Morphological, immunohistochemical and chromosome in situ hybridization in the differential diagnosis of Hydatidiform Mole and Hydropic Abortion

2007 ◽  
Vol 135 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Mariana S. Maggiori ◽  
Luiz C. Peres
Keyword(s):  
Differential Diagnosis ◽  
In Situ Hybridization ◽  
Hydatidiform Mole ◽  
Hydropic Abortion

Sinonasal T-cell Lymphoma and Wegener's Granulomatosis: Aspects in Early Differential Diagnosis

1996 ◽  
Vol 10 (4) ◽  
pp. 239-246
Author(s):  
Anders Cervin ◽  
Michael Dictor ◽  
Olof Kalm
Keyword(s):  
Differential Diagnosis ◽  
In Situ Hybridization ◽  
T Cell ◽  
Wegener’S Granulomatosis ◽  
Cell Lymphoma ◽  
Wegener's Granulomatosis ◽  
T Cell Lymphoma ◽  
Upper Airways ◽  
Gastrointestinal Complications

The clinical course of 12 patients with sinonasal T-cell lymphoma retrospectively diagnosed using in situ hybridization for Epstein-Barr virus RNA was compared with that of 10 recently treated patients with Wegener's granulomatosis (WG) in the upper airways. In particular, we studied the presenting signs and symptoms of both diseases, which commonly offer a problem in differential diagnosis at the clinical and pathological level. A bimodal age distribution was suggested in both T-cell lymphoma and WG; five patients with T-cell lymphoma developed disease prior to 40 years of age. Four of the 12 lymphoma patients had a history of “chronic rhinitis” for several years before developing mucosal ulcerations, which were initially unilateral, as opposed to the bilateral ulcerations in early sinonasal WG. Two lymphoma patients had swelling of the nasal dorsum and cheek. In contrast to the WG patients, cases of T-cell lymphoma did not exhibit associated clinical signs of arthritis, conjunctivitis, pulmonary lesions, or nephritis in the early stage of the disease. Nine of the patients with T-cell lymphoma presenting as a sinonasal lesion developed disseminated disease, variably including infiltrates in intestine, lung, CNS, and skin. Four of these patients died from gastrointestinal complications of their disease. We conclude that unilateral ulcerative or hemorrhagic polypoid mucosal lesions in the sinonasal area are suggestive of lymphoma rather than WG, and nonspecific symptoms, at least in Western patients, may be present as early as the second or third decade of life. A biopsy specimen containing T lymphocytes positive for the EBV ribonucleoprotein EBER1 on in situ hybridization offers reliable confirmation of T-cell lymphoma and is of differential diagnostic value against WG.

Underexpression of 4 Placenta-Associated MicroRNAs in Complete Hydatidiform Moles

2012 ◽  
Vol 22 (6) ◽  
pp. 1075-1080 ◽  
Author(s):  
Quan Na ◽  
Dan Wang ◽  
Weiwei Song
Keyword(s):  
In Situ Hybridization ◽  
Hydatidiform Mole ◽  
Polymerase Chain Reaction Analysis ◽  
Normal Pregnancy ◽  
Differentially Expressed ◽  
Control Group ◽  
Differentially Expressed Mirnas ◽  
Hydatidiform Moles

ObjectiveSeveral placental microRNAs (miRNAs) have been identified as placenta-associated miRNAs with the potential of estimating the condition of the placenta. However, our understanding of these miRNAs is limited. The aim of this study was to determine the expression of 8 placenta-associated miRNAs (miR-512-3p, miR-517a, miR-517b, miR-518b, miR-519a, miR-1185, miR-1283, and miR-1323) in complete hydatidiform mole (CHM).MethodsSamples were obtained from patients with CHM (CHM group, n = 12) and elective terminations of normal pregnancy (control group, n = 20). We detected differentially expressed placenta-associated miRNAs in placenta by quantitative real-time reverse transcriptase–polymerase chain reaction analysis. Subsequently, we assessed the expression location of differentially expressed miRNAs by in situ hybridization analysis.ResultsFour placenta-associated miRNAs (miR-517a, miR-517b, miR-518b, and miR-519a) were underexpressed in the CHM group, compared with the control group (P < 0.01). When further investigating these 4 miRNAs with regard to in vivo localization by in situ hybridization, we found that 2 miRNAs (miR-517b and miR-518b) were detected exclusively in the trophoblast layer, with little signal (if any) observed in villous stroma cells.ConclusionsThe results show that 4 miRNAs (miR-517a, miR-517b, miR-518b, and miR-519a) are deregulated in CHM, which suggests the involvement of these miRNAs in the functions of CHM placenta.

Twin pregnancies with complete hydatidiform mole and coexisting fetus: Use of fluorescent in situ hybridization to evaluate placental X- and Y-chromosomal content

1995 ◽  
Vol 26 (11) ◽  
pp. 1175-1180 ◽  
Author(s):  
Sung R Choi-hong ◽  
David R Genest ◽  
Christopher P Crum ◽  
Ross Berkowitz ◽  
Donald P Goldstein ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Hydatidiform Mole ◽  
Complete Hydatidiform Mole ◽  
Twin Pregnancies

scholarly journals Utility of p57KIP2 and Her-2 Fluorescence in Situ Hybridization in Differentiating Partial from Complete Hydatidiform Mole

2016 ◽  
Vol 50 (4) ◽  
Author(s):  
Michele H. Diwa ◽  
Min-A Kim ◽  
Jose Maria C. Avila ◽  
David G. Pedroza ◽  
Michelle Anne M. Encinas-Latoy
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Hydatidiform Mole ◽  
Complete Hydatidiform Mole ◽  
Her 2

...

Primary intracranial dural-based synovial sarcoma with an unusual SYT fluorescence in situ hybridization pattern

2008 ◽  
Vol 109 (5) ◽  
pp. 897-903 ◽  
Author(s):  
Craig Horbinski ◽  
Kathleen Cieply ◽  
Ghassan K. Bejjani ◽  
Kathryn McFadden
Keyword(s):  
Differential Diagnosis ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Synovial Sarcoma ◽  
Spindle Cell ◽  
Second Primary ◽  
Hybridization Pattern ◽  
Molecular Studies

The authors present the case of an elderly man with a primary dural-based intracranial synovial sarcoma. Histological and immunohistochemical profiles of the lesion were diagnostic for a synovial sarcoma, and molecular studies using fluorescence in situ hybridization were compatible with a synovial sarcoma. A wide array of spindle cell neoplasms has been described as originating in the dura. To the authors' knowledge, however, this is only the second primary durabased intracranial synovial sarcoma ever reported, emphasizing the importance of a broad differential diagnosis when encountering spindle cell lesions of the meninges.

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