Insulin-like growth factor II (IGF-II) and follicle stimulating hormone (FSH) combinations can improve the in vitro development of grown oocytes enclosed in caprine preantral follicles

2013 ◽  
Vol 23 (1-2) ◽  
pp. 37-44 ◽  
Author(s):  
Ana Beatriz Graça Duarte ◽  
Valdevane Rocha Araújo ◽  
Roberta Nogueira Chaves ◽  
Gerlane Modesto da Silva ◽  
Valesca Barreto Luz ◽  
...  
2014 ◽  
Vol 21 (2) ◽  
pp. 110-116 ◽  
Author(s):  
Sanely Lourenço da Costa ◽  
Eduardo Paulino da Costa ◽  
Emílio César Martins Pereira ◽  
Wagner Gonzaga Gonçalves ◽  
Talita Fernandes da Silva ◽  
...  

2013 ◽  
Vol 115 (1-3) ◽  
pp. 99-102 ◽  
Author(s):  
V.B. Luz ◽  
V.R. Araújo ◽  
A.B.G. Duarte ◽  
G.M. Silva ◽  
R.N. Chaves ◽  
...  

Author(s):  
Deepa Pathipati ◽  
Srinivasa Prasad Chigurupati ◽  
A.V.N. Siva Kumar ◽  
B. Punya Kumari ◽  
R.V. Suresh Kumar

Background: In in vitro culture systems IGF-I was known to promote follicular maturation, granulosa cell proliferation and increase overall cellular function. The expression patterns of developmentally important markers for IGF-I are expressed differentially during the transition of preantral follicles to the ovulatory stage. Therefore, we aimed to investigate the influence of time specific addition of Insulin like growth factor-I (IGF-I) on in vitro development of sheep preantral follicles (PFs’). Methods: Mechanically isolated sheep preantral follicles were cultured for six days either in TCM 199B or standard medium supplemented with IGF-I at different points during the culture period and were subsequently subjected to in vitro maturation (IVM) for additional 24hrs to evaluate different follicular development parameters. Conclusion: Based on the results, it is concluded that: 1) IGF-I supplementation was needed during early rather than later stages of culture period, therefore, role of IGF-I in time/stage specific manner in cultured preantral follicles is confirmed. 2) Supplementation of TCM 199B with IGF-I simultaneously for the first two days followed by TCM 199B alone without any growth factor (s) in later days (Treatment 1) supported better development of PFs’ in vitro.


2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 666-666
Author(s):  
Melba O. Gastal ◽  
Deborah M. Magalhaes-Padilha ◽  
Ana Beatriz G. Duarte ◽  
Valdevane R. Araujo ◽  
Diego D. Fernandes ◽  
...  

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