Molecular cloning and expression of Hedychium coronarium farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral and wounding/herbivory induced leaf volatile sesquiterpenoids

Gene ◽  
2013 ◽  
Vol 518 (2) ◽  
pp. 360-367 ◽  
Author(s):  
Jian-bin Lan ◽  
Rang-cai Yu ◽  
Yun-yi Yu ◽  
Yan-ping Fan
2014 ◽  
Vol 69 (5-6) ◽  
pp. 259-270 ◽  
Author(s):  
Lei Guo ◽  
Jian-Qiang Kong

Farnesyl pyrophosphate synthase (FPPS, EC 2.5.1.10) catalyzes the consecutive head-to-tail condensations of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP) to form farnesyl pyrophosphate (FPP), a key precursor of sesquiterpenoids, triterpenoids, sterols, and farnesylated proteins. Here we report the molecular cloning and functional identification of a new full-length cDNA encoding FPPS from Ornithogalum saundersiae, a potential medicinal plant that produces a promising antitumour sterol glycoside, OSW-1. An 1327 bp long unigene with an open reading frame of 1044 bp was retrieved from the transcriptome sequencing of O. saundersiae. The full-length FPPS cDNA, designated OsaFPPS, was isolated from O. saundersiae with gene-specific primers. The resultant OsaFPPS encodes a 347-amino acids protein with a calculated molecular mass of 40;085:6 Da, and a theoretical isoelectric point of 5.01. Phylogenetic tree analysis indicated that OsaFPPS belongs to the plant FPPS super-family. Expression of soluble OsaFPPS in E. coli was verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Functional analysis of the purified OsaFPPS protein was carried out using IPP and DMAPP as substrates, and the product was unambiguously determined by gas chromatography-mass spectrometry (GC-MS) analyses


PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0125415 ◽  
Author(s):  
Yu-Jun Zhao ◽  
Xin Chen ◽  
Meng Zhang ◽  
Ping Su ◽  
Yu-Jia Liu ◽  
...  

2019 ◽  
Vol 52 (3) ◽  
Author(s):  
Lin Yang ◽  
Jun Cheng Zhang ◽  
Wan Chen Li ◽  
Jing Tao Qu ◽  
Hao Qiang Yu ◽  
...  

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