Abstract Instantaneous crank power does not equal total joint power if a rider's centre of mass (CoM) gains and loses mechanical energy. Thus, estimating CoM motion and the associated energy changes can provide valuable information about cycling performance. To date, an accurate and precise method for tracking CoM motion during outdoor cycling has not been validated. Purpose: To assess the suitability of an inertial measurement unit (IMU) for tracking CoM motion during non-seated cycling by comparing vertical displacement derived from an inertial sensor mounted to the lower back of the rider to an attached marker cluster and to a kinematic estimate of vertical CoM displacement from a full-body musculoskeletal model (Model). Methods: IMU and motion capture data were collected synchronously for 10 seconds while participants (n = 7) cycled on an ergometer in a non-seated posture at three power outputs and two cadences. A limits of agreement analysis, corrected for repeated measures, was performed on the range of vertical displacement between the IMU and the two other measures. A total of 303 crank cycles were analysed. Results: The IMU measured vertical displacement of the marker cluster with high accuracy (1.6 mm) and precision (3.5 mm) but substantially overestimated the kinematic estimate of rider CoM displacement. Conclusion: We interpret these findings as evidence that a single IMU placed on the lower back is unsuitable for tracking rider CoM displacement during non-seated cycling if the linearly increasing overestimation is unaccounted for.