Comparison of Disk Diffusion and Broth Microdilution Methods for in Vitro Antifungal Susceptibility Testing of Dermatophytes

Female Genital Tract ◽

In Vitro Antifungal Susceptibility

Vein Thrombosis ◽

Microbial Infections ◽

Life Threatening ◽

Introduction: Endometrial cancer is one of the most common malignancies of the female genital tract, which can be serious or life-threatening. Microbial infections can be one of the underlying causes of this type of cancer. Case Presentation: The present study describes the isolation of Pichia fermentans (Candida firmentaria var. firmentaria) from the vaginal secretions of a 61-year-old woman affected by endometrial cancer. She reported abdominal pain and vaginal discharge for 3 months, and had a history of diabetes, hypertension, Deep Vein Thrombosis (DVT), and Acute Myeloid Leukemia (AML). The isolated yeast was identified based on nuclear ribosomal internal transcribed spacer (ITS1-ITS2 rDNA) sequence analysis. The in vitro antifungal susceptibility testing showed a higher effect for ketoconazole against P. fermentans than fluconazole, itraconazole and voriconazole. Conclusion: Correct differentiation between P. fermentans and other yeast should be considered. The in vitro antifungal susceptibility testing is recommended for rare yeast, and will help the physicians in providing the best treatment.

Isolation, Identification, and In Vitro Antifungal Susceptibility Testing of Dermatophytes from Clinical Samples at Sohag University Hospital in Egypt

Electronic physician ◽

10.19082/2557 ◽

2016 ◽

Vol 8 (6) ◽

pp. 2557-2567 ◽

Cited By ~ 8

Author(s):

Mona Fattouh Mohamed Shalaby ◽

Asmaa Nasr El-din ◽

Mohammed Abu El-Hamd

Keyword(s):

Susceptibility Testing ◽

In Vitro Antifungal Susceptibility

University Hospital ◽

Clinical Samples ◽

Standardized Method for In Vitro Antifungal Susceptibility Testing of Candida albicansBiofilms

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.45.9.2475-2479.2001 ◽

2001 ◽

Vol 45 (9) ◽

pp. 2475-2479 ◽

Cited By ~ 464

Author(s):

Gordon Ramage ◽

Kacy Vande Walle ◽

Brian L. Wickes ◽

José L. López-Ribot

Keyword(s):

Antimicrobial Agents ◽

Susceptibility Testing ◽

Colorimetric Assay ◽

In Vitro Antifungal Susceptibility

Phenotypic Traits ◽

Salt Reduction ◽

Treatment Regimens ◽

ABSTRACT Candida albicans is implicated in many biomaterial-related infections. Typically, these infections are associated with biofilm formation. Cells in biofilms display phenotypic traits that are dramatically different from those of their free-floating planktonic counterparts and are notoriously resistant to antimicrobial agents. Consequently, biofilm-related infections are inherently difficult to treat and to fully eradicate with normal treatment regimens. Here, we report a rapid and highly reproducible microtiter-based colorimetric assay for the susceptibility testing of fungal biofilms, based on the measurement of metabolic activities of the sessile cells by using a formazan salt reduction assay. The assay was used for in vitro antifungal susceptibility testing of severalC. albicans strains grown as biofilms against amphotericin B and fluconazole and the increased resistance of C. albicans biofilms against these antifungal agents was demonstrated. Because of its simplicity, compatibility with a widely available 96-well microplate platform, high throughput, and automation potential, we believe this assay represents a promising tool for the standardization of in vitro antifungal susceptibility testing of fungal biofilms.

Etest Cannot Be Recommended forIn VitroSusceptibility Testing of Mucorales

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00004-15 ◽

2015 ◽

Vol 59 (6) ◽

pp. 3663-3665 ◽

Cited By ~ 20

Author(s):

Rita Caramalho ◽

Elisabeth Maurer ◽

Ulrike Binder ◽

Ricardo Araújo ◽

Somayeh Dolatabadi ◽

...

Keyword(s):

Amphotericin B ◽

Antimicrobial Susceptibility ◽

Active Drug ◽

Susceptibility Testing ◽

Broth Microdilution ◽

European Committee ◽

Susceptibility Patterns

ABSTRACTAmphotericin B and posaconazole susceptibility patterns were determined for the most prevalent Mucorales, following EUCAST (European Committee on Antimicrobial Susceptibility Testing) broth microdilution guidelines. In parallel, Etest was performed and evaluated against EUCAST. The overall agreement of MICs gained with Etest and EUCAST was 75.1%; therefore, Etest cannot be recommended for antifungal susceptibility testing of Mucorales. Amphotericin B was the most active drug against Mucorales speciesin vitro, while the activities of posaconazole were more restricted.