Evaluation of parentage testing accuracy of child trafficking cases: Combining the exclusion probability and likelihood ratio approaches

The numbat (Myrmecobius fasciatus) is an endangered and peculiar marsupial with a diet that consists almost exclusively of termites. This study developed a parentage-testing system for numbats using microsatellite markers. Nineteen loci detected 143 alleles, with 4–13 alleles/locus and average expected heterozygosity of 77% (range 0.665–0.855). The total parentage exclusion probability was >0.9999 (given only the genotype of the offspring), >0.9999 for excluding a candidate parent from the parentage of an arbitrary offspring (given the genotype of the offspring and parent) and the probability of identity for full-sibs was 4.6×10–9. Overall, these microsatellites offer a simple and highly informative marker-set for parentage identification in numbats.

Download Full-text

Looking for breed differentiating SNP loci and for a SNP set for parentage testing in Mangalica

Archives Animal Breeding ◽

10.7482/0003-9438-56-019 ◽

2013 ◽

Vol 56 (1) ◽

pp. 200-207 ◽

Cited By ~ 2

Author(s):

A. Zsolnai ◽

G. Tóth ◽

J. Molnár ◽

V. Stéger ◽

F. Marincs ◽

...

Keyword(s):

Microsatellite Markers ◽

Whole Genome ◽

Parentage Testing ◽

Exclusion Probability

Abstract. The whole genome of Mangalica animals has been screened on the Illumina porcine chip giving the possibility (1) to replace the previously applied ten microsatellite markers by nine SNP loci to classify the Blond, Swallow-Belly and Red Mangalica individuals into three different breed groups (P>0.95); (2) to propose 54 SNP loci for parentage testing in Mangalica pigs where the exclusion probability is 0.999115 if one parent is known and the probability of identity is 1.54×10-23.

Download Full-text

Microsatellite panels suggested for parentage testing in cattle: informativeness revealed in Finnish Ayrshire and Holstein-Friesian populations (Research Note)

Agricultural and Food Science ◽

10.23986/afsci.5625 ◽

1999 ◽

Vol 8 (3) ◽

pp. 233-237 ◽

Cited By ~ 6

Author(s):

P. BREDBACKA ◽

M.T. KOSKINEN

Keyword(s):

Microsatellite Markers ◽

International Society ◽

Artificial Insemination ◽

Routine Testing ◽

Parentage Testing ◽

Low Level ◽

Exclusion Probability ◽

Holstein Friesian ◽

Core Set ◽

Parentage Control

Informativeness of eleven microsatellite markers suggested for parentage control in cattle by the International Society for Animal Genetics (ISAG) was studied in Finnish Ayrshire and Holstein-Friesian populations. Calculations were based on a sample of 100 non-sib artificial insemination bulls. Assuming one known parent the nine loci suggested for routine testing exhibited exclusion probabilities of 99.84% in the Ayrshires and 99.91% in the Holstein-Friesians. The addition of markers INRA23 and TGLA53, recommended for further investigations, increased the attained values to 99.94% in Ayrshires and to 99.98% in Holstein-Friesians. The recommended core set of six microsatellites provided a combined exclusion probability of 98.25% in Ayrshires and 99.32% in Holstein-Friesians. Although the combined values were high in general, a relatively low level of polymorphism was detected in some instances.;

Download Full-text

Evaluation of parentage testing in the Czech population of Holsteincattle

Czech Journal of Animal Science ◽

10.17221/3970-cjas ◽

2011 ◽

Vol 51 (No. 12) ◽

pp. 503-509 ◽

Cited By ~ 6

Author(s):

V. Řehout ◽

E. Hradecká ◽

J. Čítek

Keyword(s):

Parentage Testing ◽

Czech Population ◽

Exclusion Probability ◽

Holstein Calves

A set of 233 Holstein calves, their 233 dams and 44 sires from 4 large-sized herds was genotyped for 10 microsatellites recommended by ISAG for paternity/parentage testing. Heterozygosity of microsatellites ranged from 0.607 (SPS115) to 0.835 (TGLA227), and PIC from 0.575 (SPS115) to 0.816 (TGLA227) confirming a high polymorphism of each analysed locus. Their combined exclusion probability reached 0.999, which made them suitable and sufficiently accurate for parentage testing. A conflict between putative parents and calf in at least 2 markers with combined exclusion probability > 0.9 was required to reject parentage. The pedigree was considered incorrect in 25 (10.73%) out of the evaluated progeny/parent trios, of which in 10 samples the genotype of both parents did not match their offspring, and in 2 samples the putative dam was in conflict with the calf genotype. This result shows that the interchange of calves on farms with large-sized herds plays the role as important as the errors in sire identification, or recording mistakes.  

Download Full-text