procedure for demonstrating the microperoxisomes: the absorptive cells in the human jejunum and the distal tubule cells in the guinea pig kidney. Suggestive relations of microperoxisomes and lipid are described in the human jejunum. The microperoxisomes are strategically located between smooth endoplasmic reticulum that radiates toward the organelles and contains lipid droplets and "central domains" of highly specialized endoplasmic reticulum which do not show the lipid droplets. The microperoxisomes are also present at the periphery of large lipid-like drops. In the guinea pig kidney tubule there is a striking difference between the thick limb of Henle and distal tubule. The distal tubule has a population of cells with large numbers of microperoxisomes readily visible by light microscopy; these cells are not present in the thick limb of Henle. Other differences between the two are also described.

Download Full-text