Approximate Models of Microbiological Processes in a Biofilm Formed on Fine Spherical Particles

This paper concerns the dynamical modeling of the microbiological processes that occur in the biofilms that are formed on fine inert particles. Such biofilm forms e.g. in fluidized-bed bio-reactors, expanded bed biofilm reactors and biofilm air-lift suspension reactors. An approximate model that is based on the Laplace–Carson transform and a family of approximate models that are based on the concept of the pseudo-stationary substrate concentration profile in the biofilm were proposed. The applicability of the models to the microbiological processes was evaluated following Monod or Haldane kinetics in the conditions of dynamical biofilm growth. The use of approximate models significantly simplifies the computations compared to the exact one. Moreover, the stiffness that was present in the exact model, which was solved numerically by the method of lines, was eliminated. Good accuracy was obtained even for large internal mass transfer resistances in the biofilm. It was shown that significantly higher accuracy was obtained using one of the proposed models than that which was obtained using the previously published approximate model that was derived using the homotopy analysis method.

The use of air-lift adsorber with a floating filling from a cross-linked chitosan hydrogels for Reactive Black 5 removal

This work substantially extends knowledge on the possibilities of treating colored industrial wastewater via sorption under flow conditions. The presented study aimed to determine the effectiveness of Reactive Black 5 (RB5) dye sorption from aqueous solutions under dynamic (flow) conditions in an unconventional air-lift type loop reactor with a filling made of hydrogel chitosan sorbents. The dye was removed from mono-component solutions (deionized water + RB5) and synthetic dyeing wastewater containing RB5 dye, NaCl (3 g/L), and an anti-creasing agent—UNICREASE JET (2 g/L). The sorbents tested in the study included: unmodified chitosan (CHs), chitosan ionically cross-linked with sodium citrate (CHs-CIT), and chitosan covalently cross-linked with epichlorohydrin (CHs-ECH). Each experimental series aimed to determine: the bed break-through time (CE = 0.1 C0), time of depletion of the sorbent’s sorption properties (CE = C0), and maximal sorption capacity of the sorbents (Qmax). The data obtained under dynamic conditions were described using Thomas, Yoon–Nelson, and Bohart–Adams models. The volume of the solution effectively treated in the air-lift reactor was significantly affected by chitosan sorbent type. At C0 = 50 mg RB5/L, the adsorber with the filling made of 1 g d.m. CHs allowed for the effective treatment of 4.6 L of synthetic wastewater (Qmax = 1504.7 mg/g), whereas CHs-ECH ensured 34.6 L of the treated solution (Qmax = 3212.9 mg/g).

Experimental Study on Void Fractions and Pressure Drops in Three-Phase Flow for Deep Sea Mining

Subsea minerals exist in the deep water within Japan’s exclusive economic zone. There are many technical issues which should be addressed for subsea mining. The air-lift pumping systems are one of promising methods for subsea minerals transport. Flow assurance for three-phase flow is important to design the air-lift pumping system for subsea mining. It is important to establish methods for estimating void fractions and frictional pressure drops. To establish the methods for three-phase flow, we reviewed previous studies for two- or three-phase flow. There are some models to estimate the void fractions such as slip flow model and drift flux model. There are also some models to estimate the frictional pressure drops such as homogeneous model and separated flow model. We calculated void fractions and frictional pressure drops by existing correlation and compared calculated results with experimental data in two- or three-phase flow. In addition, we proposed the methods for estimating the void fractions and frictional pressure drops in three-phase flow. These had fewer number of experimental constants than existing correlations, these could calculate void fractions and frictional pressure drops in more various conditions than existing correlations.

The Persistent Challenge of Pneumocystis Growth Outside the Mammalian Lung: Past and Future Approaches

The pathogenic fungi in the genus, Pneumocystis, have eluded attempts to continuously grow them in an ex vivo cultivation system. New data from transcriptomic and genomic sequencing studies have identified a myriad of absent metabolic pathways, helping to define their host obligate nature. These nutrients, factors, and co-factors are acquired from their mammalian host and provide clues to further supplementation of existing media formulations. Likewise, a new appreciation of the pivotal role for the sexual cycle in the survival and dissemination of the infection suggests that Pneumocystis species are obligated to undergo mating and sexual reproduction in their life cycle with a questionable role for an asexual cycle. The lack of ascus formation in any previous cultivation attempts may explain the failure to identify a sustainable system. Many characteristics of these ascomycetes suggest a biotrophic existence within the lungs of the mammalian hosts. In the present review, previous attempts at growing these fungi ex vivo are summarized. The significance of their life cycle is considered, and a list of potential supplements based on the genomic and transcriptomic studies is presented. State of the art technologies such as metabolomics, organoids, lung-on-a chip, and air lift cultures are discussed as potential growth systems.

RESEARCH RESULTS ON IMPROVEMENT OF THE JUSTIFICATION METHODOLOGY OF THE PARAMETERS OF A PNEUMATIC VACUUM (AIRLIFT) PUMP UNIT

The article discusses the methodology for substantiating the parameters and determining the technological and technical parameters of a pneumatic vacuum (air-lift) pump unit. The technology of water lifting from wells using a pneumatic vacuum (airlift) pumping unit for water supply of peasant and farm households has been substantiated. According to the results of theoretical and experimental studies, the optimal technological parameters are given: overpressure of compressed air created by the compressor at optimal heights of water lifting; specific gravity of the lifted water-air mixture; pump unit discharge Q; power consumption and efficiency of the pumping system and pump unit, water lifting height Н, diametrical dimension of the pump part DНЧ, permissible salinity and content of solid particles (sand) in it Мд, ambient air temperature for normal operation of the pump unit ТНР.