It is an important basis for optimizing land spatial patterns and guiding land spatial planning to clarify the ecological conditions of land space, arrange reasonably agricultural production and urban-rural construction space. The network formed by green infrastructures has important ecological significance in maintaining the migration of biological species and ensuring the continuity of the ecological process. Based on the concept of ecological priority and green development, this paper constructs a framework of the land space development suitability evaluation based on green infrastructure evaluation. From the perspective of ecosystem service value and ecological sensitivity, the evaluation factors are selected to make a comprehensive evaluation of green infrastructure. The evaluation results are the basis for the evaluation of the importance of ecological protection, the suitability of agricultural production, and the suitability of construction. At last, taking Harbin as an example, the evaluation framework is applied to preliminarily determine the red line of ecological protection and the scope of ecological space, agricultural space, and urban-rural construction space in 2015. It is expected to provide a reference basis for the compilation of land and space planning in Harbin.