scholarly journals Association between insulin-like growth factor-I, thyroxine and follicle stimulating hormone on the survival and in vitro development of caprine preantral follicles

2014 ◽  
Vol 21 (2) ◽  
pp. 110-116 ◽  
Author(s):  
Sanely Lourenço da Costa ◽  
Eduardo Paulino da Costa ◽  
Emílio César Martins Pereira ◽  
Wagner Gonzaga Gonçalves ◽  
Talita Fernandes da Silva ◽  
...  
2013 ◽  
Vol 115 (1-3) ◽  
pp. 99-102 ◽  
Author(s):  
V.B. Luz ◽  
V.R. Araújo ◽  
A.B.G. Duarte ◽  
G.M. Silva ◽  
R.N. Chaves ◽  
...  

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 666-666
Author(s):  
Melba O. Gastal ◽  
Deborah M. Magalhaes-Padilha ◽  
Ana Beatriz G. Duarte ◽  
Valdevane R. Araujo ◽  
Diego D. Fernandes ◽  
...  

Author(s):  
Deepa Pathipati ◽  
Srinivasa Prasad Chigurupati ◽  
A.V.N. Siva Kumar ◽  
B. Punya Kumari ◽  
R.V. Suresh Kumar

Background: In in vitro culture systems IGF-I was known to promote follicular maturation, granulosa cell proliferation and increase overall cellular function. The expression patterns of developmentally important markers for IGF-I are expressed differentially during the transition of preantral follicles to the ovulatory stage. Therefore, we aimed to investigate the influence of time specific addition of Insulin like growth factor-I (IGF-I) on in vitro development of sheep preantral follicles (PFs’). Methods: Mechanically isolated sheep preantral follicles were cultured for six days either in TCM 199B or standard medium supplemented with IGF-I at different points during the culture period and were subsequently subjected to in vitro maturation (IVM) for additional 24hrs to evaluate different follicular development parameters. Conclusion: Based on the results, it is concluded that: 1) IGF-I supplementation was needed during early rather than later stages of culture period, therefore, role of IGF-I in time/stage specific manner in cultured preantral follicles is confirmed. 2) Supplementation of TCM 199B with IGF-I simultaneously for the first two days followed by TCM 199B alone without any growth factor (s) in later days (Treatment 1) supported better development of PFs’ in vitro.


2014 ◽  
Vol 34 (10) ◽  
pp. 1037-1044 ◽  
Author(s):  
Sanely L. Costa ◽  
Eduardo P. Costa ◽  
Emílio C.M. Pereira ◽  
Laércio A. Benjamin ◽  
Marcelo T. Rodrigues ◽  
...  

The aim of this study was to investigate the effects of the insulin-like growth factor -I (IGF-I) on survival, activation (transition from primordial to primary follicles) and growth of caprine preantral follicles cultured in vitro. Fragments of ovarian cortex were cultured for one and seven days in the absence or presence of IGF-I (0, 50 and 100ng/ml). The non-cultured and cultured tissues were processed and analyzed by histology and transmission electron microscopy. The culture for one day in a medium with 100ng/ml of IGF-I showed 86.7% of morphologically normal follicles. These results were similar (P>0.05) to the percentage of normal follicles found in the control (96.7%). It was also found that this medium increased the percentage of follicular activation (developing follicles) with one day of culture. The oocyte and follicular diameters remained similar to the control by culturing for one day in a medium containing 100ng/ml of IGF-I. The ultrastructural analysis did not confirm the integrity of the follicular fragments in a medium containing IGF-I (100ng/ml) after one and seven days of culture. In conclusion, this study demonstrated that the addition of 100 ng/ml of IGF-I in the culture medium enables the development of preantral follicles of goats with one day of culture. However, it is not sufficient to maintain the follicular integrity and the follicular survival rate after seven days of culture.


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