DNA degradation in post-mortem soft muscle tissues in relation to accumulated degree-days (ADD)

2011 ◽  
Vol 3 (1) ◽  
pp. e536-e537 ◽  
Author(s):  
M.S. Nazir ◽  
J.A. Smith ◽  
W. Goodwin
2021 ◽  
Vol 6 (1(Special)) ◽  
pp. 148
Author(s):  
Tania Ivorra ◽  
Anabel Martínez-Sánchez ◽  
Salvador Giner Alberola

A forensic case in which two facultative predatory species were found together on a human corpse is presented in this work. Second and third instar larvae of Synthesiomyia nudiseta (van der Wulp, 1883) (Diptera: Muscidae) and Chrysomya albiceps (Wiedemann, 1819) (Diptera: Calliphoridae) were collected from the dead body of a man on 14th November 2019, but puparia in the autopsy pictures were observed after, but not collected. The corpse was found on the terrace of the third floor of a building located in the town of Elche (SE Spain), and the pathologist confirmed the death as natural. The minimum post-mortem interval was estimated by the time to reach the length of the collected larvae or to reach the pupal developmental stage (in days and accumulated degree-days). In accordance with the influence of the predatory behaviour of each of these species on the developmental data of the other, as described in the existing literature, the effect of a hypothetical competition between both species on post-mortem interval was discussed.


2020 ◽  
Vol 315 ◽  
pp. 110419 ◽  
Author(s):  
Stephanie B. Giles ◽  
Karl Harrison ◽  
David Errickson ◽  
Nicholas Márquez-Grant

2020 ◽  
Vol 135 (1) ◽  
pp. 223-233
Author(s):  
Ann-Sofie Ceciliason ◽  
M. Gunnar Andersson ◽  
Emma Lundin ◽  
Håkan Sandler

AbstractThe objective of this study was to determine if a relationship between microbial neoformation of volatiles and the post-mortem interval (PMI) exists, and if the volatiles could be used as a tool to improve the precision of PMI estimation in decomposed human remains found in an indoor setting. Chromatograms from alcohol analysis (femoral vein blood) of 412 cases were retrospectively assessed for the presence of ethanol, N-propanol, 1-butanol, and acetaldehyde. The most common finding was acetaldehyde (83% of the cases), followed by ethanol (37%), N-propanol (21%), and 1-butanol (4%). A direct link between the volatiles and the PMI or the degree of decomposition was not observed. However, the decomposition had progressed faster in cases with microbial neoformation than in cases without signs of neoformation. Microbial neoformation may therefore act as an indicator of the decomposition rate within the early decomposition to bloating stages. This may be used in PMI estimation based on the total body score (TBS) and accumulated degree days (ADD) model, to potentially improve the model’s precision.


2018 ◽  
Vol 14 (3) ◽  
pp. 301-306 ◽  
Author(s):  
Guido Reijnen ◽  
H. Tamara Gelderman ◽  
Bernice F. L. Oude Grotebevelsborg ◽  
Udo J. L. Reijnders ◽  
Wilma L. J. M. Duijst

Insects ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 264
Author(s):  
Lorenzo Franceschetti ◽  
Jennifer Pradelli ◽  
Fabiola Tuccia ◽  
Giorgia Giordani ◽  
Cristina Cattaneo ◽  
...  

Establishing the post mortem interval (PMI) is a key component of every medicolegal death investigation. Several methods based on different approaches have been suggested to perform this estimation. Among them, two methods based their evaluation on the effect of the temperature and time on the considered parameters: total body score (TBS)/accumulated degree-days (ADDs) and insect development. In this work, the two methods were compared using the results of minPMI and PMI estimates of 30 forensic cases occurring in northern Italy. Species in the family Calliphoridae (Lucilia sericata, Calliphora vomitoria and Chrysomya albiceps) were considered in the analyses. The results highlighted the limits of the TBS/ADD method and the importance of the entomological approach, keeping in mind that the minPMI is evaluated. Due to the fact that the majority of the cases occurred in indoor conditions, further research must also be conducted on the different taxa to verify the possibility of increasing the accuracy of the minPIM estimation based on the entomological approach.


2016 ◽  
Vol 42 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Anderson Luiz Durante Danelli ◽  
Erlei Melo Reis

ABSTRACT In experiments conducted in a growth chamber, the chronological time and the accumulated degree-days were determined for the duration of incubation, latent and infectious periods of Phakopsora pachyrhizi cultivars BRSGO 7560 and BRS 246 RR. Detached soybean leaflets were placed in gerbox-type acrylic boxes and inoculated with 20 x 103 uredospores/mL. The study was conducted at 12-h photoperiod and temperatures of 10ºC, 15ºC, 22ºC, 25ºC and 30°C for 30 days. Lesions and uredia/cm2were evaluated and the number of uredia per lesion was quantified after the beginning of sporulation. The sporulation potential was also quantified for cultivars BRSGO 7560 and BRS 246 RR. The steps of the infection process can be quantified based on both the chronological time and the accumulated heat. The cultivar BRSGO 7560 produced 4,012.8 spores/cm2 and BRS 246 RR, 7,348.4 uredospores/cm2. The largest number of uredia was produced at 25ºC in both cultivars; however, BRS 246 RR presented 372.7 uredia/cm2 and BRSGO 7560, 231.6 uredia/cm2. At 10ºC and 30°C, leaf infection did not occur in both cultivars.


1994 ◽  
Vol 74 (2) ◽  
pp. 261-266 ◽  
Author(s):  
H.-H. Mündel ◽  
R. J. Morrison ◽  
R. E. Blackshaw ◽  
T. Entz ◽  
B. T. Roth ◽  
...  

Experiments were conducted for 4 yr at Lethbridge and Brooks, Alberta, and at Morden, Manitoba, to determine the effect of seeding date, cultivar (Saffire, S-208 and Cargill-3) and location on safflower (Carthamus tinctorius L.) yield, oil content, test weight and maturity; and the effect of accumulated degree–days (DD) on yield, oil content and test weight. The early-maturing cultivar, Saffire, produced the least variable yields across seeding dates. For locations and years where fall frosts did not occur before maturity, Cargill-3 produced good yields, even with late seeding; S-208 yielded less in similar situations; however, S-208 generally outyielded Saffire. Highest yields were obtained at Brooks by seeding in late April, at Lethbridge, by seeding in late April or early May. However, at both Alberta locations, Saffire could usually be seeded to mid-May without major yield reduction. At Morden, seeding during the third week of May provided optimum yields. Seeding date had little influence on oil content. Later seeding dates tended to reduce days to maturity and test weight. Safflower matured 3 wk earlier at Morden (earliest site) than at Lethbridge (latest site), with maturity at Brooks averaging 4–8 days earlier than at Lethbridge. Plants required more DD to reach maturity at Morden than at either Alberta location. DD was positively associated with yield of S-208 at both Lethbridge and Morden; for Saffire, only at Lethbridge; and for Cargill-3, only at Morden. DD generally did not significantly affect oil or test weight. Key words: Safflower, Carthamus, agronomy, seeding dates, yield, oil, test weight, maturity, degree–days


Sign in / Sign up

Export Citation Format

Share Document