Spinal cord neurodegeneration after inorganic mercury long-term exposure in adult rats: Ultrastructural, proteomic and biochemical damages associated with reduced neuronal density

2020 ◽  
Vol 191 ◽  
pp. 110159 ◽  
Author(s):  
Márcio Gonçalves Corrêa ◽  
Leonardo Oliveira Bittencourt ◽  
Priscila Cunha Nascimento ◽  
Railson Oliveira Ferreira ◽  
Walessa Alana Bragança Aragão ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Inorganic Mercury ◽  
Adult Rats ◽  
Neuronal Density

Long-Term Exposure to Inorganic Mercury Leads to Oxidative Stress in Peripheral Blood of Adult Rats

2020 ◽  
Author(s):  
Victória dos Santos Chemelo ◽  
Leonardo Oliveira Bittencourt ◽  
Walessa Alana Bragança Aragão ◽  
Sávio Monteiro dos Santos ◽  
Renata Duarte Souza-Rodrigues ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Peripheral Blood ◽  
Inorganic Mercury ◽  
Adult Rats

scholarly journals A comprehensive study of long-term skeletal changes after spinal cord injury in adult rats

Bone Research ◽  
2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Tiao Lin ◽  
Wei Tong ◽  
Abhishek Chandra ◽  
Shao-Yun Hsu ◽  
Haoruo Jia ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Adult Rats ◽  
Skeletal Changes ◽  
Cord Injury ◽  
Comprehensive Study

Complete reorganization of the motor cortex of adult rats following long-term spinal cord injuries

2013 ◽  
Vol 38 (2) ◽  
pp. 2271-2279 ◽  
Author(s):  
Shashank Tandon ◽  
Niranjan Kambi ◽  
Hisham Mohammed ◽  
Neeraj Jain
Keyword(s):  
Spinal Cord ◽  
Motor Cortex ◽  
Spinal Cord Injuries ◽  
Adult Rats

Long-term exposure to lead reduces antioxidant capacity and triggers motor neurons degeneration and demyelination in spinal cord of adult rats

2020 ◽  
Vol 194 ◽  
pp. 110358 ◽  
Author(s):  
Dannilo Roberto Ferreira da Silva ◽  
Leonardo Oliveira Bittencourt ◽  
Walessa Alana Bragança Aragão ◽  
Priscila Cunha Nascimento ◽  
Luana Ketlen Reis Leão ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Antioxidant Capacity ◽  
Motor Neurons ◽  
Adult Rats

scholarly journals From Molecules to Behavior in Long-Term Inorganic Mercury Intoxication: Unraveling Proteomic Features in Cerebellar Neurodegeneration of Rats

2021 ◽  
Vol 23 (1) ◽  
pp. 111
Author(s):  
Leonardo Oliveira Bittencourt ◽  
Victória Santos Chemelo ◽  
Walessa Alana Bragança Aragão ◽  
Bruna Puty ◽  
Aline Dionizio ◽  
...  
Keyword(s):  
Purkinje Cells ◽  
Inorganic Mercury ◽  
Nervous System Development ◽  
Proteomic Profile ◽  
Mercury Toxicity ◽  
Adult Rats ◽  
Motor Functions ◽  
Proteomic Approach ◽  
Mercury Intoxication

Mercury is a severe environmental pollutant with neurotoxic effects, especially when exposed for long periods. Although there are several evidences regarding mercury toxicity, little is known about inorganic mercury (IHg) species and cerebellum, one of the main targets of mercury associated with the neurological symptomatology of mercurial poisoning. Besides that, the global proteomic profile assessment is a valuable tool to screen possible biomarkers and elucidate molecular targets of mercury neurotoxicity; however, the literature is still scarce. Thus, this study aimed to investigate the effects of long-term exposure to IHg in adult rats’ cerebellum and explore the modulation of the cerebellar proteome associated with biochemical and functional outcomes, providing evidence, in a translational perspective, of new mercury toxicity targets and possible biomarkers. Fifty-four adult rats were exposed to 0.375 mg/kg of HgCl2 or distilled water for 45 days using intragastric gavage. Then, the motor functions were evaluated by rotarod and inclined plane. The cerebellum was collected to quantify mercury levels, to assess the antioxidant activity against peroxyl radicals (ACAPs), the lipid peroxidation (LPO), the proteomic profile, the cell death nature by cytotoxicity and apoptosis, and the Purkinje cells density. The IHg exposure increased mercury levels in the cerebellum, reducing ACAP and increasing LPO. The proteomic approach revealed a total 419 proteins with different statuses of regulation, associated with different biological processes, such as synaptic signaling, energy metabolism and nervous system development, e.g., all these molecular changes are associated with increased cytotoxicity and apoptosis, with a neurodegenerative pattern on Purkinje cells layer and poor motor coordination and balance. In conclusion, all these findings feature a neurodegenerative process triggered by IHg in the cerebellum that culminated into motor functions deficits, which are associated with several molecular features and may be related to the clinical outcomes of people exposed to the toxicant.

