scholarly journals Evaluation of parentage testing in the Czech population of Holsteincattle

2011 ◽  
Vol 51 (No. 12) ◽  
pp. 503-509 ◽  
Author(s):  
V. Řehout ◽  
E. Hradecká ◽  
J. Čítek

A set of 233 Holstein calves, their 233 dams and 44 sires from 4 large-sized herds was genotyped for 10 microsatellites recommended by ISAG for paternity/parentage testing. Heterozygosity of microsatellites ranged from 0.607 (SPS115) to 0.835 (TGLA227), and PIC from 0.575 (SPS115) to 0.816 (TGLA227) confirming a high polymorphism of each analysed locus. Their combined exclusion probability reached 0.999, which made them suitable and sufficiently accurate for parentage testing. A conflict between putative parents and calf in at least 2 markers with combined exclusion probability > 0.9 was required to reject parentage. The pedigree was considered incorrect in 25 (10.73%) out of the evaluated progeny/parent trios, of which in 10 samples the genotype of both parents did not match their offspring, and in 2 samples the putative dam was in conflict with the calf genotype. This result shows that the interchange of calves on farms with large-sized herds plays the role as important as the errors in sire identification, or recording mistakes.  

2020 ◽  
Vol 42 (2) ◽  
pp. 240
Author(s):  
Peter B. S. Spencer ◽  
Serina McConnell ◽  
Diana Prada ◽  
J. A. (Tony) Friend

The numbat (Myrmecobius fasciatus) is an endangered and peculiar marsupial with a diet that consists almost exclusively of termites. This study developed a parentage-testing system for numbats using microsatellite markers. Nineteen loci detected 143 alleles, with 4–13 alleles/locus and average expected heterozygosity of 77% (range 0.665–0.855). The total parentage exclusion probability was >0.9999 (given only the genotype of the offspring), >0.9999 for excluding a candidate parent from the parentage of an arbitrary offspring (given the genotype of the offspring and parent) and the probability of identity for full-sibs was 4.6×10–9. Overall, these microsatellites offer a simple and highly informative marker-set for parentage identification in numbats.


2013 ◽  
Vol 56 (1) ◽  
pp. 200-207 ◽  
Author(s):  
A. Zsolnai ◽  
G. Tóth ◽  
J. Molnár ◽  
V. Stéger ◽  
F. Marincs ◽  
...  

Abstract. The whole genome of Mangalica animals has been screened on the Illumina porcine chip giving the possibility (1) to replace the previously applied ten microsatellite markers by nine SNP loci to classify the Blond, Swallow-Belly and Red Mangalica individuals into three different breed groups (P>0.95); (2) to propose 54 SNP loci for parentage testing in Mangalica pigs where the exclusion probability is 0.999115 if one parent is known and the probability of identity is 1.54×10-23.


1999 ◽  
Vol 8 (3) ◽  
pp. 233-237 ◽  
Author(s):  
P. BREDBACKA ◽  
M.T. KOSKINEN

Informativeness of eleven microsatellite markers suggested for parentage control in cattle by the International Society for Animal Genetics (ISAG) was studied in Finnish Ayrshire and Holstein-Friesian populations. Calculations were based on a sample of 100 non-sib artificial insemination bulls. Assuming one known parent the nine loci suggested for routine testing exhibited exclusion probabilities of 99.84% in the Ayrshires and 99.91% in the Holstein-Friesians. The addition of markers INRA23 and TGLA53, recommended for further investigations, increased the attained values to 99.94% in Ayrshires and to 99.98% in Holstein-Friesians. The recommended core set of six microsatellites provided a combined exclusion probability of 98.25% in Ayrshires and 99.32% in Holstein-Friesians. Although the combined values were high in general, a relatively low level of polymorphism was detected in some instances.;


1992 ◽  
Vol 05 (03) ◽  
pp. 100-103 ◽  
Author(s):  
G. Jean ◽  
J. K. Roush ◽  
R. M. DeBowes ◽  
E. M. Gaughan ◽  
J. Kirpensteijn

SummaryThe holding power and holding power per mm bone width of 4.5 mm and 5.5 mm cortical and 6.5 mm cancellous orthopaedic screws were obtained by tensile load-to-failure studies in excised metacarpal and metatarsal bones of young female Holstein calves. Holding power and holding power per mm bone width of 6.5 mm orthopaedic screws were significantly greater than those of 4.5 and 5.5 mm orthopaedic screws in the diaphysis and metaphysis. Significant differences were not detected between holding power and holding power per mm bone width of 4.5 and 5.5 mm orthopaedic screws. The holding power was not different between metacarpi and metatarsi. The limiting factor in all tests of holding power was the shear strength of the bone. We found that 6.5 mm orthopaedic screws have the greatest holding power in the metacarpal and metatarsal bones of young calves.This study compares the holding power of 4.5 mm and 5.5 mm cortical and 6.5 mm cancellous orthopaedic screws in excised metacarpal and metatarsal bones from young female Holstein calves. We found that 6.5 mm orthopaedic screws have the greatest holding power.


