Immobilization of a commercial Aspergillus aculeatus enzyme preparation with fructosyltransferase activity in chitosan beads: A kinetic/thermodynamic study and fructo-oligosaccharides continuous production in enzymatic reactor

In this work, we developed a facile flow injection method to fabricate chitosan beads of uniform size in a continuous manner and assessed their properties as hemostatic dressings.

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Continuous production of galacto-oligosaccharides from lactose by Bullera singularis β-galactosidase immobilized in chitosan beads

Process Biochemistry ◽

10.1016/s0032-9592(98)00045-4 ◽

1998 ◽

Vol 33 (8) ◽

pp. 787-792 ◽

Cited By ~ 68

Author(s):

Huyn-Jae Shin ◽

Jong-Moon Park ◽

Ji-Won Yang

Keyword(s):

Continuous Production ◽

Chitosan Beads

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Kinetic behavior of soluble Pectinex Ultra SP-L converting sucrose into fructo-oligosaccharides in batch and continuous fashion

Progress in Agricultural Engineering Sciences ◽

10.1556/446.2020.00010 ◽

2020 ◽

Vol 16 (1) ◽

pp. 81-97

Author(s):

Zoltán Kovács ◽

Juraj Števek ◽

Miroslav Fikar ◽

Peter Czermak

Keyword(s):

Batch Reactor ◽

Continuous Production ◽

Value Added ◽

Batch Process ◽

Reaction Conditions ◽

Aspergillus Aculeatus ◽

A Value ◽

Industrial Implementation ◽

Production Scheme ◽

Experimental Runs

AbstractThe enzymatic conversion of sucrose to fructo-oligosaccharides (FOS) catalyzed by Pectinex Ultra SP-L, a commercial enzyme preparation from Aspergillus aculeatus, under free condition was studied. A mathematical analysis of the transfructosylation reactions was carried out to estimate the dynamic and steady-state performance of an enzyme membrane reactor (EMR) and to compare the continuous production scheme with the traditional batch process realized in stirred-tank reactor (STR). Kinetic parameters were identified simultaneously from a series of progress curves obtained from STR and EMR experimental runs. Model estimates appeared to fit well to experimental observations under the studied reaction conditions. Although conventional batch reactor outperforms EMR in terms of conversion, EMR compares favorably regarding productivity. The on-site industrial implementation of this technology might be attractive for food manufacturers aiming at utilizing a value-added sweetener mixture with prebiotic properties.

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Continuous Production of Galacto-Oligosaccharides by an Enzyme Membrane Reactor Utilizing Free Enzymes

Membranes ◽

10.3390/membranes10090203 ◽

2020 ◽

Vol 10 (9) ◽

pp. 203

Author(s):

Teng Cao ◽

Melinda Pázmándi ◽

Ildikó Galambos ◽

Zoltán Kovács

Keyword(s):

Enzyme Preparation ◽

Continuous Production ◽

Extended Period ◽

Degree Of Polymerization ◽

Enzyme Inactivation ◽

Membrane Reactors ◽

Feed Concentration ◽

Total Carbohydrate ◽

Significant Deterioration ◽

Recirculation Flow

Galacto-oligosaccharides (GOS) are prebiotic compounds widely used for their health-promoting effects. Conventionally, GOS is produced by the enzymatic conversion of lactose in stirred tank reactors (STR). The high operational costs associated with enzyme inactivation and removal might be reduced by the application of enzyme membrane reactors (EMR). In this study, we aimed to assess the potential of continuous GOS production by EMR using soluble Biolacta N5, a Bacillus circulans-derived commercial enzyme preparation. The steady-state performance of the EMR equipped with an ultrafiltration module was investigated as function of residence time (1.1–2.8 h) and enzyme load (17–190 U·g−1) under fixed operational settings of temperature (50 °C), pH (6.0), lactose feed concentration (300 g·kg−1), and recirculation flow-rate (0.18 m3·h−1). Results indicate that the yield of oligosaccharides with higher degree of polymerization (DP3-6) in STR (approx. 38% on total carbohydrate basis) exceeds that measured in EMR (ranging from 24% to 33%). However, a stable catalytic performance without a significant deterioration in product quality was observed when operating the EMR for an extended period of time (>120 h). Approx. 1.4 kg of DP3-6 was produced per one gram of crude enzyme preparation over the long-term campaigns, indicating that EMR efficiently recovers enzyme activity.

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