Lentivirus vector driven by polybiquitin C promoter without woodchuck posttranscriptional regulatory element and central polypurine tract generates low level and short-lived reporter gene expression

Ty1 activation of gene expression observed in haploid cell types of Saccharomyces cerevisiae requires the STE7 and STE12 gene products. An activator sequence within Ty1 that is responsive to these two regulators has been defined. Complex formation between a factor in whole-cell extracts and the DNA regulatory element showed the same dependence on the STE7 and STE12 gene products as did reporter gene expression. Base pair substitutions within the binding site abolished the ability to form the factor-DNA complex and to activate gene expression. The correlation between complex formation and reporter gene expression indicates that factor binding to the cis-acting element is essential for gene activation. Because the predicted protein for the STE7 gene product is homologous to protein kinases, we suggest that protein phosphorylation may directly or indirectly regulate formation of this DNA-protein complex.

Download Full-text

Lentivirus Vectors Encoding Both Central Polypurine Tract and Posttranscriptional Regulatory Element Provide Enhanced Transduction and Transgene Expression

Human Gene Therapy ◽

10.1089/104303401750214311 ◽

2001 ◽

Vol 12 (9) ◽

pp. 1103-1108 ◽

Cited By ~ 111

Author(s):

Simon C. Barry ◽

Brandon Harder ◽

Margaret Brzezinski ◽

Lisa Y. Flint ◽

Jurgen Seppen ◽

...

Keyword(s):

Transgene Expression ◽

Regulatory Element ◽

Polypurine Tract ◽

Central Polypurine Tract ◽

Lentivirus Vectors ◽

Posttranscriptional Regulatory Element

Download Full-text

Identification of a Ty1 regulatory sequence responsive to STE7 and STE12

Molecular and Cellular Biology ◽

10.1128/mcb.8.6.2545-2554.1988 ◽

1988 ◽

Vol 8 (6) ◽

pp. 2545-2554

Author(s):

M Company ◽

C Adler ◽

B Errede

Keyword(s):

Gene Expression ◽

Complex Formation ◽

Reporter Gene ◽

Regulatory Element ◽

Gene Activation ◽

Cell Types ◽

Reporter Gene Expression ◽

Regulatory Sequence ◽

Gene Products ◽

Cell Extracts

Ty1 activation of gene expression observed in haploid cell types of Saccharomyces cerevisiae requires the STE7 and STE12 gene products. An activator sequence within Ty1 that is responsive to these two regulators has been defined. Complex formation between a factor in whole-cell extracts and the DNA regulatory element showed the same dependence on the STE7 and STE12 gene products as did reporter gene expression. Base pair substitutions within the binding site abolished the ability to form the factor-DNA complex and to activate gene expression. The correlation between complex formation and reporter gene expression indicates that factor binding to the cis-acting element is essential for gene activation. Because the predicted protein for the STE7 gene product is homologous to protein kinases, we suggest that protein phosphorylation may directly or indirectly regulate formation of this DNA-protein complex.

Download Full-text