Phylogeny of Isatis (Brassicaceae) and allied genera based on ITS sequences of nuclear ribosomal DNA and morphological characters

During an investigation of lignicolous ascomycetes from Luxembourg, we isolated a Phialophora -like species that produced striking red colonies. To confirm the identity of this fungus as Catenulifera rhodogena , we compared it with isolates of Catenulifera rhodogena and Hyphodiscus hymeniophilus on a variety of media. Portions of the β-tubulin gene and the nuclear ribosomal DNA cistron (internal transcribed spacers (ITS) and large subunit (LSU)) were sequenced to examine the relationship of isolates of Catenulifera rhodogena and Hyphodiscus hymeniophilus from different substrates and to test the hypothesis that Cadophora and Catenulifera are congeneric. The phylogenetic position of Catenulifera within the Ascomycota was investigated based on the analysis of the small-subunit (SSU) rDNA sequences. The isolates examined were indistinguishable micromorphologically and closely related phylogenetically. Three strains of Catenulifera rhodogena from bark or wood and one strain from Piptoporus betulinus formed a strongly supported clade in analyses of β-tubulin and ITS sequences. This clade did not encompass the ex-type isolates of Cistella rubescens and Scopulariopsis rhodogena or a second isolate of Catenulifera rhodogena from Piptoporus betulinus. Analysis of partial LSU sequences confirmed the close phylogenetic relationship of Catenulifera rhodogena and Hyphodiscus hymeniophilus but provided no evidence that the isolates could be grouped by substrate or that Catenulifera is synonymous with Cadophora. The position of Catenulifera within the Helotiales was not resolved based on the comparison of LSU and SSU sequences, but the isolate for which we obtained complete SSU sequence grouped with the root endophyte Phialocephala fortinii. Comparison of ITS sequences confirmed the close phylogenetic relationship of Hyphodiscus to members of the Dermateaceae and Hyaloscyphaceae.

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Phylogenetic relationship of some Onobrychis taxa naturally grown in Turkey based on morphology and nuclear ribosomal DNA ITS Sequences

Legume Research - An International Journal ◽

10.18805/lr.v0iof.11184 ◽

2016 ◽

Author(s):

Süleyman AVCI ◽

Nilgün Tekin ◽

Cengiz SANCAK ◽

Sebahattin ÖZCAN ◽

Amir Orojpour Marangi

Keyword(s):

Ribosomal Dna ◽

Clustering Analysis ◽

Molecular Data ◽

Its Sequences ◽

Nuclear Ribosomal Dna ◽

Morphological Data ◽

Nrdna Its ◽

Neighbor Joining ◽

Quantitative Characters ◽

Relationship Of

Phylogenetic relationships were studied based on morphology and nuclear ribosomal DNA internal transcript spacer (nrDNA ITS) sequences in 49 populations of 36 Onobrychis taxa belonging to 5 sections (Dendobrychis, Lophobrychis, Onobrychis, Heliobrychis and Hymenobrychis) naturally grown in Turkey. The morphological data (46 quantitative characters) were standardized and submitted for PCA analysis before the performance of hierarchical clustering analysis. In addition, a 577-bp region of the nrDNA ITS sequences was compared to generate a dendrogram using the neighbor-joining method. According to the obtained results, the Onobrychis genus is clearly divided into the Onobrychis and Sisyrosema subgenera both molecularly and morphologically; however, in terms of molecular data, while the Onobrychis subgenus showed polyphyletic features, the Sisyrosema subgenus had monophyletic features. Additionally, long-wing taxa belonging to Dendobrychis and Onobrychis and O. crista-galli belonging to Lophobrychis located in the same clade in Onobrychis showed polyphyletic features. This finding was confirmed in terms of long-wing taxa as well as morphology and indicated that these three sections were entangled with each other under the Onobrychis subgenus.

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