​Effects of Time Specific Supplementation of Insulin Like Growth Factor-I (IGF-I) on in vitro Development of Sheep Preantral Follicles

2021 ◽  
Author(s):  
Deepa Pathipati ◽  
Srinivasa Prasad Chigurupati ◽  
A.V.N. Siva Kumar ◽  
B. Punya Kumari ◽  
R.V. Suresh Kumar
Keyword(s):  
Growth Factor ◽  
Culture Period ◽  
Insulin Like Growth Factor ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Factor I ◽  
Igf I ◽  
Time Specific ◽  
Vitro Development

Background: In in vitro culture systems IGF-I was known to promote follicular maturation, granulosa cell proliferation and increase overall cellular function. The expression patterns of developmentally important markers for IGF-I are expressed differentially during the transition of preantral follicles to the ovulatory stage. Therefore, we aimed to investigate the influence of time specific addition of Insulin like growth factor-I (IGF-I) on in vitro development of sheep preantral follicles (PFs’). Methods: Mechanically isolated sheep preantral follicles were cultured for six days either in TCM 199B or standard medium supplemented with IGF-I at different points during the culture period and were subsequently subjected to in vitro maturation (IVM) for additional 24hrs to evaluate different follicular development parameters. Conclusion: Based on the results, it is concluded that: 1) IGF-I supplementation was needed during early rather than later stages of culture period, therefore, role of IGF-I in time/stage specific manner in cultured preantral follicles is confirmed. 2) Supplementation of TCM 199B with IGF-I simultaneously for the first two days followed by TCM 199B alone without any growth factor (s) in later days (Treatment 1) supported better development of PFs’ in vitro.

In Vitro Development of Caprine Preantral Follicles Is Improved with Insulin-Like Growth Factor-I (IGF-I).

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 666-666
Author(s):  
Melba O. Gastal ◽  
Deborah M. Magalhaes-Padilha ◽  
Ana Beatriz G. Duarte ◽  
Valdevane R. Araujo ◽  
Diego D. Fernandes ◽  
...  
Keyword(s):  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Factor I ◽  
Igf I ◽  
Growth Factor I ◽  
Vitro Development

scholarly journals Kit ligand and insulin-like growth factor I affect the in vitro development of ovine preantral follicles

2013 ◽  
Vol 115 (1-3) ◽  
pp. 99-102 ◽  
Author(s):  
V.B. Luz ◽  
V.R. Araújo ◽  
A.B.G. Duarte ◽  
G.M. Silva ◽  
R.N. Chaves ◽  
...  
Keyword(s):  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Factor I ◽  
Kit Ligand ◽  
Growth Factor I ◽  
Vitro Development

scholarly journals Influence of Insulin-like Growth Factor I (IGF-I) on the survival and the in vitro development of caprine preantral follicles

2014 ◽  
Vol 34 (10) ◽  
pp. 1037-1044 ◽  
Author(s):  
Sanely L. Costa ◽  
Eduardo P. Costa ◽  
Emílio C.M. Pereira ◽  
Laércio A. Benjamin ◽  
Marcelo T. Rodrigues ◽  
...  
Keyword(s):  
Growth Factor ◽  
Culture Medium ◽  
Insulin Like Growth Factor ◽  
Preantral Follicles ◽  
Factor I ◽  
Transmission Electron ◽  
Igf I ◽  
Growth Factor I ◽  
Vitro Development

The aim of this study was to investigate the effects of the insulin-like growth factor -I (IGF-I) on survival, activation (transition from primordial to primary follicles) and growth of caprine preantral follicles cultured in vitro. Fragments of ovarian cortex were cultured for one and seven days in the absence or presence of IGF-I (0, 50 and 100ng/ml). The non-cultured and cultured tissues were processed and analyzed by histology and transmission electron microscopy. The culture for one day in a medium with 100ng/ml of IGF-I showed 86.7% of morphologically normal follicles. These results were similar (P>0.05) to the percentage of normal follicles found in the control (96.7%). It was also found that this medium increased the percentage of follicular activation (developing follicles) with one day of culture. The oocyte and follicular diameters remained similar to the control by culturing for one day in a medium containing 100ng/ml of IGF-I. The ultrastructural analysis did not confirm the integrity of the follicular fragments in a medium containing IGF-I (100ng/ml) after one and seven days of culture. In conclusion, this study demonstrated that the addition of 100 ng/ml of IGF-I in the culture medium enables the development of preantral follicles of goats with one day of culture. However, it is not sufficient to maintain the follicular integrity and the follicular survival rate after seven days of culture.

scholarly journals Association between insulin-like growth factor-I, thyroxine and follicle stimulating hormone on the survival and in vitro development of caprine preantral follicles