scholarly journals Heterosynaptic long-term potentiation from the anterior cingulate cortex to spinal cord in adult rats

2018 ◽  
Vol 14 ◽  
pp. 174480691879840
Author(s):  
Qi-Yu Chen ◽  
Tao Chen ◽  
Li-Jun Zhou ◽  
Xian-Guo Liu ◽  
Min Zhuo
Keyword(s):  
Spinal Cord ◽  
Anterior Cingulate Cortex ◽  
Cingulate Cortex ◽  
Long Term Potentiation ◽  
Anterior Cingulate ◽  
Adult Rats ◽  
Term Potentiation

scholarly journals Mechanical Pain Hypersensitivity after Incisional Surgery Is Enhanced in Rats Subjected to Neonatal Peripheral Inflammation

2007 ◽  
Vol 106 (6) ◽  
pp. 1204-1212 ◽  
Author(s):  
Ya-Chun Chu ◽  
Kwok-Hon Chan ◽  
Mei-Yung Tsou ◽  
Su-Man Lin ◽  
Ying-Chou Hsieh ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Term Effect ◽  
Peripheral Inflammation ◽  
Adult Rats ◽  
Pain Hypersensitivity ◽  
Long Term Effect ◽  
Plantar Incision ◽  
Fos Expression ◽  
Incisional Pain

Background Neonatal pain and inflammation may lead to a long-term effect on nociceptive processing in adults. The current study examined the characteristics of postoperative incisional pain behaviors in adult rats that were subjected to neonatal peripheral inflammation. Methods Rat pups received a subcutaneous injection of saline or carrageenan into the plantar surface of the left hind paw at postnatal day 1. Naive pups were used as the control. Paw withdrawal thresholds to punctuate mechanical stimuli were examined at postnatal days 35, 42, and 49. After rats received a plantar incision on the left or right hind paw at postnatal day 50, paw withdrawal thresholds were measured at 4 h, 1 day, 2 days, 3 days, 5 days, and 7 days after incision. In addition, spinal cord Fos expression was detected at 2 h after incision. Finally, the effects of intrathecal N-methyl-D-aspartate receptor antagonists DL-2-amino-5-phosphonovaleric acid and dizocilpine and the nitric oxide synthase inhibitor L-N-nitro-arginine methylester on incisional pain were examined at 4 h after incision. Results Although the rats subjected to neonatal peripheral carrageenan injection developed mechanical hypoalgesia in bilateral hind paws at baseline, they displayed increased spinal cord Fos expression at 2 h and exaggerated mechanical pain hypersensitivity at 4 h (but not at other time points) after plantar incision. Intrathecal DL-2-amino-5-phosphonovaleric acid, dizocilpine, and L-N-nitro-arginine methylester significantly attenuated incision-induced mechanical pain hypersensitivity at 4 h after incision in the neonatally carrageenan-treated rats, but not in the naive or neonatally saline-treated rats. Conclusions The authors' results suggest that early inflammatory insults during the neonatal period could produce excessive incision-associated mechanical pain hypersensitivity in adult rats. Spinal cord N-methyl-D-aspartate receptors and downstream nitric oxide signaling might contribute to this abnormal pain hypersensitivity, although the mechanisms underlying the long-term effect of neonatal inflammation are still unclear.

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