Enhancing the sustainability of KsdD as a biocatalyst for steroid transformation by immobilization on epoxy support

2021 ◽  
Vol 146 ◽  
pp. 109777 ◽  
Author(s):  
Shuhong Mao ◽  
Ying Chen ◽  
Jing Sun ◽  
Cancan Wei ◽  
Zhan Song ◽  
...  
Endocrinology ◽  
2000 ◽  
Vol 141 (5) ◽  
pp. 1587-1589 ◽  
Author(s):  
Wen-Chao Song ◽  
Michael H. Melner

1981 ◽  
Vol 11 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Toshiomi Yoshida ◽  
Masaki Sueki ◽  
Hisaharu Taguchi ◽  
Songsri Kulprecha ◽  
Naline Nilubol

1984 ◽  
Vol 30 (1) ◽  
pp. 57-62 ◽  
Author(s):  
J. Długoński ◽  
L. Sedlaczek ◽  
A. Jaworski

Protoplasts were obtained from Hyphoderma roseum (Fries) and Cunninghamella elegans (Lendner), fungi capable of steroid 11-hydroxylation. The lytic enzyme preparation was derived from Trichoderma viride CBS 354-33. Homogeneous protoplast suspension, free of mycelial debris and cell wall fragments, transformed cortexolone and 6α-fluorocortexolone-16,17-acetonide to the same products as the intact mycelium of the microorganisms. Liberation of protoplasts and their stabilizaiton during steroid transformation was the most effective in 0.8 M MgSO4; still, this compound impaired steroid hydroxylation. Consequently, the concentration of the transformation product formed was nearly the same as in sucrose, mannitol, and sorbitol, compounds which caused no inhibition but which were less effective stabilizers.


1955 ◽  
Vol 3 (6) ◽  
pp. 372-374
Author(s):  
Eugene L. Dulaney ◽  
W. J. McAleer ◽  
H. R. Barkemeyer ◽  
Charles Hlavac

1980 ◽  
Vol 3 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Doo Ha Kim ◽  
S. B. Lee ◽  
Dewey D. Y. Ryu

1992 ◽  
Vol 8 (4) ◽  
pp. 399-401 ◽  
Author(s):  
P. K. Roy ◽  
A. W. Khan ◽  
J. Kumar ◽  
S. D. K. Chopra ◽  
S. K. Basu

Sign in / Sign up

Export Citation Format

Share Document