2014 ◽  
Vol 21 (2) ◽  
pp. 110-116 ◽  
Author(s):  
Sanely Lourenço da Costa ◽  
Eduardo Paulino da Costa ◽  
Emílio César Martins Pereira ◽  
Wagner Gonzaga Gonçalves ◽  
Talita Fernandes da Silva ◽  
...  
Keyword(s):  
Growth Factor ◽  
Follicle Stimulating Hormone ◽  
Insulin Like Growth Factor ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Factor I ◽  
Growth Factor I ◽  
Vitro Development ◽  
Stimulating Hormone

Longitudinal bone growth in vitro: effects of insulin-like growth factor I and growth hormone

1991 ◽  
Vol 124 (5) ◽  
pp. 602-607 ◽  
Author(s):  
Ben A. A. Scheven ◽  
Nicola J. Hamilton
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Bone Growth ◽  
Long Bone ◽  
Long Bones ◽  
Insulin Like Growth Factor ◽  
Serum Free ◽  
Factor I ◽  
Igf I

Abstract. Longitudinal growth was studied using an in vitro model system of intact rat long bones. Metatarsal bones from 18- and 19-day-old rat fetuses, entirely (18 days) or mainly (19 days) composed of chondrocytes, showed a steady rate of growth and radiolabelled thymidine incorporation for at least 7 days in serum-free media. Addition of recombinant human insulin-like growth factor-I to the culture media resulted in a direct stimulation of the longitudinal growth. Recombinant human growth hormone was also able to stimulate bone growth, although this was generally accomplished after a time lag of more than 2 days. A monoclonal antibody to IGF-I abolished both the IGF-I and GH-stimulated growth. However, the antibody had no effect on the growth of the bone explants in control, serum-free medium. Unlike the fetal long bones, bones from 2-day-old neonatal rats were arrested in their growth after 1-2 days in vitro. The neonatal bones responded to IGF-I and GH in a similar fashion as the fetal bones. Thus in this study in vitro evidence of a direct effect of GH on long bone growth via stimulating local production of IGF by the growth plate chondrocytes is presented. Furthermore, endogenous growth factors, others than IGFs, appear to play a crucial role in the regulation of fetal long bone growth.

Effects of insulin-like growth factor-I on aromatase cytochrome P450 activity and oestradiol biosynthesis in preimplantation porcine conceptuses in vitro

1991 ◽  
Vol 130 (2) ◽  
pp. 245-250 ◽  
Author(s):  
A. Hofig ◽  
F. A. Simmen ◽  
F. W. Bazer ◽  
R. C. M. Simmen
Keyword(s):  
Growth Factor ◽  
Aromatase Activity ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Cytochrome P450 Activity ◽  
Igf I ◽  
Growth Factor I ◽  
Oestradiol Production ◽  
P450 Activity

ABSTRACT The effects of insulin-like growth factor-I (IGF-I) on aromatase P450 activity and steroid production in preimplantation pig conceptuses were evaluated in vitro. Conceptuses recovered from gilts on days 10 and 12 of pregnancy were incubated for 6 h in modified Eagle's Minimum Essential Medium (MEM) plus IGF-I (0·1 μg/ml) or insulin (8·5 μg/ml), and conceptuses were monitored for their ability to convert [1,2-3H]β-testosterone into oestrogens. Aromatase activity of day-10 conceptuses was low and unaffected by IGF-I or insulin. In contrast, basal aromatase activity in day-12 conceptuses was about threefold higher and was further increased by IGF-I (P < 0·02), but was unaffected by insulin. To determine whether higher aromatase P450 activity was associated with increased oestradiol production, concentrations of oestradiol were determined by radioimmunoassay in culture medium of day-11 and -12 conceptuses, after incubation in MEM alone or in the presence of dehydroepiandrosterone (DHA, 1 μg/ml) with or without IGF-I (0·1 μg/ml) or insulin (0·1 or 8·5 μg/ml) for 24 h. Conceptuses in MEM plus DHA produced more oestradiol (P < 0·01) than those in MEM alone. Addition of IGF-I or insulin did not increase the effect of DHA. Basal oestradiol production was dependent on conceptus size; however, IGF-I or insulin did not affect basal or DHA-stimulated oestradiol production regardless of conceptus size. These findings demonstrate that IGF-I can modulate aromatase activity in vitro, without affecting overall de-novo steroidogenesis. Thus, the developmental increase in conceptus oestradiol production observed during early pregnancy in the pig may reflect synergistic interactions between IGF-I and other regulatory factors present within the conceptus and/or uterine environment. Journal of Endocrinology (1991) 130, 245–250

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