Kontakt ◽  
2020 ◽  
Vol 22 (1) ◽  
pp. 16-26 ◽  
Author(s):  
Sylva Bártlová ◽  
Valérie Tóthová ◽  
Ivana Chloubová ◽  
Lenka Šedová ◽  
Věra Olišarová ◽  
...  

2019 ◽  
pp. 32-37
Author(s):  
L.N. BAKAEVA ◽  
A.S. KARAMAEVA ◽  
S.V. KARAMAEV

Работа  посвящена изучению особенностей формирования иммунитета у новорожденных телят. Объектом исследований служили коровы бестужевской, черно-пестрой, голштинской, айрширской пород и их потомство, разводимые в природно-климатической зоне Среднего Поволжья и Южного Урала. Установлено, что за последний месяц перед отелом в сыворотке крови коров происходит снижение содержания иммуноглобулинов, у бестужевской породы на 19,3%, черно-пестрой — на 15,8%, голштинской — на 13,4%, айрширской — на 15,0%. Это обусловлено тем, что иммуноглобулины интенсивно перемещаются в клетки секреторного эпителия альвеолярной ткани вымени. В молозиве первого удоя содержится от 57,86 г/л (голштинская порода) до 106,78 г/л (бестужевская порода) иммуноглобулинов. В молозиве второго удоя содержание иммуноглобулинов снижается у бестужевской породы на 18,9%, черно-пестрой — на 25,1%, голштинской — на 29,3%, айрширской — на 21,7%, а при четвертом доении, соответственно, на 45,4; 51,6; 52,4; 49,2%. Через 6 ч после первого выпаивания молозивом, содержание иммуноглобулинов в сыворотке крови телят всех пород, за исключением голштинской, было выше 10 мг/мл, что считается физиологической нормой. Исследования показали, что в каждой породе есть телята, не воспринимающие иммуноглобулины из молозива матери, доля которых составляет, соответственно 20; 16; 30; 10%. Наиболее опасными для здоровья теленка являются первые 5 дней жизни. В этот период в бестужевской породе заболело 6% телят, черно-пестрой — 24, голштинской — 42, айрширской — 14%. Всего за первый месяц жизни заболеваемость телят в группах составила, соответственно, 16; 48; 76; 26%.The article presents the study of peculiarities of immune development in newborn calves. The objects of interest were Bestuzhev, Black-and-White, Holstein and Ayrshire cows and their progeny raised in the environmental and climatic zone of Middle Volga and Southern Ural. The study has shown that during the last month prior to calving there was a decrease of immunoglobulin content in the cows’ blood serum: in Bestuzhev cows by 19.3%, in Black-and-White cows by 15.8%, in Holstein cows by 13.4% and in Ayrshire cows by 15.0%. This is due to the fact that immunoglobulins massively migrate to secretory epithelium cells of the udder alveolar tissue. Immunoglobulin concentration in the first milking colostrum was from 57.86 g/l (Holstein cows) up to 106.78 g/l (Bestuzhev cows). In the second milking colostrum the content of immunoglobulins was decreased by 18.9% in Bestuzhev cows, by 25.1% in Black-and-White cows, by 29.3% in Holstein cows and by 21.7% in Ayrshire cows, in the forth milking colostrum the decrease was respectively by 45.4; 51.6; 52.4 and 49.2%. 6 hours after the first colostrum feeding immunoglobulin content in blood serum of calves of all breeds except for Holstein one exceeded 10 mg/ml, what was within the physiological norm. The study has shown that there were calves in every breed who did not accept immunoglobulins from the mother’s colostrum, their percentage was respectively 20; 16; 30 and 10%. The first five days of life were the most dangerous for the health of calves. During the period 6% Bestuzhev calves, 24% Black-and-White calves, 42 Holstein calves and 14% Ayrshire calves fell ill. During the fist month of life the morbidity rate of calves was respectively 16; 48; 76 and 26%.


2017 ◽  
Vol 89 (3) ◽  
pp. 1935-1942 ◽  
Author(s):  
ANA MARIA O. DIAS ◽  
LUIS F.G. DE MENEZES ◽  
WAGNER PARIS ◽  
PRISCILA V. DOS SANTOS ◽  
RONALDO R. BIESEK ◽  
...  

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