Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem

2008 ◽  
Vol 31 (5) ◽  
pp. 427-433 ◽  
Author(s):  
Anna Farra ◽  
Sohidul Islam ◽  
Annelie Strålfors ◽  
Mikael Sörberg ◽  
Bengt Wretlind
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Binding Proteins ◽  
Penicillin Binding Proteins

scholarly journals Role of AmpD, OprF and penicillin-binding proteins in β-lactam resistance in clinical isolates of Pseudomonas aeruginosa

2007 ◽  
Vol 56 (6) ◽  
pp. 809-814 ◽  
Author(s):  
Simona Bratu ◽  
David Landman ◽  
Jyoti Gupta ◽  
John Quale
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Outer Membrane ◽  
Binding Proteins ◽  
Clinical Isolates ◽  
Rt Pcr ◽  
Penicillin Binding Proteins ◽  
Genomic Deletions ◽  
Cephalosporin Resistance ◽  
Outer Membrane Permeability

In this study, the mechanisms leading to increased chromosomal AmpC β-lactamase expression and the contributory roles of the outer-membrane protein OprF and penicillin-binding proteins were analysed in 33 characterized clinical isolates of Pseudomonas aeruginosa. The genes ampD and ampE were analysed by PCR and DNA sequencing. Expression of the gene oprF was assessed using real-time RT-PCR, and penicillin-binding proteins were analysed using a chemiluminescence assay. Several of the isolates with increased ampC expression had major deletions affecting ampD, although in some isolates the mechanism of increased ampC expression could not be ascertained. Occasional isolates had increased expression of both ampC and oprF but remained susceptible to cephalosporins, suggesting that increased β-lactamase activity could not offset increased outer-membrane permeability. There were no discernible changes in penicillin-binding proteins. Genomic deletions in ampD were observed in selected clinical isolates of P. aeruginosa with increased expression of the AmpC β-lactamase. For some isolates, cephalosporin resistance was dependent upon the interplay of ampC and oprF expression.

Proteomic comparison of outer membrane protein patterns of sessile and planktonic Pseudomonas aeruginosa cells

Biofilms ◽  
2005 ◽  
Vol 2 (1) ◽  
pp. 27-36 ◽  
Author(s):  
D. Seyer ◽  
P. Cosette ◽  
A. Siroy ◽  
E. Dé ◽  
C. Lenz ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pseudomonas Aeruginosa ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Two Dimensional ◽  
Protein Patterns ◽  
Statistical Selection ◽  
Selection Threshold

We have compared the outer membrane protein (OMP) pattern of sessile Pseudomonas aeruginosa cells with that of its stationary-phase free-floating counterparts. Two-dimensional electrophoretic gels were compared using computing scanning densitometry. The level of 103 OMPs (i.e. about 50% of the spots that fulfilled our statistical selection threshold) was significantly and reproducibly different in biofilm organisms as compared with their planktonic counterparts. Some OMPs that specifically either accumulated or decreased were identified by mass spectrometry and the role of these proteins in the biofilm phenotype is discussed.

scholarly journals Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e46857 ◽  
Author(s):  
Horst Funken ◽  
Kai-Malte Bartels ◽  
Susanne Wilhelm ◽  
Melanie Brocker ◽  
Michael Bott ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Specific Association
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