The Nitrone Spin Trap DMPO Prevents Lipopolysaccharide-induced Pro-inflammatory Programming of Macrophages

The cathodically and photochemically induced decomposition of thioazo compounds XC6H4-N2-S-C6H4CH3 and their polymers with X = NO2, COOH, and SO3H were investigated. The formation of carbon-centered XC6H4. and sulfur-centered .S-C6H4Y radicals was confirmed using spin-trap technique. These reactive radicals either abstract hydrogen from CH3CN solvent molecule forming .CH2CN radical or they recombine to cage products XC6H4-S-C6H4CH3 eliminating N2. The decomposition rate of the investigated thioazo compounds is characterized by a formal half-life time of 5 to 10 s.

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Mechanism of singlet oxygen interaction with spin trap α-phenyl-N-tert-butylnitrone

Journal of Photochemistry ◽

10.1016/0047-2670(84)85046-7 ◽

1984 ◽

Vol 26 (4) ◽

pp. 269-274 ◽

Cited By ~ 8

Author(s):

Alexander P. Darmanyan ◽

Galina Móger

Keyword(s):

Singlet Oxygen ◽

Spin Trap ◽

Oxygen Interaction

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Irradiated nifedipine—a nitroso spin trap

Magnetic Resonance in Chemistry ◽

10.1002/mrc.1260320612 ◽

1994 ◽

Vol 32 (6) ◽

pp. 370-372 ◽

Cited By ~ 4

Author(s):

Andrej Staško ◽

Vladimir Mišík ◽

Ladislav Omelka

Keyword(s):

Spin Trap

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Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

Journal of Toxicology ◽

10.1155/2011/973172 ◽

2011 ◽

Vol 2011 ◽

pp. 1-11

Author(s):

Shinobu Ito ◽

Tomohisa Mori ◽

Hideko Kanazawa ◽

Toshiko Sawaguchi

Keyword(s):

Free Radicals ◽

Electron Spin Resonance ◽

Electron Spin ◽

Spin Trap ◽

Detection Sensitivity ◽

Mouse Tissue ◽

Spin Adduct ◽

Simple Method ◽

Oxidation Stress ◽

Spin Resonance

Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗in liver (y=113.8–40.7 log (day),R1=-0.779,R2=0.6,P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.

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